LOCUS       XM_031719924            4959 bp    mRNA    linear   PLN 27-NOV-2019
DEFINITION  Blastomyces gilchristii SLH14081 RNA interference and silencing
            protein (BDBG_00523), mRNA.
ACCESSION   XM_031719924
VERSION     XM_031719924.1
DBLINK      BioProject: PRJNA41099
            BioSample: SAMN02953742
KEYWORDS    RefSeq.
SOURCE      Blastomyces gilchristii SLH14081
  ORGANISM  Blastomyces gilchristii SLH14081
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae;
            Blastomyces.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Champion,M., Cuomo,C., Ma,L.-J., Henn,M.R., Klein,B., Goldman,B.,
            Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L.,
            Berlin,A.M., Heiman,D.I., Hepburn,T.A., Saif,S., Shea,T.D.,
            Shenoy,N., Sykes,S., Galagan,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center.
  TITLE     The Genome Sequence of Blastomyces dermatitidis strain SLH14081
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2019) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 4959)
  AUTHORS   Champion,M., Cuomo,C., Ma,L.-J., Henn,M.R., Klein,B., Goldman,B.,
            Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L.,
            Berlin,A.M., Heiman,D.I., Hepburn,T.A., Saif,S., Shea,T.D.,
            Shenoy,N., Sykes,S., Galagan,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. This record is derived from an annotated genomic
            sequence (NW_003101682).
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Blastomyces gilchristii SLH14081"
                     /mol_type="mRNA"
                     /strain="SLH14081"
                     /db_xref="taxon:559298"
                     /chromosome="Unknown"
     gene            1..4959
                     /locus_tag="BDBG_00523"
                     /db_xref="GeneID:8507895"
     CDS             273..3401
                     /locus_tag="BDBG_00523"
                     /codon_start=1
                     /product="RNA interference and silencing protein"
                     /protein_id="XP_031575846.1"
                     /db_xref="GeneID:8507895"
                     /translation="

MSGQQPRGQRGRGGGRGMGGDPGGQRGGQRGRGSDRGRGPHPIFDAPSGGRGRGDRGGFRGDRGGRGDRGGFRGGRGGGTRGPEVYSQASGGIPAPDPKVKKVENSYKGPAGSGKELSLESLSINRNFPLRPAFGTQGRPVLLWANYFEIVSSFNLTLHRYKVEVQEQDGKTPAGKKLKRIIELLLEEHFGNMKNQIATDYKATLVCKTAINLPQEMFSITYRLEYEDEPAPRARTYKVRVVAIGSLDVAPLLQYLSSPRSGEQLPAKEEIIQALNIVLGHNPKTNRELLSIGANKHFPLGRGGESYDLSGGLTALKGFFVSVRATTSRLLVNVQVKATPCYSEGSLPDIMNSFSASIRGPTHFHKLNRFLKRVRVSVNHIVRKNKSGRVILRIKTIEGLAHQSDGRYLQNPPQVPWLGAGPKDVKFFPDGPTPPAEGQSKKNGKKQKKGPVKPGGDSSTGSYISVYDYFKRNYPNIPELNNALPVVNVGSKEEPIYLPAEVCHVLPGQPANAKISPQQTQNMIRFAVRRPVENAKSIVVNGTQVLGFTPHLNPMLAGMGLSLLPNLITVPGRVLEGPAIIQYKGQSFKSPHGGNWNLQKVAFSHGSQLPAWTFLYYQDGRTDPAVVMESVDRFIDMARVQGLAVPVPSRPIAVNVPPRRDVEDIPLDPIFAEIRQQSRVRLVLVILPFASPQIYSQVKYRGDIKDGIHTICVVAEKFRKNQPQYFANVALKFNLKLGGVNHKLQPSKLGVISEGKTMVVGIDVTHPAPGSLSTAPSIAGMVASVDKVLGQWPATIRLQQQARAEMVSDLDIMLQSRLRLWQKNNSKSLPENILIYRDGVSEGQYGKVLDEELPLLRSACKAIYPADKTKKGLPKFTIIIVGKRHNTRFYPTDLKDADNNSNPNSGTVVDRGVTESRHWDFFLQAHTALQGTARPAHYYVILDEIFTGRKTAGRFDSIADELEDLTHNLCYLFGRATKAVSVCPPAYYADLVCERARRYLSRYFDVTPDTSVASSGASDVGPVPIGDDVRIHSDLVDTMFYI"

