LOCUS       XM_066706393            6476 bp    mRNA    linear   VRT 30-SEP-2025
DEFINITION  PREDICTED: Amia ocellicauda ATPase copper transporting beta
            (atp7b), transcript variant X2, mRNA.
ACCESSION   XM_066706393
VERSION     XM_066706393.1
DBLINK      BioProject: PRJNA1136883
KEYWORDS    RefSeq.
SOURCE      Amia ocellicauda
  ORGANISM  Amia ocellicauda
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Holostei; Amiiformes; Amiidae; Amia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_089855) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_036373705.1-RS_2025_09
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 09/26/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Amia ocellicauda"
                     /mol_type="mRNA"
                     /isolate="fAmiCal2"
                     /db_xref="taxon:2972642"
                     /chromosome="6"
                     /sex="female"
                     /tissue_type="blood, heart, muscle, misc"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Vacherie, Louisiana"
                     /lat_lon="29.929889 N 90.747444 W"
                     /collection_date="2023-03-15"
                     /collected_by="Ingo Braasch, Allyse Ferrara"
     gene            1..6476
                     /gene="atp7b"
                     /note="ATPase copper transporting beta; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 25 Proteins"
                     /db_xref="GeneID:136751095"
     CDS             92..4435
                     /gene="atp7b"
                     /codon_start=1
                     /product="copper-transporting ATPase 2 isoform X2"
                     /protein_id="XP_066562490.1"
                     /db_xref="GeneID:136751095"
                     /translation="

MYGAVGTTSRVYQEGLENGSAVGPLQSHAFQNLGYEPGSQSQIYPPPSDLSQATIDITGMTCQSCVQSIEGTVSKVKGVVAIKVSLANSNAQLQYNTSTITAQDVCQQIGNMGFDARVVSSMAASGSISSLMESVVKIQVEGMTCQSCVNSIEGQVGKLQGVLRIRVSLGRQEAVITYQPCFIQPENLRSHIEEMGFEATIKTKPQPLRLAPSEVERLQQDNAQNARRVDRSGTPGTAADSHRATLVVEGMHCSSCVHNIRGHLSEITGVQSITVSLEERSIDLQYNPSVVTLESVKRVIEGLPPGNFRVSLPCGMSDLSAAAPLSVTDPGFNDGGGLVRRTLINIRGMTCNSCVQSIEGMITQRTGVRSIQVSLAEEKGTILYEHTLTSPDELRDTIEDMGFEASIAVTPEAPATKQRSTQNQSFVLKQDSPCHTLPGTVSQSLPPPQVQPQTPERESQRSEEGRTQKCFIHVTGMTCASCVANIERKLQRHRGVVSVLVALMAGKAEVKYDPQVLNAAQITQLIISLGFGAKLIEDHAIMDGKLDLVVTGMTCASCVHNIETRLMSTKGILEASVALATSKAHIKFDPDVLGARNIIRIIEGLGFGAALMKNEGVGNNMDHQKEIQQWKNSFLFSLVFGIPVMGLMIYMIVMDSKHSGSMPKEQNIVPGLSILNLAFFVLCTPVQFLGGWYFYVQAYKSLKHKTANMDVLIVLATSIAYVYSCVVLIVAMVERSDQSPVTFFDTPPMLFVFIALGRWLEHVAKSKTSEALAKLMSLQATDATVVTLGQDNLIISEEQVSVELVQRGDVVKVVPGGKFPVDGKVIEGSSMADESLITGEPMPVSKKSGSTVIAGSINAHGCLLVEATHVGADTTLCQIVKLVEDAQTSKAPIQQFADKLSGYFVPFIILVSIVTLVVWLIIGFVNFDIVAQYFPGYDKNIPRADVIVRFAFQASITVLSIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHQIKAVMFDKTGTITHGVPQVMRVLILPNTVRLSLRKILAVVGTAEASSEHPLGMAVAKYCKKELESEMLGSCTDFHTVPGCGISCKVTNVENCLQTDQQDAGVAYIEGATIDESHLFTGTNPEQSGGPAPCPAYSVLIGNREWMMRNGLHVSSDVDDAMSSHEVKGQTAILVAIDGLLCAMFAIADTVKAESVLAVHTLDSMGIQVFMITGDNRKTARAIATQVGIRKVFSEVLPSHKVAKVQELQERGWKVAMVGDGVNDSPALARADVGIAIGTGTDVAIEAADVVLIRNDLLDVVASIELSKKTVRRIRINFVFALIYNLVGIPIAAGVFMPVGLVLQPWMGSAAMAASSVSVVVSSLLLKLYKKTPVGWYEARAQGRMKSLRASEVSVHIGLDNRRCSPPTRRVWDRVSRTSRPSRSSSRSERRGSRCDTHSLLTPDRSEDFVI"

