LOCUS       XM_067361582            3210 bp    mRNA    linear   VRT 19-AUG-2024
DEFINITION  PREDICTED: Chanodichthys erythropterus argonaute RISC component 4
            (ago4), transcript variant X9, mRNA.
ACCESSION   XM_067361582
VERSION     XM_067361582.1
DBLINK      BioProject: PRJNA1148501
KEYWORDS    RefSeq.
SOURCE      Chanodichthys erythropterus (predatory carp)
  ORGANISM  Chanodichthys erythropterus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Xenocyprididae; Xenocypridinae; Chanodichthys.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_090235) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_024489055.1-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/16/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Chanodichthys erythropterus"
                     /mol_type="mRNA"
                     /isolate="Z2021"
                     /isolation_source="lake"
                     /db_xref="taxon:933992"
                     /chromosome="15"
                     /tissue_type="muscle"
                     /geo_loc_name="China: Hulun lake"
     gene            1..3210
                     /gene="ago4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 13
                     Proteins"
                     /db_xref="GeneID:137002086"
     CDS             145..2784
                     /gene="ago4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X9"
                     /protein_id="XP_067217683.1"
                     /db_xref="GeneID:137002086"
                     /translation="

MEALGPGPPAPPSLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVSVVSLQMLLEALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRVSTDTGRDCSRGLSPQNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDLSQEQLFSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA"

     misc_feature    226..615
                     /gene="ago4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    646..798
                     /gene="ago4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    799..1161
                     /gene="ago4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(955..957,1000..1002,1042..1044,1054..1056,
                     1108..1110,1129..1131,1135..1137)
                     /gene="ago4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1300..2655
                     /gene="ago4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1759..1761,1771..1773,1807..1818,1825..1827,
                     1849..1851,1858..1860,1870..1872,1882..1884)
                     /gene="ago4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1963..1965,1969..1971,2209..2211,2623..2625)
                     /gene="ago4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

agacaacgcaagcacagcgctacacatctacgcatccttccatttcttaaattagccaccttaagcttcgagataccgcccccaatttcagtgtcggtacggagccgccaggccgggacagagacggagagagatccctcggccatggaagcgctcggacccggcccgcctgcccctccctccctcttccagccaccgcgccggcccggattgggtacggtggggaagccgattcgcctgctggccaatcacttccaggtgcagatccccaagatcgacgtctaccattacgacatcgacatcaagcctgaaaagcgaccccgcagggtcaacagagaggtggtggacacgatggtgagacactttaagatgcagatcttcggggacagacagcctgggtatgatggcaagaggaacatgtatacggcgcatccgctacccatcggccgggaccgggtggatctggaggtgacgttgccaggcgagggcaaggatcagaccttcaaggtgtctctgcagtgggtgtcggtggtgagtctgcagatgctcctggaagctctgtccggtcacctgaacgaggtcccggaggactctgttcaggctctggatgtcatcactcgtcacctgccctccatgcggtacactccagtcggtcgttctttcttctcaccaccagagggatactatcacccgctgggaggagggagggaggtttggttcggtttccatcagtcggtccgtccagcgatgtggaacatgatgctcaacatagatgtgtcagccacagccttctaccgagcccagcctgtcatcgagttcatgtgtgaggttctggacatccagaacatcaatgagcagaccaaacccctcacagattcacaacgtgtcaagttcaccaaggagatccgaggactgaaagtggaggtcacacactgcggccagatgaagaggaagtaccgtgtgtgtaacgtcacgcgccgccctgccagccatcagacatttcctttgcagcttgagaacggacaagctatggagtgcaccgtcgcccagtatttcaaacaaaagtacagcctgcagctcaaatacccccatctgccctgcctccaggtggggcaggaacaaaagcacacctacctgccccttgaggtttgtaacatagtggcgggacagcgctgtataaagaagctaaccgataaccagacctcgaccatgatcaaagctacggcccgctccgccccagacagacaggaagagatcagcagactggtcaaaagcaacagcatggtgggcgggcccgacccgtacctgaaagagttcggtatcgtggtgcacaatgacatgaccgaggtgactgggcgggtcctcccagcgcccatgcttcagtatgggggccgggtgagtacagacactgggagggactgtagcaggggactctctcctcagaacaagacggtggccacacccaaccagggtgtctgggacatgagaggaaagcagttttatgcgggtattgagatcaaggtgtgggccgtcgcctgcttcgccccgcagaaacagtgcagagaggatctgctcaagagcttcaccgaccagctgcgcaagatctccaaagatgccgggatgccgattcaaggccagccctgcttctgcaagtacgcccagggcgcagacagcgtggagcccatgttcaagcatctcaagatgtcttacgtgggactacagctcattgtggtcattctgccggggaaaacgcccgtctatgcggaggtgaagcgtgtgggagacactctgctgggaatggccactcagtgcgtgcaggtgaaaaacgtggtgaagacgtcccctcagacgctgtccaacctctgtctgaagatcaacgccaaactagggggcatcaacaatgtgctggtgccacatcagaggccctctgtgttccagcagccggtcatcttcttgggcgcagatgtcactcacccccccgcgggcgacgggaagaagccgtccattgcggcggtggtggggagcatggacggacaccccagccgctactgtgccaccgtgcgggtgcagacttcacgccaggatctgtcccaggagcagctcttcagtcaggaggtcatccaggatctcaccaacatggtgcgagagctcctcattcagttctacaagtcgacccggtttaagcccacgcgcatcatctactaccgcggaggagtttctgagggccagatgaagcaggtcgcctggccggagctgatcgccatcaggaaagcttgcatcagtctcgaagaggattacagaccgggaatcacctacatcgtagtgcagaaacgtcaccacactcgcctcttttgctctgataaagccgagagggtcgggaagagtggcaatgtcccagcaggcactacagtggacagcaccatcacacacccctcagagtttgacttctacctgtgcagccatgctggaatccagggcactagccgtccctctcactatcacgtcttgtgggatgacaactgtttcaccgccgacgaactgcagctcttgacttaccagctctgccacacctacgtgcgctgcacccgctccgtctccatcccagcgccagcctactacgcccggctcgtggcgttccgtgcccgttaccacttggtggacaaagaccatgacagcgctgaaggaagtcacgtatcaggacagagtaacggccgagaccctcaggccctggcgaaggcggtccagattcactatgatacccagcacactatgtacttcgcctgacgtcagccagcaggagacgcacttctacactccctcttcgatctgtctctctctctcttcctcttcctgtttctctatcgcgtcgtcgtctgcagccaagcagttctgaacccagggggccatctgtcaatccagtcgaaatcggtcggcctcggaagaaccaacctctaccagcagtcctgcactgagaggcttcctgcctccaatgcacagagaaaaaaatcaatttgagccatttttaacactttcttttcttgttttgattttgaatgtttttaatttgtaatatacactgagtgtgagcaaacctgcgccattggtcagggcagctctggctctcttagctaagctggactctgtctacttttacctcaagccgcaattggctgaatcttgtcacatgtcttttggggtggggtggggcgg

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