     misc_feature    663..3395
                     /locus_tag="BDBG_00523"
                     /note="protein argonaute; Provisional; Region: PLN03202"
                     /db_xref="CDD:215631"
     misc_feature    1932..3272
                     /locus_tag="BDBG_00523"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2355..2357,2367..2369,2403..2414,2421..2423,
                     2445..2447,2454..2456,2466..2468,2478..2480)
                     /locus_tag="BDBG_00523"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2559..2561,2565..2567,2784..2786,3240..3242)
                     /locus_tag="BDBG_00523"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

gatccctacggcaacgccactggagccgctacacttagttggttacaccccaacaaaaaaacaaacaaaacatgccaagaatggcaaagagacagttggcaacgagttacataccctgcaaacttagcgctgggattgaggcctccagcaggggtcccgtcattcactggagcctcatctcaaatccacggctggtctgaaggatatacagtagtcagcttaatcccgattttttttgattgtatcattcttttccctaaccaagcagccaaatgtcaggacagcagcctcgaggtcagagagggcggggcggagggaggggaatggggggagaccccgggggtcagcgaggtggacaacgtggacgcggctctgaccgtggccgaggtccacatcctatcttcgatgcgccatcgggcggacgagggcgtggtgaccgtggcgggtttaggggagatcggggcggccgtggtgatcgtggtgggtttcgcggaggacgaggaggcggaactcgtgggccagaggtgtattcccaagctagcgggggtattcctgctccggatcccaaggtcaaaaaggtcgagaatagctataaaggacctgctggctccggaaaggaattgagtttagaatctctttcaattaacaggaatttccctctaagaccagccttcgggacacaaggaagaccagtcttactgtgggccaattatttcgaaatcgtctcaagcttcaatctcaccctgcaccgctataaggtggaagtccaggaacaggacggtaaaacccctgctggcaaaaagcttaagcgtattatcgagcttctgttggaagagcacttcggaaacatgaaaaaccaaattgcaactgactacaaagcaacactggtctgcaaaaccgcaatcaaccttccccaggagatgttttcgataacgtatcgtctcgaatatgaagatgaacctgctcccagagccaggacttacaaggttcgggtcgtcgcaattggttctctcgatgtggcaccgttgctccaatatctctcctcacccaggagcggggaacaactcccggccaaagaagagatcatccaggcattgaatatcgtcctgggccacaaccccaagacaaatcgcgaattgctttcaattggcgcaaacaaacactttccacttggccggggcggggaaagctatgatctttctggtggactcactgcactcaagggcttcttcgtcagcgtccgtgccacgacttcccgcctccttgtgaatgtccaagtcaaagctaccccgtgctacagtgaagggagcctgccagatattatgaacagcttttcagccagcatacgaggccctacgcactttcacaaactcaaccgattccttaagcgagtacgcgtgagcgtcaatcatatcgtccgcaaaaataaatcaggcagggtcatattgcgaatcaaaactattgaaggtcttgcacatcagagtgatgggcgttatttgcagaacccgccacaggtaccttggctaggagcaggtccgaaagatgtcaagttctttcctgatggaccaacacccccggcagagggccaaagcaagaagaacgggaaaaaacagaaaaagggccctgtgaagccgggtggagattcttctacgggttcatatattagcgtctacgactattttaaacgcaattaccccaatatccccgagttgaacaacgcattaccagttgtcaatgttggcagcaaggaggagccgatctacctgcctgctgaggtctgccacgttctgcctggccagccggcaaatgccaaaatctccccccaacagactcagaacatgattcgctttgcagtccggagacccgtggaaaatgccaagtcgattgtcgtaaatggaacccaggtgctcgggtttaccccacatttgaatcctatgctggctggtatgggattatctctgttgccaaacttgattactgttcctggtcgagtgctggagggtcccgctattattcagtacaagggacagagctttaaaagccctcacggcgggaactggaaccttcagaaagtcgcattctcacatgggtcccagctacctgcctggacattcctttactaccaagacggccgaaccgatccagcggttgttatggagagcgttgatagatttatagatatggcaagggtacaagggctcgctgttccagtgcctagccgtccaatcgctgtcaacgttccccctcgtcgagatgtggaggatataccactggaccccatctttgccgagattcgccagcagtcgcgagtgagattggttctggtgatcctaccttttgcaagccctcaaatttacagccaggtaaagtataggggagatatcaaggacggcatccatactatttgcgtagtcgcagaaaagttcaggaagaatcagcctcaatacttcgccaacgttgcgctaaaattcaacctgaagctcggaggagtcaaccacaaacttcagccgtccaaactgggggttatatcggaaggtaagacaatggttgtgggcattgatgtcacgcatcctgccccgggttcgctctccactgcaccaagcatagccgggatggttgcaagcgtggataaagtcctcggtcaatggcccgccaccatccgtcttcaacagcaggctcgcgcagagatggtgagtgatcttgatataatgctccaatctcgtcttcgcctttggcagaagaacaactccaagagcctcccggagaatatcctgatctaccgcgacggggtatcagaaggccaatacggcaaagtcctggacgaagaactacccctgctccgcagcgcgtgcaaagctatctacccagccgacaaaaccaagaaaggcctccccaaattcaccatcatcatcgtcggcaaacggcacaatacgcggttctacccaaccgatctcaaggacgcggacaacaactcaaatcccaacagcggaaccgtcgtcgaccgcggcgtcaccgaatcgcgccactgggatttcttcctccaagcccacaccgcactgcaggggaccgctcggccagcccactactacgtgatcctcgatgagatattcaccgggcggaagactgcaggccgcttcgacagcattgccgatgagcttgaagacctaacccataatctctgctacctgttcggacgagcaaccaaggccgtcagcgtctgcccgccggcgtactatgcggatctagtctgtgagcgcgccaggcgctacctaagtcgatatttcgacgtcacgcctgacaccagtgttgcttcgtctggtgctagtgatgtgggtcctgtgcctattggtgacgatgttcgcatacattctgacctggtggatactatgttttatatctgagtgatatctgcgtatatatcgtgtcgaagggaaggggggtatcttgggatcggggaagttgtggaaaatgctatggaggttgtgaagtcagtatggaatagtacagtcattgctctgtcattagtggtattttgaaatcttgattttgctttttagggttggttattggtctatttaccgtgatcatatctgagtatatgattgggcacatatcaaattgataagttgacacagagctttacgagaatgcaattctaaactaatgtcttggatatttctcagagagtttatctagcaaatgggtattaattgagtgatcagagttcaatcccatgcaaacctccaactactcaaatatactccgtgccaaaagctcattatcatcaccaaaatatccaccataccttcatattcatacatagagcgcacctgtcgaaatgcaaatgtacaaccttttaatcccaataaaaaaatcaaaagaaaaaaagccagctagagaaaaagagaaacctcccctccgccaatatcaccgaacaagccctactaccccccttccccccaacgaccgcacaacccccttcttcagccgaaactgcctctcaatcacctcctccgacacccctcccttatccctcatcgccaaaatatgcctaatactcacgacatccaaaaactcgcgcccggaatggctcggtcggccaacggactcgagttcgagttcggcggccttctctaaccgggcgcgggaggagagaacgaggagggctgggttggaggatgtggaggaggaggtggagggggggaagacgatggagagggattgctgaagctgcgggttttggggttggttgaaagggttgggggtgacgggcccgatggagcggagggaggcggcgaactgaggatcgcgggcttcgaggtcgatggctgtggttatttgaggccgggttcgctcgccttctcttctgcatggaatactggtcctggctgttcccgtcggcttgatgccctggccgctggatccgcggctctggcaccaccgggcccggtggtcggtgcggagggtttcttggggtattgacgccgtgcagctgcattagctgccgcctttatgggtttcgacgatgatgcgcccattgcaattttgtgtgtgcagcgttactggggagtcggagaacgatactgtacagtaactagctcggtgagcggagaatgatggatggttttgcggagacggtccaagcttctacgggggccaaagctgacaggaatgtaggtttggtggaacgctaatttgcgtctagggctagaaggtggggaggggagagcagaataaataagacacacaggtgttctaggaatcgcatgcatgggttctactaaataaataacgacctagagatacatctgttcatgaaaaaagcgctaggaagattccagctccaaagtgaagattctttgtcatatgccattttgccccgaatagcatgacgaaaaagcattagcaaaaccagaccccctagcgcagccctgcgaggcgcctctcctggacctcgct

//