     misc_feature    251..442
                     /gene="atp7b"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(269..277,284..286)
                     /gene="atp7b"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    500..691
                     /gene="atp7b"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(518..526,533..535)
                     /gene="atp7b"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    824..1000
                     /gene="atp7b"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(842..850,857..859)
                     /gene="atp7b"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1121..1309
                     /gene="atp7b"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(1136..1144,1151..1153)
                     /gene="atp7b"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1505..1702
                     /gene="atp7b"
                     /note="Copper chaperone CopZ [Inorganic ion transport and
                     metabolism]; Region: CopZ; COG2608"
                     /db_xref="CDD:442020"
     misc_feature    1730..1921
                     /gene="atp7b"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(1748..1756,1763..1765)
                     /gene="atp7b"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1985..4120
                     /gene="atp7b"
                     /note="P-type heavy metal-transporting ATPase, similar to
                     human copper-transporting ATPases, ATP7A and ATP7B;
                     Region: P-type_ATPase_Cu-like; cd02094"
                     /db_xref="CDD:319783"
     misc_feature    order(2978..2980,2984..2986,4049..4051)
                     /gene="atp7b"
                     /note="putative Cu binding site [ion binding]; other site"
                     /db_xref="CDD:319783"
     misc_feature    order(3110..3118,3320..3322,3500..3508,3596..3598,
                     3716..3724,3782..3784,3791..3793,3800..3802,3857..3859,
                     3866..3868)
                     /gene="atp7b"
                     /note="putative ATP binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:319783"
     polyA_site      6476
                     /gene="atp7b"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

gaggaggaaagttggggacaagcacagcaactgcattgacccggctatgtacagatagagagggaaatgaactggcccgtttgcagtgacgatgtatggagctgtggggacaacaagccgggtatatcaggagggtctggagaatggcagcgccgtggggccactacagagccatgcttttcagaacctgggatatgaacccggcagccagagccagatctaccctccccccagtgacctcagccaggccaccattgacatcacgggcatgacctgccagtcctgcgtccagtccattgagggtacagtctctaaggtgaagggagtggtggccataaaggtgtctctggccaatagcaatgcccagttgcagtacaacacctccaccatcactgcacaggatgtctgccaacagatcgggaacatgggctttgatgccagagtggtgtcaagcatggctgcttctggaagcatctcctcactgatggagtcggtggtgaagattcaggtggagggcatgacctgccagtcgtgtgtgaactctattgaggggcaggtgggaaaactgcagggggtgttgcggatcagggtgtctctcggcagacaagaggctgtgattacctatcagccctgcttcattcagcctgagaacctgaggagccacattgaggagatgggcttcgaagctaccataaagaccaagccccagcctctcagactggcccccagtgaggtcgagcgcttgcagcaggataacgcccagaatgcacggagggtggataggagtggcactccgggcacagcggcagattcccacagagcgaccctggtagtggagggaatgcactgcagctcctgtgttcataacatcagggggcacctctccgagatcacaggtgtgcagagcatcacagtttccctggaggagagaagcatcgacctccagtataatccctctgtggttaccttggaatccgtgaaacgagtcatagagggactcccaccggggaacttcagagtgtctctgccctgcggaatgagcgatctgtccgctgccgcccctctgtcggtgacagacccagggttcaacgacggaggtggcctagtcaggagaacattgattaatatcaggggaatgacctgcaactcctgtgtccagtctatagaaggcatgattacccaaaggacgggtgtacgttccatacaggtctctttagccgaggagaagggaacgatactctatgaacatacactcaccagcccggatgagctgagggacaccattgaagatatgggctttgaggcatccatagcagtaaccccagaagcacctgcaacaaaacagcgcagcacccaaaaccagagctttgtgttgaagcaggactctccctgtcacaccctgccaggcactgtctcccagtctcttccccctccgcaggttcagccacaaacaccagagagagaatcccagagatcagaggaagggagaacacagaagtgcttcatacatgtgacaggcatgacatgtgcctcctgtgtggccaacatagagaggaaactgcagagacacagaggtgttgtctctgttctggtagctctaatggccgggaaagctgaggtgaagtatgacccccaggtcctgaatgctgctcagatcacccagctcatcatcagcctcggattcggagccaagctgatcgaagaccacgccatcatggatgggaaactggaccttgttgtcactggtatgacgtgtgcatcgtgtgtgcacaacattgaaacccggctgatgagcaccaaggggatactggaggcatcggtagctttggccaccagtaaagctcatatcaagtttgaccctgatgtgctgggggctcggaacataatccgcattatagaggggttggggtttggtgcagccctgatgaagaatgaaggtgtgggtaataacatggatcatcaaaaagagatacaacagtggaaaaactcgttcctgttcagcctggtgtttgggatccctgtcatggggctcatgatctatatgatcgtcatggacagcaagcacagcggctccatgcccaaagagcagaacattgtgcctggtctctccatcctcaacctagccttctttgttctctgcactcctgttcagtttctcgggggctggtacttctacgtccaagcttataaatccctgaagcacaaaacggccaacatggacgtgctcatagtgctggcgacctcgattgcatatgtttactcctgtgtggttctgatagtggccatggtggagagatcagatcaaagtccagtcaccttctttgacactcctcctatgctgtttgtgtttattgctctaggacgctggctagagcatgtagctaagagtaaaacctcagaagccctggctaagctgatgtccctacaggccacagacgcaacagtagtaacattgggacaagacaacttaattatcagtgaggaacaggtgtcagtggagctagtacagagaggagacgtcgtgaaggtggttcctggggggaagttcccagtggatggcaaagtcattgaaggcagctccatggcagatgagtctctaatcacaggggagcccatgccagtctcaaagaagagtggcagcactgtgatcgccggctccatcaacgcccatggctgtctgctggtggaggccacgcacgtcggtgctgacaccaccctgtgtcagattgtcaagctggtggaggatgcccagacctccaaggcacctatacagcagtttgcagataaactgagcggttattttgtccctttcatcatcttggtctcaattgtgaccttagtggtctggctcatcattggttttgtgaactttgacatcgtggctcaatatttccctgggtacgacaagaacatccctcgcgcagacgtcattgtgcgcttcgccttccaggcctccatcaccgtgctatccattgcctgcccctgctctctcggcctggccacacccactgctgtcatggtgggcacaggggtgggtgctcagaatggcatcctcattaaagggggggagccactggagatggcacaccagataaaggcagtgatgtttgataagactggcaccattacccatggagtgccacaggtgatgcgggtgctgattctcccaaacactgtgcgactgtccctcaggaagatactagcagtggtgggaacagcagaagccagcagtgagcaccctctgggcatggccgtggcaaagtactgcaaaaaggagctggaatctgagatgctggggtcctgcacagatttccataccgtacctggctgtgggattagctgcaaggtcaccaatgttgagaactgtctgcagacagaccagcaggatgctggtgtagcttacattgagggagccaccattgatgagagccatctgttcactggcacgaacccagaacagtcaggaggtccagccccctgtcccgcctactctgtgctgatcgggaatcgagagtggatgatgcgcaacggcctgcatgtcagtagtgatgtggatgacgccatgtccagccatgaagtaaaaggacagacagccattctggtggctatagatgggctgctgtgcgccatgtttgccattgcggacactgtgaaggcagagtcggtgctggctgtgcacaccctggacagcatgggtatccaagtgttcatgatcaccggagacaaccgcaaaactgccagagccatcgccacacaggtgggcatcaggaaggtgttttcggaggtgctgccctctcacaaggtggccaaagtgcaggagctgcaggagcggggctggaaggtggccatggtgggcgacggggtgaacgactctcctgcattggccagggctgatgtgggcatcgctatcggcacaggcaccgatgtggccatcgaggcagccgacgtggtcctgattcggaatgatctattggatgtggtggctagcattgaactatccaagaaaaccgtacgaagaatacgaatcaactttgtctttgccttgatttacaacctggtgggaatacctatcgcagcaggtgtgtttatgccagtaggcctggtgttgcagccttggatgggatcggccgcaatggctgcatcctctgtctctgtggtggtgtcttcactcctactcaaactctacaagaagaccccagttgggtggtatgaggcccgtgctcagggccgcatgaagagcctgcgtgcctctgaggtcagcgtacacatcgggctggacaaccgccgctgcagcccccccacccgcagagtgtgggaccgcgtgagtcgcaccagccgcccctcgcggtcgtcatctcgctcagagcgtaggggctcccgctgcgacacgcactccctgctcactccagacaggagcgaggatttcgtcatctgaggcgggagaggggagagaggagagccacaggtttcagccacagccggagcctcagagaacatttctgttacaatgaatggggtgtaagagtcactcctctcccctgtgtcctgaaaggaaaccataggtgccttgactttgccttccccgggactggatagcatagtctctgcctggtcattctgcatgcaccacagcttgagtaagggcacagttcatacagtggtgagggaaaagaagccctaatgatgtgaaatggaattgattgaattgggattgatagttcttgtgctttagccttgaggtgagggagaattcgaacaaggcattgcaatctccgacctggggggtatcagggaatatgacttgagaaacaatacctagtcctgttaattgaatgcagtggaaaagctaattttcatgaataaataatcataataatttcaccagcaaataactgaacccagcagttatcacagcctctggcagccactagtgggcatctgtaatgggtctcacttaagcattaagtgcattaaggaagaatgtgcttagctttctatcagatgcagtttgcactgtcagcaggtctttactagtcctctgagacagtcctctgtggtgctgagcagatagtggaggtgtgtggcctcttagcagctctgcactaatgctgcctggggctctggtgcttctttctaatctcggtttaggtggtaggtttgagcataccattcacagcaagctctcaaataagtgcactcagatgtcttgtgtatattgctgcagtgaggtatcataccctgtttccagtgaaaactcccagcaggcagatttgtgaccactggacttgtcagaccaactgagctgataacagtgtttccagtccagcttgagccacttgcttgtgagtgattctctctcagggaacctaagaagccagtatgatttatttttgacaagggaactaaaaaggggcattgcaataccatagtgtaataataataatatccacaatattgtgttgctaagaatagttctgcagttgttacaggcttattattaatgtgctgtttctgctctgccattaacaggattagcaggttgtctttaatcagtaatgaggcatggtgaaagtgtgtctatgtgtggagtaattagttcagtgcagcaggaaatgtctccaaaatcccctacccacactgtccagggtttcctctttctgggcttgtctgcatcagcctgtctctgtctcaagattgtaaccatttagcttgtgtgggaatcatttttgttccaggtacaatattctattttacataggcacaaactatcagtgtcaaatataaatacaatgatgctttataacttggttttagtcattgacaatatttaatttcattagttctagggtttgtggctatctttacaaaaaacttactttgtgcctgatttttatttttttacacctgttcatcctaaaccctacatgcacacaacatagcactgtgtgggcaaccaaaaacaaactgaatcactcacggattcaggacagccaggctggaacttgagttcagttggaaacgtggtaaaacctgacaaaccaaataaatgacaaaagagctgtttacaccagatttgctgtaaggggaaaatggagcaagctgtaaagggaacagttaagccagttggtgaactttaaccatccttttttaattttgtaattcacaaatttatcacattctcttcattcagttcatcgttcattttcagtcttattttactaatctttagcacactgtaattgtattatttaattatggtatttattttatttgaaagcccctgatatttagctataaccaattgcattatccttattatgtactctattttccactgtatgacattttaaagattttgacttttttgtattgtgtatatgtttttttttttttttattaaagtggtgtattattgtttctcagcataatcaaactggatttaatactgaaaataaagtcatgtacattgagatcaa

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