LOCUS       XM_070514256            9369 bp    mRNA    linear   MAM 18-DEC-2024
DEFINITION  PREDICTED: Equus asinus dicer 1, ribonuclease III (DICER1),
            transcript variant X10, mRNA.
ACCESSION   XM_070514256
VERSION     XM_070514256.1
DBLINK      BioProject: PRJNA1198117
KEYWORDS    RefSeq.
SOURCE      Equus asinus (donkey)
  ORGANISM  Equus asinus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_091796) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_041296235.1-RS_2024_12
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 12/16/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Equus asinus"
                     /mol_type="mRNA"
                     /isolate="D_3611"
                     /db_xref="taxon:9793"
                     /chromosome="7"
                     /sex="male"
                     /tissue_type="Whole blood in anticoagulant"
                     /geo_loc_name="USA: Ithaca, New York"
                     /collection_date="2018-03-01"
                     /breed="Donkey"
     gene            1..9369
                     /gene="DICER1"
                     /note="dicer 1, ribonuclease III; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 148
                     long SRA reads, 1 Protein"
                     /db_xref="GeneID:106832636"
     CDS             90..5042
                     /gene="DICER1"
                     /codon_start=1
                     /product="endoribonuclease Dicer isoform X4"
                     /protein_id="XP_070370357.1"
                     /db_xref="GeneID:106832636"
                     /translation="

MELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIEPVVDDDDVFPPYVLRPEDGGPRVTVNTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISTANSSSAENDSYCKHSTIVVPEHAAHPGANRAPSPEHRDQMSVSCRTLLSRPPGELPTEVSADLAAVNGLSYNGNLANGSYDLANRDFCQGNQLNDYKQEIPVQPTTSSPIQSLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPLMAATPGTAEAVKALKARADSEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDETTKDCMLANGRLDRGLEEEEEEDLLWRAPREDADDDDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKGLCPPRENFSSQQKNLSGGWAAASAASSRSSVCKDLEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEMVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS"

     misc_feature    90..371
                     /gene="DICER1"
                     /note="Partner-binding domain of the endoribonuclease
                     Dicer; Region: Dicer_PBD; cd15903"
                     /db_xref="CDD:277191"
     misc_feature    order(102..107,114..116,120..125,300..305,312..317,
                     324..326,333..338,345..347)
                     /gene="DICER1"
                     /note="Trbp binding interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:277191"
     misc_feature    390..968
                     /gene="DICER1"
                     /note="C-terminal helicase domain of the endoribonuclease
                     Dicer; Region: SF2_C_dicer; cd18802"
                     /db_xref="CDD:350189"
     misc_feature    order(621..629,837..839,894..896,900..902)
                     /gene="DICER1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350189"
     misc_feature    order(849..851,855..857,930..932,936..938)
