LOCUS       XM_035680956            4590 bp    mRNA    linear   VRT 27-JUL-2020
DEFINITION  PREDICTED: Morone saxatilis argonaute RISC component 4 (ago4),
            transcript variant X6, mRNA.
ACCESSION   XM_035680956
VERSION     XM_035680956.1
DBLINK      BioProject: PRJNA647910
KEYWORDS    RefSeq.
SOURCE      Morone saxatilis (striped sea-bass)
  ORGANISM  Morone saxatilis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Moronidae; Morone.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_023340268.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Morone saxatilis Annotation Release
                                           100
            Annotation Version          :: 100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.5
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Morone saxatilis"
                     /mol_type="mRNA"
                     /isolate="NCDOM2014"
                     /db_xref="taxon:34816"
                     /chromosome="Unknown"
                     /sex="pooled male and female"
                     /tissue_type="whole blood"
                     /dev_stage="immature"
                     /geo_loc_name="USA: Aurora, NC"
                     /collection_date="2014"
                     /collected_by="Valerie N. Williams, Justin Schilling"
     gene            1..4590
                     /gene="ago4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 4 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 14 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:118342417"
     CDS             182..2773
                     /gene="ago4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X6"
                     /protein_id="XP_035536849.1"
                     /db_xref="GeneID:118342417"
                     /translation="

MEALGPGPPAPTSLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVSVVSLQMLLEALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDMSQEQLFSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA"

     misc_feature    263..652
                     /gene="ago4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    683..835
                     /gene="ago4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    836..1198
                     /gene="ago4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(992..994,1037..1039,1079..1081,1091..1093,
                     1145..1147,1166..1168,1172..1174)
                     /gene="ago4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1337..2644
                     /gene="ago4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1748..1750,1760..1762,1796..1807,1814..1816,
                     1838..1840,1847..1849,1859..1861,1871..1873)
                     /gene="ago4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1952..1954,1958..1960,2198..2200,2612..2614)
                     /gene="ago4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

