LOCUS       XM_045168683            6272 bp    mRNA    linear   INV 22-NOV-2021
DEFINITION  PREDICTED: Daphnia magna protein argonaute-2 (LOC116918050),
            transcript variant X4, mRNA.
ACCESSION   XM_045168683
VERSION     XM_045168683.1
DBLINK      BioProject: PRJNA780537
KEYWORDS    RefSeq.
SOURCE      Daphnia magna
  ORGANISM  Daphnia magna
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Branchiopoda;
            Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_059183.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Daphnia magna Annotation Release 101
            Annotation Version          :: 101
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 9.0
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Daphnia magna"
                     /mol_type="mRNA"
                     /isolate="NIES"
                     /isolation_source="Duluth, Minnesota"
                     /db_xref="taxon:35525"
                     /sex="female"
                     /tissue_type="Whole Body"
                     /country="USA: Minnesota"
                     /collection_date="1986"
                     /identified_by="National Institute for Environmental
                     Studies (NIES) in Japan"
                     /linkage_group="LG2"
     gene            1..6272
                     /gene="LOC116918050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 6 ESTs, 17 Proteins, and 100%
                     coverage of the annotated genomic feature by RNAseq
                     alignments, including 138 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:116918050"
     CDS             296..3175
                     /gene="LOC116918050"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X2"
                     /protein_id="XP_045024618.1"
                     /db_xref="GeneID:116918050"
                     /translation="

MGVACTLNNTHILMDKYVNGTGALGPSGVGAQSIVSSIGVGSGSNSSSSSTSSNCGTSLSGVSGGSGGGTALMTPMAPMAPVPPMMPPTQQPPPMASAAPQAAAPELPMFVCPRRPNLGREGRPIMLRANHFQISMPRGYIHHYDISIQPDKCPRKVNREIIETMVHAYSKIFGALKPVFDGRSNLYTRDPLPIGNERVELDVTLPGEGKDRVFHVAIKWLAKVSLYALEEALEGRIRTIPMDAILALDVVMRHLPSMTYTPVGRSFFSSPDGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKSQPVIEFMCEVLDIRDVNEQRKPLTDSQRVKFTKEIKGLKIEITHCGTMRRKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAKYFLDKYKMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREREINNLIRKADFNNDPYVQEFGLTISNSMMEVRGRVLPPPKLQYGGRTKQQALPNQGVWDMRGKQFFTGVEIRVWAIACFAPQRTVREDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKSTFQGLQLVCVVLPGKTPVYAEVKRVGDTVLGMATQCVQAKNVNKTSPQTLSNLCLKINVKLGGVNSILVPTIRPKVFNEPVIFLGADITHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEVIQELSAMVRELLILFYKTTRFKPNRIIMYRDGASEGQFSTVLQHELTAIREACIKLEPDYKPGITFIVVQKRHHTRLFCADKKEQSGKSGNIPAGTTVDAGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDADELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKEHDSGEGSLQDCIKSYTSGEGSHQSGSSEDRTPGAMARAITVHADTKKVMYFA"

     misc_feature    662..1051
                     /gene="LOC116918050"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    1079..1231
                     /gene="LOC116918050"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    1232..1594
                     /gene="LOC116918050"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1388..1390,1433..1435,1475..1477,1487..1489,
                     1541..1543,1562..1564,1568..1570)
                     /gene="LOC116918050"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1727..3004