                     /gene="DICER1"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:350189"
     misc_feature    1164..1430
                     /gene="DICER1"
                     /note="Dicer dimerization domain; Region: Dicer_dimer;
                     pfam03368"
                     /db_xref="CDD:460900"
     misc_feature    1932..2300
                     /gene="DICER1"
                     /note="PAZ domain, dicer_like subfamily. Dicer is an RNAse
                     involved in cleaving dsRNA in the RNA interference
                     pathway. It generates dsRNAs which are approximately 20 bp
                     long (siRNAs), which in turn target hydrolysis of
                     homologous RNAs. PAZ domains are named...; Region:
                     PAZ_dicer_like; cd02843"
                     /db_xref="CDD:239209"
     misc_feature    order(2121..2123,2160..2162,2178..2180,2190..2192,
                     2244..2246,2268..2270,2274..2276)
                     /gene="DICER1"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239209"
     misc_feature    3165..>3440
                     /gene="DICER1"
                     /note="Ribonuclease III family; Region: RIBOc; smart00535"
                     /db_xref="CDD:197778"
     misc_feature    order(3216..3221,3228..3233,3240..3242,3249..3254,
                     3261..3266,3270..3278,3306..3308)
                     /gene="DICER1"
                     /note="dimerization interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:238333"
     misc_feature    4317..4811
                     /gene="DICER1"
                     /note="Ribonuclease III C terminal domain. This group
                     consists of eukaryotic, bacterial and archeal ribonuclease
                     III (RNAse III) proteins. RNAse III is a double stranded
                     RNA-specific endonuclease. Prokaryotic RNAse III is
                     important in post-transcriptional...; Region: RIBOc;
                     cd00593"
                     /db_xref="CDD:238333"
     misc_feature    order(4377..4382,4389..4394,4401..4403,4410..4415,
                     4422..4427,4431..4439,4467..4469,4731..4733,4764..4766)
                     /gene="DICER1"
                     /note="dimerization interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:238333"
     misc_feature    order(4377..4379,4386..4388,4398..4400,4701..4703,
                     4710..4712)
                     /gene="DICER1"
                     /note="active site"
                     /db_xref="CDD:238333"
     misc_feature    order(4386..4388,4701..4703,4710..4712)
                     /gene="DICER1"
                     /note="metal binding site [ion binding]; metal-binding
                     site"
                     /db_xref="CDD:238333"
     misc_feature    4824..5012
                     /gene="DICER1"
                     /note="double-stranded RNA binding motif of
                     endoribonuclease Dicer and similar proteins; Region:
                     DSRM_DICER; cd10843"
                     /db_xref="CDD:380680"
     misc_feature    order(4824..4829,4833..4838,4845..4850,4857..4862,
                     4872..4874,4887..4895,4899..4901,4905..4907,4956..4967,
                     4974..4976)
