LOCUS       XM_029494347            6594 bp    mRNA    linear   VRT 15-JUL-2025
DEFINITION  PREDICTED: Echeneis naucrates A kinase (PRKA) anchor protein 12a
            (akap12a), mRNA.
ACCESSION   XM_029494347
VERSION     XM_029494347.1
DBLINK      BioProject: PRJNA548465
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Echeneis naucrates (live sharksucker)
  ORGANISM  Echeneis naucrates
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangiformes; Echeneidae; Echeneis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_042532.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_900963305.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/15/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Echeneis naucrates"
                     /mol_type="mRNA"
                     /db_xref="taxon:173247"
                     /chromosome="22"
     gene            1..6594
                     /gene="akap12a"
                     /note="A kinase (PRKA) anchor protein 12a; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 4 Proteins, and 7% coverage of the annotated genomic
                     feature by RNAseq alignments"
                     /db_xref="GeneID:115036223"
     CDS             1..6594
                     /gene="akap12a"
                     /codon_start=1
                     /product="titin"
                     /protein_id="XP_029350207.1"
                     /db_xref="GeneID:115036223"
                     /translation="

MGDAQSAQREESGKAEDTEDKPFKNNKQISEINGKADSATAQVNAHCEDEMAAEDEDISKTATSLKGEVENVEMNGKEAANEADAHEDVPLEMTEMDVKQNDINEGFRRFFRNIGLKLTVKKGTAENAEMAADEMNQEEFVEVATEETKSEEHTDLKTAPETYDNDSTTCPTLTDATSEDVLQNAEEKTTDAKEVAESDIVDASPAHDDEKVQQDAALDEDQQPTSESEVVVSPIKRFFTTGIFSGLQKKKKLTEDEISEKELMEIERKHTVEEAEGCVNAQQEKEEISLGVEADPDETGQKDNKLKEEILPAASAQATDDAVSITVNEPEILSYQEKVQASPLKRLLSGSNLKKLSKKQKGRRSSDSRLSDFGDQFSDQLLSFKEPTEHQKEESPAAGEEGAWASFRKLVTPKKHPKTSAETQIPGSEEQTKPSDREQISSYSTEEGKKRKDSSVSWDAVLCGSGRRRSRSRKTSDSEDETPPAENDNKKHQSPPESSNEVDESSHKKSGSPSEADGGSTWKSFKKLVTPKRKLKDEEENRDTAQSDSEVPRDDSSFSIKKLLPGRKKSKSSEKQDQTSSDEADKDVASADEDSETPAVVPLSEFDAVETGVHIQTQAAVESHVPKEAEYELQHDLQDHTAEPLVPFDNLPTEIKKAQDEQDVLENEGSTTPTSNEEAEDLTEIISKHQQLSDIPEEGIITETMATPASAAEETARDDTLAEDLIEITSEAITAPEPVDVTLTDETEMISAVSQLSESSKTSGNTTPVPEEQDVKQTETLLHQVVETISTSPKEGLLCSDEINPERIVGSVSHQVLEMFLKEEPAILEIHRSSDATAINIGLNVEELDAANKHAVKPQRESVSEVNQSVSKESVPEVPSVRLDTAEIGVDEVHEVSVSHPEESIKELDSTDDSQTTEAVFLEALPEDEEIVADEGSVVGLHQEEIVADKGSVVQICQEEIVAEEGSVVQIPQEEEIVADEGSVVGLHQEEIVADKGSVVQICQEEIVAEEGSVVQLPQEEEIVAEEGSVVQLPQEEEIVAEEGSVVQIPQEEEIVADEGSVVRICQEEIVAEEGSVVQLPQEEEIVAEEGSVVQIPQEEEIVAEEGSVVQIPQEEEIVADEGSVVQIPQEEEIVADEGSVVGLHQEEEIVADEVSVVGLHQEEIVADEGSVVQLPQEEKPLEMDSHKAESAATQEGESKDGGVEQEVQTLTQNEDQVLQNITDQVEVEDKDKLPVEELQEAAMLNSEGGGVQLHEREIISEDSPATETHESKEETGHLTEDNVEAEREDEPQADDTEAEHVEEADVLETVRASLLDSEEGGAHLIATETGTPKEESVQLPEGEVPSKDIPTQSGTDETKEATENVSEISAEPENKELKTIALNTDNHQVAEVLQTMQEPTPASEEGGVQLLDKDEISETTAEVETVPDELKVETESLVDDDKPVDALETEHAPEIEAEAAQVTNLVSEEDGVQPPEKTDISEAIPEAETLDPTREPESNAEPEEDVPEVSLSRVNDVETEAEAEVKESMGTESGEEGRAEKPEKQISAEGDPEPDSINATASVTEEIQAELSRALEHNEEKPREITPQEDRAVQEDNATETVGELQTLAAVNGETADVQVRENVFSEMTPPTCVNNAEVTHEPEHEVHLSTVHVSIEGELPAAEVKNAVFEDALVSQVVTCYLKEASAAIPDASTAKTSENHKALIDTETSVQEFEETVMSAAPQDDDEVTESAEEGREVPTAQIEDSSSIQVQVVDVDVKSAQTIVDRVLQVGVTETKEVIDVCQETFEEVDHLSATSQTEELINEEKVTVREVIQHVKEKLPAAESLNVAMTEEAAAAAQQEVEDLTQTFVIPGSSDIICNPKEDLEETKPQLETSEADFKTEEVKLPSEELDRKKISEEAQISQMTQSQIVTHSNTGLVVPQNAGIISSVGSVDSPSSISLEFKINIQFGQAKAAVPPQPAEEKDELVKKTDVSQVEPEKEIIPPQSVDSQRQTEPTEASGQATEIRETVHSATAEKMVVMNQPILLDMGVQAIETVEPVEEIKSTERVTLNVQATETIQPVRQTEKREVFLSQPVLSEAVQETKPKEPVKPTKEENEDVWLDAEEEIYSQKETESSVERAEEPRKAESDQEEKTATPRGETAPAAKTEDEESQEEILKADATCEIESEGEDFAFALEQPETASASVSKLEED"

     misc_feature    <742..1788
                     /gene="akap12a"
                     /note="DNA topoisomerase 2-like protein; Provisional;
                     Region: PTZ00108"
                     /db_xref="CDD:240271"
     misc_feature    <2857..3384
                     /gene="akap12a"
                     /note="ribonuclease E; Reviewed; Region: rne; PRK10811"
                     /db_xref="CDD:236766"
     misc_feature    <3127..3927
                     /gene="akap12a"
                     /note="K+-dependent Na+/Ca+ exchanger; Region: 2A1904;
                     TIGR00927"
                     /db_xref="CDD:273344"
     misc_feature    <4084..4692
                     /gene="akap12a"
                     /note="Prolipoprotein diacylglyceryl transferase; Region:
                     LGT; cl00478"
                     /db_xref="CDD:469786"
ORIGIN      

atgggagacgctcagtctgcgcagcgggaggagagcggaaaagcggaggacactgaagacaagccattcaaaaataacaagcaaatctctgagatcaatggaaaagcagacagcgccacagcacaagtaaacgctcactgcgaggatgagatggctgcagaggacgaagacatttcaaaaacagcaacgtcacttaaaggggaagtggaaaatgttgaaatgaatggaaaggaagcagcaaatgaagccgatgctcatgaagacgtccctctggaaatgactgaaatggatgtgaaacagaatgatatcaatgaaggttttaggaggtttttcaggaacattggtctgaagctgacagtgaaaaagggcacggctgaaaacgctgaaatggcagcagatgagatgaaccaggaagaatttgtggaagtcgccacagaagaaaccaaaagtgaagagcatacggacctgaaaacagcgccggagacttatgacaacgattccacaacatgtcccaccctgacggatgcaacatcggaagacgttctacaaaatgcagaggagaaaacaacagacgccaaagaggtggctgaatctgatatcgtagatgcttcacctgcacatgacgatgaaaaggtgcaacaggatgccgcactcgatgaagaccaacagccaacatccgaatcagaggttgttgtgtctccaattaaaaggtttttcacaactggaatcttctctggactgcagaagaaaaagaagctcacggaagacgagataagtgagaaagagctgatggagatagagcgaaagcacacagtggaagaagcagagggatgtgtaaatgcacaacaagaaaaggaggagatcagtttgggtgttgaggcagatccagatgagacgggacagaaggacaacaaacttaaagaggagattcttcctgcagcatcagctcaggcgacagacgatgcagtaagcatcaccgtcaacgagcctgaaattctcagttatcaagagaaagttcaagccagccctctgaaaaggctcctgtcggggtctaatttaaagaaactctccaaaaagcagaaaggcaggagatcaagtgacagtaggctgtcagactttggagatcagttttcagatcagctcctgtcatttaaggagccaaccgagcatcagaaggaggagagccctgcagcaggagaggagggagcgtgggcttctttcagaaagcttgtgactccaaaaaagcacccgaagacgtccgctgagacacaaatcccaggctcagaggagcaaacgaaaccaagtgacagggagcaaatatccagttacagcacagaagaaggcaaaaaacgaaaagactcttctgtgtcgtgggacgccgttctgtgcggatctgggaggagaaggagtcgaagtaggaagacatcagactctgaggatgaaacgcctccagctgaaaatgacaacaagaagcatcagtcgccaccagaaagttccaacgaggtcgatgaaagttcccacaaaaaatcaggaagtccttcagaggctgacggaggatctacgtggaaatcctttaaaaaactggtcaccccaaaaaggaagctcaaggacgaggaggaaaacagagatactgcacagtctgacagtgaggttcctcgggatgattcctcgttctcaataaagaaacttctcccagggagaaaaaagagcaagtcctctgaaaagcaggaccaaacatcttcagatgaggctgataaggatgtagcttcagccgatgaagattcagagacacccgctgtggtcccgctgtctgagtttgatgcagttgaaaccggagttcacatacaaacgcaggctgcggtagaaagccacgtcccgaaggaagcagagtatgaactgcagcacgacctccaggatcatacggccgaacctctcgtgccttttgacaacctgccaactgagataaagaaagcccaagacgagcaggatgttttagaaaatgagggatctaccactcctacttcaaatgaagaagcagaagaccttacagaaataatcagtaaacatcaacagctcagtgatattccagaggagggcataattacagagaccatggccactccggcttcagccgccgaagagacagcacgagacgacacactcgcagaggatctgatagaaatcacgtctgaggccataactgctccagagcctgtggacgttaccctgacagacgaaactgagatgatctctgcagtttctcagctatcagagtcttcaaaaacatctggcaacacaaccccagtcccagaagaacaggatgtcaaacagacagagacgctcctgcatcaggtggttgaaaccatctccacgagcccaaaggaaggcctgctgtgctcagatgagattaaccccgaaagaatagttggatccgtctcacatcaagtgctcgaaatgtttctaaaagaggagccagcaattctggaaatacacaggagctctgatgcaactgccattaacattggcctgaatgttgaagagctggacgcagcgaataaacatgcagtaaaaccccaaagagagagtgtatctgaagtcaaccaaagtgtttccaaagaaagtgtacctgaagttccttctgtgagattggacactgctgaaattggtgtagatgaagttcatgaggtcagtgtttcacatccagaagaaagtataaaagaattagacagcacggatgacagccagacgactgaagctgtgtttttagaagctttacctgaagatgaagagattgttgcagacgaaggttctgtagtcggactccatcaagaagagattgttgcagacaaaggttctgtggtccaaatctgtcaagaagagattgttgcagaggaaggttctgtggtccaaatccctcaagaagaagagattgttgcagacgaaggttctgtggtcggactccatcaagaagagattgttgcagacaaaggttctgtggtccaaatctgtcaagaagagattgttgcagaggaaggttctgtggtccagctccctcaagaagaagagattgttgcagaggaaggttctgtggtccagctccctcaagaagaagagattgttgcagaggaaggttctgtggtccaaatccctcaagaagaagagattgttgcagacgaaggttctgtggtccgaatctgtcaagaagagattgttgcagaggaaggttctgtggtccagctccctcaagaagaagagattgttgcagaggaaggttctgtggtccaaatccctcaagaagaagagattgttgcagaggaaggttctgtggtccaaatccctcaagaagaagagattgttgcagatgaaggttctgtggtccaaatccctcaagaagaagagattgttgcagatgaaggttctgtggttggactccatcaagaagaagagattgttgcagacgaagtttctgtggtcggactccatcaagaagagattgttgcagatgaaggttctgtggtccagctccctcaagaagaaaagcctttagaaatggactctcacaaagcagaatcagctgctacacaggaaggtgaaagtaaagatggaggtgtagagcaggaagtccaaactctgacacaaaacgaagatcaagtcttacaaaacatcacagatcaagttgaagtggaggataaagataaactacctgtggaggagttacaagaagctgccatgttaaattcagaggggggaggagtccaactgcatgaaagggaaataatatccgaagacagtcctgcaactgaaacacatgaatccaaagaggaaacagggcatctcactgaagataatgttgaggcagagagggaagatgaaccccaagcagatgatactgaagctgagcacgttgaagaggcagacgttttagaaacggttcgggcatccctgttagattcagaggaaggcggtgctcatctgattgctacagaaacaggaacacccaaagaagaaagtgttcaactgcctgaaggggaagtaccatcgaaggatattcccacacaaagtgggacagatgaaacaaaagaagcaacagagaatgtctctgaaattagtgccgagccagaaaacaaagaactgaagacaatcgctctaaataccgacaatcatcaagttgcagaagttttacaaactatgcaagaacccacaccagcttcagaggagggtggtgtgcaattgcttgacaaagacgagatatcagagaccacagcagaggtagaaactgttccggatgaactcaaagtggaaactgaatctctcgttgatgacgataagccagttgatgctttagaaactgaacatgctccagaaatagaagcagaagctgcacaagtaacaaacttagtctcagaagaagacggtgtccaaccacctgaaaaaacagacatatcggaggcaattccagaggcggaaactttagatccaacacgggaacctgaaagcaacgcagagccagaagaagatgtgccagaagtttcactgtctcgtgtaaatgatgttgaaactgaagcagaggctgaagtgaaggaatctatggggacagaaagtggtgaagaggggcgtgctgagaaacctgagaagcaaatatcagctgaaggtgatcctgaaccagactctataaatgccactgcctcagttacagaggaaatacaggcagaactgagccgggctttagagcataatgaggagaagccaagagagatcacgccacaagaagatcgggctgtgcaggaagacaacgccactgaaactgtaggtgagctacaaacattagcagctgttaacggggaaacagctgatgttcaggtcagagaaaatgtcttttctgaaatgaccccgccgacttgtgtaaacaatgctgaagttacacacgaacctgagcatgaggtgcatctaagcacagttcacgtcagtattgaaggggaacttccagcagccgaggtgaagaacgctgtgtttgaggatgccttagtcagccaggtggtcacatgctatctgaaagaggcctcagctgccataccggacgcttcaacggcgaagacatcagaaaatcacaaagctttgatagacacggaaacaagtgtgcaagagtttgaggagacggtcatgtctgcggcaccgcaggatgatgatgaagtgactgagagcgcagaggaaggcagagaggtcccaacagcacagattgaggacagtagcagcattcaggtgcaggtggttgacgtcgatgtgaaatcagcccagacgattgttgaccgagtgcttcaggtcggggtgacagagaccaaagaagttatagatgtttgccaggaaacatttgaggaagtggaccatctctctgccacatcacagactgaagaattaattaacgaggaaaaggtgactgtccgagaagtcattcaacatgtgaaggaaaaattaccagcagctgaatcgctgaatgtcgccatgacagaagaggccgcggctgctgcacagcaagaggtggaggatctcacacaaacattcgttattcctggaagttcagacataatctgtaatccgaaggaggatttggaggaaactaaaccccaactggaaacctctgaagcagatttcaaaactgaagaggtgaaattaccatcagaggaattagacagaaagaaaattagtgaggaagcacaaatatcacagatgacccagagccagattgtaacccacagcaacactggattagtagtcccacaaaacgccggaataatttcctctgtaggaagtgtggattctccctctagtatttccttagaattcaaaataaacatacagtttgggcaagcaaaggcagcagttcctccacagcctgcagaagaaaaagatgaactggtaaagaagacggacgtgtctcaagttgaacctgaaaaggaaataattcccccacaaagtgtcgacagccagagacagacagagccgactgaggcgtctggtcaggcaacagaaatcagagaaacagtgcactcagccacagcagaaaaaatggtggtcatgaatcaaccgatactgctggatatgggcgtgcaggccatagaaacagtagaaccagtggaggaaatcaaatccacagagagagtgaccctcaacgtccaggcgacagagacaatacagcctgtgaggcaaacagagaagagagaggtgttcctgagccagccggtgctgtctgaagctgtccaagaaacgaaaccaaaggaacccgtgaagccaaccaaggaggaaaatgaagatgtgtggctggatgccgaggaagaaatttactctcaaaaggaaacagagtcgtcggttgaacgggcagaggaacctcgaaaggcagaaagtgaccaggaggaaaagacggcgacacctcgaggtgaaacagctcctgctgccaagactgaggacgaggaaagtcaagaagaaatcctcaaagctgacgcaacgtgcgaaattgagagtgaaggtgaagattttgcttttgcactcgagcaaccggaaactgcaagcgcaagcgtcagtaagctcgaggaggattaa

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