LOCUS       XM_066706392            6859 bp    mRNA    linear   VRT 30-SEP-2025
DEFINITION  PREDICTED: Amia ocellicauda ATPase copper transporting beta
            (atp7b), transcript variant X1, mRNA.
ACCESSION   XM_066706392
VERSION     XM_066706392.1
DBLINK      BioProject: PRJNA1136883
KEYWORDS    RefSeq.
SOURCE      Amia ocellicauda
  ORGANISM  Amia ocellicauda
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Holostei; Amiiformes; Amiidae; Amia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_089855) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_036373705.1-RS_2025_09
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 09/26/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Amia ocellicauda"
                     /mol_type="mRNA"
                     /isolate="fAmiCal2"
                     /db_xref="taxon:2972642"
                     /chromosome="6"
                     /sex="female"
                     /tissue_type="blood, heart, muscle, misc"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Vacherie, Louisiana"
                     /lat_lon="29.929889 N 90.747444 W"
                     /collection_date="2023-03-15"
                     /collected_by="Ingo Braasch, Allyse Ferrara"
     gene            1..6859
                     /gene="atp7b"
                     /note="ATPase copper transporting beta; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 25 Proteins"
                     /db_xref="GeneID:136751095"
     CDS             301..4818
                     /gene="atp7b"
                     /codon_start=1
                     /product="copper-transporting ATPase 2 isoform X1"
                     /protein_id="XP_066562489.1"
                     /db_xref="GeneID:136751095"
                     /translation="

MKKFSPKLINLSKYVNRSTSDASTLAGVKQVTMVVCHTNRRCESGSSSVTICDCKSEKGKIYNASRHDGEQEGLENGSAVGPLQSHAFQNLGYEPGSQSQIYPPPSDLSQATIDITGMTCQSCVQSIEGTVSKVKGVVAIKVSLANSNAQLQYNTSTITAQDVCQQIGNMGFDARVVSSMAASGSISSLMESVVKIQVEGMTCQSCVNSIEGQVGKLQGVLRIRVSLGRQEAVITYQPCFIQPENLRSHIEEMGFEATIKTKPQPLRLAPSEVERLQQDNAQNARRVDRSGTPGTAADSHRATLVVEGMHCSSCVHNIRGHLSEITGVQSITVSLEERSIDLQYNPSVVTLESVKRVIEGLPPGNFRVSLPCGMSDLSAAAPLSVTDPGFNDGGGLVRRTLINIRGMTCNSCVQSIEGMITQRTGVRSIQVSLAEEKGTILYEHTLTSPDELRDTIEDMGFEASIAVTPEAPATKQRSTQNQSFVLKQDSPCHTLPGTVSQSLPPPQVQPQTPERESQRSEEGRTQKCFIHVTGMTCASCVANIERKLQRHRGVVSVLVALMAGKAEVKYDPQVLNAAQITQLIISLGFGAKLIEDHAIMDGKLDLVVTGMTCASCVHNIETRLMSTKGILEASVALATSKAHIKFDPDVLGARNIIRIIEGLGFGAALMKNEGVGNNMDHQKEIQQWKNSFLFSLVFGIPVMGLMIYMIVMDSKHSGSMPKEQNIVPGLSILNLAFFVLCTPVQFLGGWYFYVQAYKSLKHKTANMDVLIVLATSIAYVYSCVVLIVAMVERSDQSPVTFFDTPPMLFVFIALGRWLEHVAKSKTSEALAKLMSLQATDATVVTLGQDNLIISEEQVSVELVQRGDVVKVVPGGKFPVDGKVIEGSSMADESLITGEPMPVSKKSGSTVIAGSINAHGCLLVEATHVGADTTLCQIVKLVEDAQTSKAPIQQFADKLSGYFVPFIILVSIVTLVVWLIIGFVNFDIVAQYFPGYDKNIPRADVIVRFAFQASITVLSIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHQIKAVMFDKTGTITHGVPQVMRVLILPNTVRLSLRKILAVVGTAEASSEHPLGMAVAKYCKKELESEMLGSCTDFHTVPGCGISCKVTNVENCLQTDQQDAGVAYIEGATIDESHLFTGTNPEQSGGPAPCPAYSVLIGNREWMMRNGLHVSSDVDDAMSSHEVKGQTAILVAIDGLLCAMFAIADTVKAESVLAVHTLDSMGIQVFMITGDNRKTARAIATQVGIRKVFSEVLPSHKVAKVQELQERGWKVAMVGDGVNDSPALARADVGIAIGTGTDVAIEAADVVLIRNDLLDVVASIELSKKTVRRIRINFVFALIYNLVGIPIAAGVFMPVGLVLQPWMGSAAMAASSVSVVVSSLLLKLYKKTPVGWYEARAQGRMKSLRASEVSVHIGLDNRRCSPPTRRVWDRVSRTSRPSRSSSRSERRGSRCDTHSLLTPDRSEDFVI"