                     /gene="DICER1"
                     /note="putative RNA binding site [nucleotide binding];
                     other site"
                     /db_xref="CDD:380680"
     polyA_site      9369
                     /gene="DICER1"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

tcttggacagatacacttctcagccatgtgagatcgtggtagactgtggaccatttactgacagaagcgggctttatgaaagactgctgatggagttagaagaagcacttaatttcatcaatgactgtaacatatctgtacattcaaaagaaagagattctactttaatttctaaacagatcctgtcagactgtcgtgcggtattggtggttctgggaccctggtgtgctgataaagtggctggaatgatggtaagagagctacagaagtacatcaaacatgaacaggaggagctgcacaggaaattcctattgtttacagatactttcctgaggaagatccacgcactctgtgaagagcacttctcacctgcctcacttgacctgaaatttgtaactccgaaagtcataaaactgcttgaaatcttacgcaaatataaaccatatgagcgacagcagtttgagagtgttgagtggtataataataggaatcaggataattatgtgtcttggagtgattctgaggatgatgatgaggatgaagaaattgaagaaaaggagaagccagagacaaattttccttctccatttaccaatattttatgcggaattatttttgttgaaagaagatatacagcagttgtcttaaacagattgataaaggaagctggcaaacaagatccagagctggcttacatcagcagcaattttataactggacatggcatcggaaagaatcagcctcggaacaaacagatggaagcagaattcagaaaacaggaagaggtacttaggaaatttcgagcacatgagaccaacctgcttattgcaacaagtatcgtggaagagggtgttgatatcccaaagtgcaacttggtggttcgttttgatttgcccacagagtatcgatcctacgttcaatctaagggaagggcaagggcaccaatctctaattatataatgttagcagatacggacaaaataaagagttttgaagaagaccttaaaacgtacaaagctattgaaaagatcttgagaaacaaatgttccaagtcagttgatactggtgagactgacattgagcctgttgtagatgatgatgacgttttcccaccgtacgtgttgaggcctgaggatggtggcccgcgagtcacagtcaacacggccattggacacatcaatagatactgtgctagattaccaagtgatccatttactcatctggctcctaaatgtagaacccgagagttgcctgatggtacattttattcaactctttatctgccaattaactcgcctcttcgagcctccattgttggtccacccatgagctgtatacgactggctgaaagagttgtagctctcatttgctgtgaaaaactgcacaaaattggtgaactggacgaccatttgatgccagttgggaaagagactgttaaatatgaagaagagcttgatttgcatgatgaagaagagaccagtgttccaggaagaccaggttcaacaaaacgaagacagtgctacccaaaagcgattccagaatgtctgcgggatagctatcccaaaccggaccagccctgttacctgtacgtgataggaatggttctgaccacacctctacctgatgaactcaacttcagaaggcggaagctctatccccctgaggacaccacaagatgctttggaatactgacagccaaacccatacctcagattccgcactttcctgtgtacacccgctctggagaggttaccatatccattgagttgaagaagtctggtttcacgttgtctctgcaaatgcttgagttaattacaagacttcaccagtatatattctcgcatattcttcggcttgaaaaacctgcactagagtttaaacccgcagacgctgactcagcatactgcgttctacctctcaatgttgttaatgactccagcactttggacattgactttaaattcatggaagatattgagaaatctgaagctcgcataggcattcccagtacaaagtattcaaaggaaacaccctttgtttttaaattagaagattaccaggacgcagttatcattccaagatatcgcaattttgatcagcctcaccgattttatgtagctgatgtgtacactgatcttaccccactgagtaaatttccttcccctgagtatgaaacttttgcagagtattataaaacaaagtataaccttgacctgaccaacctcaaccagccgctgctggatgtggaccacacatcttcaagacttaatcttctgacacctcgccatctgaatcagaaagggaaagctcttcctttaagcagtgctgagaagaggaaagccaaatgggaaagtctgcagaataaacagatcctggttccagaactctgtgctatccatccaattccagcgtcactgtggagaaaagcggtttgtctccccagcattctctatcgccttcactgccttctgaccgcagaggagctccgagcccagacggccagcgatgctggcgtgggagtccgatcgctccccgtggattttagataccctaacttagactttgggtggaaaaaatctatcgacagcaaatctttcatctcaactgctaactcctcttcagctgaaaacgatagttactgtaagcacagcacaattgtagtccctgaacatgctgcacatccaggtgctaatagagccccctctccagaacaccgtgaccaaatgtctgtgagctgcaggacgctgctcagccggccgcccggtgagctcccaactgaagtttcagcagatctggcggcagttaatggtctttcttacaatggaaatcttgccaacggcagttacgatttagctaacagagacttttgccaaggaaatcagctgaatgactacaagcaggaaatacctgtacaaccaactacctcatctcccattcagagtttatacaattacgagaaccagcccaagcccagcgatgaatgtactctactgagtaataaataccttgatggaaatgctaacacatctacctcagatggaagtcccctgatggccgcgacgcctggtactgcagaagctgtgaaagcgctcaaggccagggcggattctgagcagagtccttctcctgggtactcctcgaggactctgggcccaaatccaggacttattcttcaggctctgaccctttcaaatgctagcgatgggtttaacctggagcggctggaaatgcttggagactcctttttgaagcatgccatcaccacctatctattttgcacttatcctgacgctcacgaaggccgcctttcatacatgagaagcaaaaaggtcagcaactgtaatctctatcggcttggaaaaaagaagggactgcccagccgcatggtggtgtcgatatttgatccccctgtgaattggcttcctcctggttatgtagtaaatcaagacaaaagtaacacagataaatgggaaaaagatgaaacgacaaaagactgcatgttggctaatggcagactggaccggggcttggaggaggaggaggaggaggacctgctctggagggctccgagggaggacgccgacgacgacgacgatttcctggagtacgatcaggagcatatcaagttcatagacaacatgttaatgggatctggagcttttgtcaagaaaatttctctttctcctttttcaaccactgattctgcatatgaatggaaaatgcccaaaaaatcctccttaggtagtatgccattttcatccgattttgaggattttgactatagctcttgggatgcaatgtgctatctggatcctagcaaagctgttgaagaggatgactttgtggtggggttctggaatccgtcagaagaaaactgtggtgttgacacagggaagcagtccatttcttacgacttgcacactgagcagtgcattgctgacaaaagcatagccgactgtgtggaagcgctgctgggctgctatttaaccagctgtggcgagagggccgctcagcttttcctctgctccttggggctgaaggtgctcccggtaattaaacggactgaccgggaaaagggcctgtgcccgcctagggagaatttcagcagccaacaaaagaacctttcagggggctgggctgcggcctctgcagccagctctcgctcttctgtctgtaaagacttggagtacggttgtctgaagatcccaccgaggtgtatgtttgaccatccagacgcagataaaaccctgaatcaccttatatcgggttttgaaaattttgaaaagaaaatcaactacagattcaagaataaggcttaccttcttcaggctttcacacacgcctcctaccactacaataccattactgattgttaccagcgcttagagttcctgggagatgcgattttggactacctcatcaccaagcacctttacgaagacccccggcagcactccccaggggtgctgaccgacctgcggtctgctctggtcaacaacaccatctttgcgtccttggctgtcaagtacgactaccacaagtacttcaaggcagtgtctcctgagctcttccacgtcatcgacgacttcgtgcagttccagcttgagaagaacgagatgcagggaatggattctgagcttaggagatctgaggaggatgaagagaaggaagaggacattgaagttccaaaggccatgggggatatttttgagtcgcttgctggtgccatttacatggatagcgggatgtccctggagatggtttggcaggtgtactaccctatgatgcggccactcatagaaaaattttctgcaaatgtgccccgttcccctgtgcgagaattgcttgaaatggaaccagaaactgccaaatttagcccggctgagagaacttacgatggcaaggtcagagtcaccgtggaagtagtgggaaagggaaagtttaaaggtgttggccgaagttacaggattgccaaatctgcagccgcaagaagagccctacgaagcctcaaagctaatcaacctcaggttcccaacagctgaaacccctttttaaaattaaaaaaaaaaagcagaattaaggtggaaaatatttaaattgaaagggatttaaagttgatgctgagtggaataaattgaaggcagaatttaaagtttgataacaagctaggttacagaataaaacatttaacatgtgtataaaaattttggaaactaattgtagttttagttttttgcgcaaacacaatcttgtcttctttccttctgctccgtttaaatcacaagagtgctttaatgatgacatttagcgagtgggcagaataattaccgacagttttgttctgtgttttttgttttttagttgggtttccgtcagcttagctcttagtgttctgttaggaggccaaggagcttaccctcccgcagtcccggaactgccgccgtgctgacgtgcctccatctgtgcggtcgccgtcccacatggctggcaagcccagtagagtgacgcggttctcgagaggagaagtgtgcccgtgttgtcttttctttccatgttatatcgtgtaagatgacgtctcctggtcacgattgcacttaatagaatggacagattgtaattaacgttggctggcacgttggtgaatcaaattttaattttctcatgccacgtagtcttgcataaagaaggttcttgccttaaaattaaacccctcatggctattctttagtttctgatcttttcggaacaaactattttacattcccttcatgttattatgcatcagatgttgagacagcgtaatatttacaactctctagtctagctgtaacttaacttattacaggattatattaggatttctgttatatgtgtactgaagacattttaaaaaccagagtatgtagtctatggatttttttaaacactgtaataaaaatggtgtttggctacatacccaattttactaaaaacctcctgctaggtagttccacggatggaaattgtttgtggcaaataattttgccctgtaggctgttgctctaacaaaataaactttagacatatcacacctaaaatatgctgcagattttataattgattggttacttatttaaagaagcgaaacagagcaccttgacccttagtcttcttgcatacatctcttactgtacttttcacgatgttgcatgcatatttcacctaccaaagctgtgctgttaatgccatggaagtttaatgtctgcaacaaactgccgtaattttgatacatctgtgatttaggtcattaatttagataaactagctcattatttccatctttggaaaagagaaaaaaagacatttttaggcatttgcctaagtttctttaattagacttgtagggcactcttcacttaaatacctcagttcttcttttcttttgcatgcatgtttttcccctgtttggtgctatgtttatgtatatgcttgaaattttaatttttttttttttgcactgtaactataatacctcttaatttacgtttttaaaagctgtgggtcagtcttgcactcccaccaacataccagtagaggtttgctgcaatttgccccgttaattatgcttgaagtttaagaaagctgagcagaggtgtctaatactttccagcacatcattctgaacccgatgcttcatgtaatgctgcatttatgtttcgaatttcagtcgaatactgtacttcttgctctgtctgcaccccccaccccagggaaacacctctgcacgtgtgcacacagaaaacggtcagctccgcctcctcaggaaccctgagtgaggggatgcgcagcctctccaacatggtctgctgtctccaatcttaaactgaagtcaagatctaaattattaaatgggtttctgagcaaattaattcaggtggactcatttttaaaaatattcaattctgatttggaaccttagatttctatttgttttttttttcaaaaaacctcaatgtaaggtctcctggcaagttaaaaccaagcaattcttgaattacagaactgtaaacgtgtacaattagcaagtaaggctggatgtgaattttactcgtgagggtgatttgtgatgaagttatgtcacaaatctcgtgatgatttataaactacctgatgccaggagctgagagctttgcattgcgtctaatgcactcatcccagtgttaagggactcgctcgcctcctggcaccaaaatcagattgtttcacagttacaattcccgttgggagaaaaatgcctcaatatattttgtaaccttaaggagagtattttttgttaatactaaaaagttcagacttgggtatgattaggttataacattctcaggggttcaaatttcctgctaccattcaaaatgtttatcaacagcaaactttagctatttctttttttttttttttaggttggagaaaaatagctgatcttaatttgtgactcatgctttgaagcattctctgatcccctggttactagttaaaaaataaaaactatgagttagacatacgaaatggttaggaacgctttcgtgctgctgatttttaatgctgtaaagtttcccccagtttagcttgttgaaatgttttgcatccattaattaaggaaaaactcagtctgtgttgccccactttccaactgtgtgtgtcacccttttgttcctgtgattgcaatgtgcgaccgaatgtaatatggaaacctgtactagtgggaggtgtgattgtgcccaagcacacgtggaccggactggagaagcgtgtcttgaaaaagcaactgcagaaattccttgcgatgattgtgtggaggttattggcacgagccttcattgtaaatgtttttaatttctttataatatactttcagcagtcctaactatgctgcgttttgtaatagctttcttcccccgttctgtccgtgtagcacagataaacatggcccttggtaccgcgctttacctcatttcaaggaaaatatgcttaactgagaggaaaaatgtggtttggcctcgctgctgttttgatttaccgaatttgaaaaagataattataatgcctgcaatgtgtcatatacttgcacaacttaaataggtcatttttgtctgtggcatttttactgtttgtgaaagtatgacaaagatttgttaactgaactcttaattatgttggaaaaatgtttgttacagttttttcttttttcttttttttttttttacattatgtgaagtgattaaatttagaatgacctctaacactcctgtaattatattttaagatactaatgtttttactttcttaatgatagcctgccctcggaaagattcagatgagcctgcctagatagcaaggacttgcggctgccttggttcagctggttcagttcataaatgcagttactcacgcagcagccagctcttccagtcgttacctggaatcagacggtgcctgcagagagcagagaccatcgaggtgcagccccgccacaggccagtctgaaggcctggttgatttaaaacttgatcccctgctctgttgggctatctaatcctttcacacaaaatttcagccaccgaactgagaccttgatgtctttatttccactgcatccgaaatcgtgtgagagtccttcctagttagtgtcacctgcagaagagaaactcctgtcacgtttgttaccatgcggttattttaattacatttaaataggtatattttttcaaccactgattactgtttaggaatttaattatttccaaataaatttctttattttatattgtacatgaaaagtttttaagatatgtttaagaccaagaatattaaaatgattttaaaagttgttgggagacgccagtagcaatacctagggaatttgcatcgagactgttgtgttttccgctagcagtgaaaatgatcttttggaactaacttgtaaatatattttaatcgttaattgtttttttcccttgtccgtttttatttgggcatccaccacagacaatttaaatttcgttttaataaagatgcgctcagaacgcagcagcagggtacgtagcagaaatgcaaaggtggacaggtagtacaattgctggcttttctgctgtaaatagaatgaaggaaaatgactcaaatcaagtaaaactaacacacatgatttgattgacaataaagtatttgccatcacatgctgcagctgtttgtcaggaatatggtgtcattcattcattcagtaatcacgctgcagaaattggcagcctgcaccttatgagtcacattacctttagtcgtttttttgtattaattgtagccaagtaaatcttcaataaagttatggtctgttca

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