                     /gene="LOC116918050"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2138..2140,2150..2152,2186..2197,2204..2206,
                     2228..2230,2237..2239,2249..2251,2261..2263)
                     /gene="LOC116918050"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2342..2344,2348..2350,2558..2560,2972..2974)
                     /gene="LOC116918050"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      6272
                     /gene="LOC116918050"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

tcgtagctcaggcgttcctgtcattagttaaaaaaaaaaaaaaagtaaagaggagaaaagaaatttttctttccagtgcgttgtgtgtgtgtttgttttgtgtcataagagattgtgggactgatatctccaaccaagcagtgggtgtctatcgcacagttttttttcccggtcgtcgtgcgtgcgattttttggtatacggtagggcgcaactgcgcattagttggtgggggatatctcgcacacaacactttttacaggggtagtcgagtggtgcgtcttagagttaagcaaaatgggggtcgcttgtactctcaacaacactcacattcttatggataaatatgtcaatggcacgggagcacttggcccttcaggtgttggagcccaatcaattgtatccagtatcggagtgggaagtggaagcaacagtagtagcagcagtaccagtagtaattgtggaacaagtttgagtggagtgagtggtggaagtggaggcggaactgcattgatgactcccatggcccctatggctccggttccacctatgatgccaccaactcaacagcctccacctatggcttcggccgcgccgcaagctgcggcaccagaacttcctatgtttgtttgcccacgtcgtccaaatcttggtcgtgagggtagacccatcatgctaagagccaatcactttcaaatatccatgccacgaggatacatacaccactatgatatcagcatccagccagataagtgcccaagaaaagtcaatcgggagattatcgaaacgatggtgcatgcctacagcaaaatatttggagcactgaagcccgtttttgatggtcgaagcaacttatatactcgagaccctttaccgattggcaacgaacgggtcgaattagacgttactttgcctggcgagggaaaagatcgtgttttccatgtagctataaaatggttggcgaaagtatctctctacgccctcgaagaagctttagaaggtcgtattcgcacaattccgatggatgccattttggctctcgacgtggttatgcgccacttgccgtctatgacttacactccagtgggtcggagtttcttttcttcacctgatggttactaccacccactgggtgggggaagggaagtttggttcggtttccatcaatctgtccggccttctcaatggaagatgatgctaaatattgacgtttcagctacggctttttacaagtcgcaaccagtgattgaatttatgtgtgaagtcttagatattcgtgacgttaacgagcagagaaaaccattgacggattctcagcgtgtaaaatttaccaaagagatcaaaggcttgaaaattgaaattacccattgcggaacaatgcgacgaaaatatcgtgtatgcaatgtcactcgccgtccagctcaaatgcagtctttcccactgcaattggaaaatggtcaaactgtcgagtgtacagtggccaagtatttcttggacaagtacaaaatgaaattgcgctacccgcatctgccttgcctgcaggttggacaagagcacaaacatacctacttgccactggaagtttgcaacattgtagccgggcagcggtgcataaagaaactgacggacatgcaaacatcgaccatgatcaaagccactgcccgatcagctcccgaccgcgagcgtgaaattaataatctaatacgaaaggctgacttcaataacgatccctacgtccaagaatttggcttgaccatatcgaatagcatgatggaggtacgaggtcgtgtgcttcctcctcctaaactacaatatgggggaagaacaaaacaacaggcactacctaatcaaggtgtctgggatatgcgtggcaagcaattcttcactggtgttgagattcgtgtttgggcaattgcctgctttgcaccacaaagaacagtgcgagaagacgccctaaggaactttacccagcaattgcaaaaaataagcaatgatgcgggcatgcccatcattggtcaaccgtgtttctgcaagtatgccactggacccgatcaagtagagcccatgttccgctacctcaagtcaaccttccaaggtcttcagttggtgtgtgtcgtcttacccggcaaaactcctgtctacgccgaagtcaaacgcgtgggcgacacagtactgggaatggccacacagtgtgtccaagcaaaaaatgtcaacaaaacgtctcctcaaactctgtccaatttgtgtctgaagatcaacgttaaacttggtggtgtcaacagtatcctcgttcctaccattcgtccgaaggtgttcaatgaacctgtcattttccttggagctgatataacgcatcctccggccggtgacaacaaaaaaccgtcgatcgcagctgttgtgggttcgatggacgcccatccttcgcgctatgctgcgactgttcgcgtccaacaacaccgccaagaagttatccaggagcttagtgctatggttcgagaacttttgattctgttttacaaaactacaagattcaagccaaaccgaattatcatgtatcgtgatggcgccagtgaaggccagtttagtactgttttacaacacgaattgaccgctattcgcgaggcgtgcatcaaattggaacctgattacaagcccggtatcacctttatcgtagtccagaagcgtcatcacaccagacttttctgcgcggacaaaaaggaacagtcgggaaaatctggaaacattcccgccggtacgacagtcgacgctggaatcacgcatcccactgagttcgatttttacttgtgttcgcaccaaggcattcagggtactagccgccctagtcattaccacgttttgtgggatgacaaccattttgacgctgacgaattgcagtgcctgacataccaactttgtcatacttacgtgcgctgcacgcgttccgtttccattccggcgccagcttattatgcccacctggtagctttccgggcgaggtaccatttagtggaaaaagagcacgacagtggagaaggatcattgcaagattgtattaagagttacaccagtggagaaggctcgcatcagagcggcagcagcgaggatcgcactccaggtgctatggctcgcgccatcactgttcacgccgataccaagaaagtcatgtactttgcttaaaaagcactatcatcaaattcaccctccttcccagtaagttgccgtttggtcatcgcgtgtaaattggatcggatgagaatggagcagaaatggtttgtaatatttccacgtgacttgacgctgttttctctagctattttggaaggtcagcttgtgagcacaatatcttgacacctattttcatgtcacccccatcgccaccacatccctgaatcttgcgtgcaaagaggagcatttcacatccctttaacagagccatccccgacgcaaaacaaaatgtccacggccgtcaagtctttcatgtatttttcatccttatagcaaaagtcgaagcatttcggtgggatttttaatctaaattttgctcttcaaaaatatctatttgccgtctggatttctagatctggatgccgtctaaacacctaaaatccttcaaagaaaatccatcgatgcgagcaccggtcttgttgatggtcgcaaaacgaagataacgaaaaaataaaatgaaaagacgtgtgagaaaggattagggcatcaagcaaaaacggactttgtttagtagctttcgaatggataattgcatttccctcccagcgtgtggatgaagtatggaccaagcaaacgtggaatcgtatccaatcagcatttccttaatcctgacctatcgtagctacttaagagacacgacaaaatttaattgacattttcttcgttaccgatctgtttgtttatcaatccactttctttttaagaatgctccaagctatccttcctcctcttactgattaagcatctgttgcgtttcgcgggaactgaaatgacatctgaaattgaactgacgcgcgaaaaaaaaagtcaaacctaaaccttaaaaaaatagcttggatagagaaccctccgtatatgtttgttctttgatgcacttctaatcaaatttgatgaaaaaatatcgccgatccctccttccgcgctacaaacgtctttcagcctccattcgaaatgtaaaagataaagcgagcaaaacggaaagcagagagagaatgtgcatttacaatctgcaaagacagccattttgccatgacaaaaatatcgttatcccccaataatagttctttctcacaccgacatcgactactttcctaaaaaaaacaaaaaaaaaacaaacaaaagaaatgaaaaagaaaaaaaaaaaatgcgtgtttgcaagtgtgaatggcttgtgtgttgtggagtgtgagcggtcccaccacattcgggcagctcttagaacgtgagttgacgcaaatttttcaaaacagtgtttcgaggacaaaaaaaaatatattcacccacctatcggcgtctcgcgtcaacccgagaagttaattggcagtgtaatattacttcgcgaccccaatcaaagtttgaccctctcttccttctgtcttttttgtttttgttgagaatatgttttcataacatgtcttggtttctgtttgactggatcgtggaaaagcgtcttgtagcatttagttgtgtgcgttgctcttaccattttttctttttctattttggagggtagggaggaattcctgcgagcgtttaatagccatatcgtttctcggccatcgtgagaaatatcaatctttttttcttccctttttctgcgtctgtcttgttccgtctctctctcacacactgtaggttggctttggtttctctcttttgtcgcctgccgctctttgtcactgagttatgttcctcgtgtacctgtagctgctttataaacaaaagaaaatcgtgccgaattgttctctcggctactatttgctaattagtgaaactagaaggaattaaattttagacgagtcagaatactattgacatatctgatcaatttctttcgacgacaatagttttttctctaattatctcttcccgtccttgcccccccccccccaaaaaaaaaactttcctccgctcacgttcccgatccctccgacaacattttggcttttgaaaaatatgattttgggtttacttttacctctactgcatcatattccctccgcgccatttacctccaacatgtatgtatgtgcgtgagtgtatttgtaatgatttttctgttttatgttgcgcatggtgaccctttttccttctacaaaaattattattattttttttttaattttttgtttttttttccatttttgtttttctacgttgttcctctttattatcagtttcggtaggtcttttgatttcatttcagtacgggatttttttttttgttttgttttttgcttttgtttaaaaacaaaaatgcgggattcaatgagggaaaagaaactaagtgtttaaaacccttaagacaaagacaagctcttctgcccaaattttctcaactgaatccgttcaaaagtttctaaataatgtaagctcttaataatgggtagaatctgggttgctttcctggtgtttctgttttctttcaccgacattccaagtcagataggaatccaaaaaaatcaagaataaaaacaaactttaggtccctttcacgtcccgttctgaaagttccagtcagctttacttccaatcatataaacctttgcctccctttggcttgcagttaagaaaaatatagaaaaaaaaatagacgtaatagtttgatttcagtgagtaaatttatcgaacacatctcttttcgggcatatctttttttcggagagggaacgatcgtctcctctcgttctcgtttctcagctcctcagatcaatctcagttgaaaacacagaatatttgttgcgcctggaattggatctaaacaaagagaaagataatggaagacttctccgcttttatagacataaccacagaaaatggaatcatttgcgctgtcaatgttgaggggcaatcatcagtgagaataatgttatcacgtagtccttatcaaaataattctactttttttttcccctatccatctatttctgtcgtaagaatcatgcgtccatctcccgcccttgcataatttacccaattgcattatcgcaaagggggaactgtatcgtctgtaatacacttgggcgtattagcctgttttactctcaaa

//