     misc_feature    634..825
                     /gene="atp7b"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(652..660,667..669)
                     /gene="atp7b"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    883..1074
                     /gene="atp7b"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(901..909,916..918)
                     /gene="atp7b"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1207..1383
                     /gene="atp7b"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(1225..1233,1240..1242)
                     /gene="atp7b"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1504..1692
                     /gene="atp7b"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(1519..1527,1534..1536)
                     /gene="atp7b"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1888..2085
                     /gene="atp7b"
                     /note="Copper chaperone CopZ [Inorganic ion transport and
                     metabolism]; Region: CopZ; COG2608"
                     /db_xref="CDD:442020"
     misc_feature    2113..2304
                     /gene="atp7b"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(2131..2139,2146..2148)
                     /gene="atp7b"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    2368..4503
                     /gene="atp7b"
                     /note="P-type heavy metal-transporting ATPase, similar to
                     human copper-transporting ATPases, ATP7A and ATP7B;
                     Region: P-type_ATPase_Cu-like; cd02094"
                     /db_xref="CDD:319783"
     misc_feature    order(3361..3363,3367..3369,4432..4434)
                     /gene="atp7b"
                     /note="putative Cu binding site [ion binding]; other site"
                     /db_xref="CDD:319783"
     misc_feature    order(3493..3501,3703..3705,3883..3891,3979..3981,
                     4099..4107,4165..4167,4174..4176,4183..4185,4240..4242,
                     4249..4251)
                     /gene="atp7b"
                     /note="putative ATP binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:319783"
     polyA_site      6859
                     /gene="atp7b"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

gatgaatggcttcaccatgacctgccaggtgaccgacccacggcggtgtgtgattattcatttgcgggcaaaagttgggcttgcgtctcgagtgcaaacattgcagcacattatacaaacacaaacacaagagcgggctgtgggaagcgctgtctgcaccagtccagagtccggccagagactggtcaatcaagctgtgcgctggggttgtgacaagccgacgcaacgtgggactttactttgtgcgttaaaatatatccatatatattacgaatttgggattttaaagaaaacactgaaatgaaaaagttttcacccaagctgatcaacttatctaagtatgttaacagatctacctcggacgcttctacattagctggtgtgaagcaggtaactatggttgtctgtcacacaaaccggcgctgcgaaagcggctcctcgtctgtcacgatctgcgactgtaaatcggaaaaagggaagatttataatgccagccgacacgacggagagcaggagggtctggagaatggcagcgccgtggggccactacagagccatgcttttcagaacctgggatatgaacccggcagccagagccagatctaccctccccccagtgacctcagccaggccaccattgacatcacgggcatgacctgccagtcctgcgtccagtccattgagggtacagtctctaaggtgaagggagtggtggccataaaggtgtctctggccaatagcaatgcccagttgcagtacaacacctccaccatcactgcacaggatgtctgccaacagatcgggaacatgggctttgatgccagagtggtgtcaagcatggctgcttctggaagcatctcctcactgatggagtcggtggtgaagattcaggtggagggcatgacctgccagtcgtgtgtgaactctattgaggggcaggtgggaaaactgcagggggtgttgcggatcagggtgtctctcggcagacaagaggctgtgattacctatcagccctgcttcattcagcctgagaacctgaggagccacattgaggagatgggcttcgaagctaccataaagaccaagccccagcctctcagactggcccccagtgaggtcgagcgcttgcagcaggataacgcccagaatgcacggagggtggataggagtggcactccgggcacagcggcagattcccacagagcgaccctggtagtggagggaatgcactgcagctcctgtgttcataacatcagggggcacctctccgagatcacaggtgtgcagagcatcacagtttccctggaggagagaagcatcgacctccagtataatccctctgtggttaccttggaatccgtgaaacgagtcatagagggactcccaccggggaacttcagagtgtctctgccctgcggaatgagcgatctgtccgctgccgcccctctgtcggtgacagacccagggttcaacgacggaggtggcctagtcaggagaacattgattaatatcaggggaatgacctgcaactcctgtgtccagtctatagaaggcatgattacccaaaggacgggtgtacgttccatacaggtctctttagccgaggagaagggaacgatactctatgaacatacactcaccagcccggatgagctgagggacaccattgaagatatgggctttgaggcatccatagcagtaaccccagaagcacctgcaacaaaacagcgcagcacccaaaaccagagctttgtgttgaagcaggactctccctgtcacaccctgccaggcactgtctcccagtctcttccccctccgcaggttcagccacaaacaccagagagagaatcccagagatcagaggaagggagaacacagaagtgcttcatacatgtgacaggcatgacatgtgcctcctgtgtggccaacatagagaggaaactgcagagacacagaggtgttgtctctgttctggtagctctaatggccgggaaagctgaggtgaagtatgacccccaggtcctgaatgctgctcagatcacccagctcatcatcagcctcggattcggagccaagctgatcgaagaccacgccatcatggatgggaaactggaccttgttgtcactggtatgacgtgtgcatcgtgtgtgcacaacattgaaacccggctgatgagcaccaaggggatactggaggcatcggtagctttggccaccagtaaagctcatatcaagtttgaccctgatgtgctgggggctcggaacataatccgcattatagaggggttggggtttggtgcagccctgatgaagaatgaaggtgtgggtaataacatggatcatcaaaaagagatacaacagtggaaaaactcgttcctgttcagcctggtgtttgggatccctgtcatggggctcatgatctatatgatcgtcatggacagcaagcacagcggctccatgcccaaagagcagaacattgtgcctggtctctccatcctcaacctagccttctttgttctctgcactcctgttcagtttctcgggggctggtacttctacgtccaagcttataaatccctgaagcacaaaacggccaacatggacgtgctcatagtgctggcgacctcgattgcatatgtttactcctgtgtggttctgatagtggccatggtggagagatcagatcaaagtccagtcaccttctttgacactcctcctatgctgtttgtgtttattgctctaggacgctggctagagcatgtagctaagagtaaaacctcagaagccctggctaagctgatgtccctacaggccacagacgcaacagtagtaacattgggacaagacaacttaattatcagtgaggaacaggtgtcagtggagctagtacagagaggagacgtcgtgaaggtggttcctggggggaagttcccagtggatggcaaagtcattgaaggcagctccatggcagatgagtctctaatcacaggggagcccatgccagtctcaaagaagagtggcagcactgtgatcgccggctccatcaacgcccatggctgtctgctggtggaggccacgcacgtcggtgctgacaccaccctgtgtcagattgtcaagctggtggaggatgcccagacctccaaggcacctatacagcagtttgcagataaactgagcggttattttgtccctttcatcatcttggtctcaattgtgaccttagtggtctggctcatcattggttttgtgaactttgacatcgtggctcaatatttccctgggtacgacaagaacatccctcgcgcagacgtcattgtgcgcttcgccttccaggcctccatcaccgtgctatccattgcctgcccctgctctctcggcctggccacacccactgctgtcatggtgggcacaggggtgggtgctcagaatggcatcctcattaaagggggggagccactggagatggcacaccagataaaggcagtgatgtttgataagactggcaccattacccatggagtgccacaggtgatgcgggtgctgattctcccaaacactgtgcgactgtccctcaggaagatactagcagtggtgggaacagcagaagccagcagtgagcaccctctgggcatggccgtggcaaagtactgcaaaaaggagctggaatctgagatgctggggtcctgcacagatttccataccgtacctggctgtgggattagctgcaaggtcaccaatgttgagaactgtctgcagacagaccagcaggatgctggtgtagcttacattgagggagccaccattgatgagagccatctgttcactggcacgaacccagaacagtcaggaggtccagccccctgtcccgcctactctgtgctgatcgggaatcgagagtggatgatgcgcaacggcctgcatgtcagtagtgatgtggatgacgccatgtccagccatgaagtaaaaggacagacagccattctggtggctatagatgggctgctgtgcgccatgtttgccattgcggacactgtgaaggcagagtcggtgctggctgtgcacaccctggacagcatgggtatccaagtgttcatgatcaccggagacaaccgcaaaactgccagagccatcgccacacaggtgggcatcaggaaggtgttttcggaggtgctgccctctcacaaggtggccaaagtgcaggagctgcaggagcggggctggaaggtggccatggtgggcgacggggtgaacgactctcctgcattggccagggctgatgtgggcatcgctatcggcacaggcaccgatgtggccatcgaggcagccgacgtggtcctgattcggaatgatctattggatgtggtggctagcattgaactatccaagaaaaccgtacgaagaatacgaatcaactttgtctttgccttgatttacaacctggtgggaatacctatcgcagcaggtgtgtttatgccagtaggcctggtgttgcagccttggatgggatcggccgcaatggctgcatcctctgtctctgtggtggtgtcttcactcctactcaaactctacaagaagaccccagttgggtggtatgaggcccgtgctcagggccgcatgaagagcctgcgtgcctctgaggtcagcgtacacatcgggctggacaaccgccgctgcagcccccccacccgcagagtgtgggaccgcgtgagtcgcaccagccgcccctcgcggtcgtcatctcgctcagagcgtaggggctcccgctgcgacacgcactccctgctcactccagacaggagcgaggatttcgtcatctgaggcgggagaggggagagaggagagccacaggtttcagccacagccggagcctcagagaacatttctgttacaatgaatggggtgtaagagtcactcctctcccctgtgtcctgaaaggaaaccataggtgccttgactttgccttccccgggactggatagcatagtctctgcctggtcattctgcatgcaccacagcttgagtaagggcacagttcatacagtggtgagggaaaagaagccctaatgatgtgaaatggaattgattgaattgggattgatagttcttgtgctttagccttgaggtgagggagaattcgaacaaggcattgcaatctccgacctggggggtatcagggaatatgacttgagaaacaatacctagtcctgttaattgaatgcagtggaaaagctaattttcatgaataaataatcataataatttcaccagcaaataactgaacccagcagttatcacagcctctggcagccactagtgggcatctgtaatgggtctcacttaagcattaagtgcattaaggaagaatgtgcttagctttctatcagatgcagtttgcactgtcagcaggtctttactagtcctctgagacagtcctctgtggtgctgagcagatagtggaggtgtgtggcctcttagcagctctgcactaatgctgcctggggctctggtgcttctttctaatctcggtttaggtggtaggtttgagcataccattcacagcaagctctcaaataagtgcactcagatgtcttgtgtatattgctgcagtgaggtatcataccctgtttccagtgaaaactcccagcaggcagatttgtgaccactggacttgtcagaccaactgagctgataacagtgtttccagtccagcttgagccacttgcttgtgagtgattctctctcagggaacctaagaagccagtatgatttatttttgacaagggaactaaaaaggggcattgcaataccatagtgtaataataataatatccacaatattgtgttgctaagaatagttctgcagttgttacaggcttattattaatgtgctgtttctgctctgccattaacaggattagcaggttgtctttaatcagtaatgaggcatggtgaaagtgtgtctatgtgtggagtaattagttcagtgcagcaggaaatgtctccaaaatcccctacccacactgtccagggtttcctctttctgggcttgtctgcatcagcctgtctctgtctcaagattgtaaccatttagcttgtgtgggaatcatttttgttccaggtacaatattctattttacataggcacaaactatcagtgtcaaatataaatacaatgatgctttataacttggttttagtcattgacaatatttaatttcattagttctagggtttgtggctatctttacaaaaaacttactttgtgcctgatttttatttttttacacctgttcatcctaaaccctacatgcacacaacatagcactgtgtgggcaaccaaaaacaaactgaatcactcacggattcaggacagccaggctggaacttgagttcagttggaaacgtggtaaaacctgacaaaccaaataaatgacaaaagagctgtttacaccagatttgctgtaaggggaaaatggagcaagctgtaaagggaacagttaagccagttggtgaactttaaccatccttttttaattttgtaattcacaaatttatcacattctcttcattcagttcatcgttcattttcagtcttattttactaatctttagcacactgtaattgtattatttaattatggtatttattttatttgaaagcccctgatatttagctataaccaattgcattatccttattatgtactctattttccactgtatgacattttaaagattttgacttttttgtattgtgtatatgtttttttttttttttattaaagtggtgtattattgtttctcagcataatcaaactggatttaatactgaaaataaagtcatgtacattgagatcaa

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