cccagaaccagcagcagcagcggtagttcaggtcagtcggtccagtcagcagtcatccacgcatctatctatattcccttttcttaaattagccactttaagcttcgcgataccgcccccaatttcagcctcggtacggagccgccaggccgggacagagacggagagagaggcctcggccatggaagcgctcggacccggcccgcccgcaccaacctctctgtttcagcctccacggcgtcccggccttggcacggtgggaaaacccatccggctccttgccaaccacttccaggtgcagattcccaagattgatgtctatcactatgatatcgacatcaagcctgagaaacggcctcggagggtcaacagggaggttgtggacaccatggtgcggcacttcaagatgcaaatctttggagaccgacagcctggctacgacgggaagagaaacatgtacacagcacatccactgccgattgggagagacagggtggatctggaggtgactctgcctggcgaggggaaagatcagacgtttaaggtgtctttgcagtgggtgtctgtggtcagtcttcagatgctgctggaagccctgtcgggtcaccttaacgaggtccctgaagactccgttcaggctctggatgtcatcacgcggcacctgccttccatgaggtatactcctgtggggaggtcgtttttctcccctccggagggctattaccatcctctgggcggaggcagggaggtgtggtttggtttccatcaatctgtccgtcctgccatgtggaacatgatgctcaacatcgatgtgtcagcaacggctttctaccgcgctcagcctgtgatagagttcatgtgcgaggtgctagatatacagaacatcaacgaacagaccaaaccactgactgactcgcagcgcgtcaaattcaccaaggaaataagaggcttaaaagttgaggtcacacactgtggtcaaatgaagaggaagtaccgtgtgtgcaatgtaacgcgccggcctgcaagccaccaaacgttccccttacagcttgagaacggccaagccatggagtgcacagtagctcagtatttcaagcagaagtacaatctgcagctcaagtatccacatttaccctgcctgcaagtagggcaagaacagaagcacacctaccttcccctggaggtgtgtaatatagtagcaggccagcgctgtatcaagaaactgacagacaaccagacatccaccatgatcaaagctacagcgcgctcagcccccgacagacaggaggagatcagtagactggtcaaaagtaacagcatggtcgggggcccggacccttacctgaaggaatttggcattgttgtgcacaatgacatgacagaggtgacagggcgtgttctcccagcgcccatgctacagtatgggggccggaataagacagtggccacgcccaaccagggcgtgtgggacatgagagggaagcagttctatgctggaatcgagatcaaggtctgggctgtggcctgcttcgctccacagaaacagtgtcgagaggacctgctgaagagtttcaccgaccagcttcgaaagatctccaaggacgctgggatgcccattcaaggccagccttgtttctgtaaatacgcccaaggagctgacagtgtggagcccatgttcaaacacctcaagatgtcttatgtaggcctgcagctgattgtggtcatcctgcctggcaaaacaccagtctatgctgaggtaaagcgggtgggggacacactccttggcatggcaacccagtgcgtgcaggtgaagaacgtagtgaagacgtcccctcagaccctctccaacctgtgcctcaagatcaatgccaagctgggaggcatcaacaacgtcctcgtacctcatcagaggccctctgtgttccagcagcccgtcatcttcctgggtgcagatgtcacacatcctccagcaggtgatgggaagaagccgtccatcgcggccgtggtgggcagcatggatggccaccccagccgttactgcgccacggtgcgagtgcagacgtctagacaagacatgtcccaggagcagctctttagtcaggaggtaatccaagacctgaccaacatggtgcgggagctgctcatccagttctacaagtccacgcgcttcaagcccacacgaatcatctattatcgtggcggtgtgtcagaaggacaaatgaaacaggtagcgtggccggagctgatagccatcaggaaggcctgtatcagtctggaggaggattacaggccgggcattacctacatcgtggttcagaaacgtcaccacactcgtctcttctgctccgacaaagctgagagggttgggaagagcggtaacgtcccagcaggaaccacagtggacagcaccatcacacacccgtccgagttcgacttctacctgtgcagccatgctgggattcagggaaccagtcgtccctcccactaccacgtcctgtgggatgacaactgtttcaccgccgatgaactgcagctcctcacctaccagctgtgccacacctatgtccgctgcacgcgctccgtctccatcccagcaccggcctactacgccagactagtggctttccgagctcgctaccacctggtggacaaagatcatgacagtgctgaaggcagccacgtgtcgggtcaaagtaatggccgggacccccaggcattggccaaggcagttcagatccactatgatacccagcacaccatgtacttcgcctgagctagcgagcacgtcagccagcccacccgcggattcccttcctttctgtctttttcatctcttttttttttttttgccagtctctccctctttctctctctttttcttctcccacatctttttctctctccttgtagtctgtctctttctcttcacactcactctaattttctgaatctgcaccaaagcggctcttggacgagggagacctgctgtgtccctatgtccagcaggtttttgtttcaaggaacaatcagccaaccagcgttcccaatcgagccgcctttcaaaccagcaatccagcactgcgatccctgagggtttccagtgcacaaaagaaaagaaaaaacgacaaaaaaaacaaacaaaccatacacacatactacaagttgagccattattttgagtttgttttttctatttgaatgtctgcactcttgtgtttgtaagatacactgagcaagggagtggattggcacaatctgtgtcagcgcagctctttagctctcccagctaagcaggactcttatctacttttacctcaaagcccctttgggcaaaatctgtcacaaggtcttgggtttattgggtggtggggagggaactctacaggaaaagagatggaggggagtcgaccttttgagaatgaaaaatatatatatagatatatatatatatatatatttttttttttttcctttttatgagcgtgtgttttaactttttctcctttacggtcttttacaagcgaggtgacctgggtgtcatgcccattactactttcctctcatctaagcgaagaatacgcaagtctgtgtgcgtgtgtttgtgtgcgtgcgtgcgtgtgtgtgtgtgcgtgcatgcatctctgtatatacatgtactgctgtatgagagtttgtgtatgcatgtgtgtttcatggcaggccactgaattgtaaagggaaatcaaggagataactgctgtaaatgcctcagatttgttgtttttatattcacacatacagtacatataagcatatatttacatctatagagcaactttgagacatatgacttaaaaacaggcctccatagaggctggtgtgggtatttgtagtagtcctgggtcacttattaattgaaatgctttgaattacattttgaacgcgtatgtttgacgtgctgccagtgcagtatgggtgggatttgcacctttgggagccagctactcctttttgtgcctcaaaacatcactgaattccagaaaaaaaagtaactcaatccattttgagtagtaagtgaagtcaggtccgacgtgtagcagtagtaaccgtggtctggcctgtgactggctcagcattgggacctgttgaatggctgaaggcattaaacacagagtatggtcttatcagccttgctttctgtgtgaaaagcaggatcttatttttctttggacctgattatctgaaagattatatgatgacacggctggacgttgcttttcttcattttttttttttttttttttttgtagtagtttcaagggccggaaaccaacgtagtttttagaggtctcctttgctaatactggccaattcattgcactgacccgggacgctaggcaagcaacagagggcactctcaatctctcgtacctttatgcatttatacatacgaatcaacaaagcagatttgtaaaagtttaatataagatgttttgtggttatcttaaaaaaaaaaaaactgttgtttagaattagtagtttaccattgatgttgttgttgttatcgttgttgtcaatgttgttactattgtgatgatgaaattttacggt

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