LOCUS       XM_023731697            3768 bp    mRNA    linear   MAM 29-JAN-2024
DEFINITION  PREDICTED: Trichechus manatus latirostris NRDE-2, necessary for RNA
            interference, domain containing (NRDE2), transcript variant X2,
            mRNA.
ACCESSION   XM_023731697
VERSION     XM_023731697.1
DBLINK      BioProject: PRJNA189960
KEYWORDS    RefSeq.
SOURCE      Trichechus manatus latirostris (Florida manatee)
  ORGANISM  Trichechus manatus latirostris
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Afrotheria; Sirenia; Trichechidae; Trichechus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_004443981.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_000243295.1-RS_2024_01
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.2
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 01/26/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Trichechus manatus latirostris"
                     /mol_type="mRNA"
                     /isolate="Lorelei"
                     /sub_species="latirostris"
                     /db_xref="taxon:127582"
                     /chromosome="Unknown"
                     /sex="female"
     gene            1..3768
                     /gene="NRDE2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 2 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 1 sample with support for all annotated introns"
                     /db_xref="GeneID:101358227"
     CDS             857..3646
                     /gene="NRDE2"
                     /codon_start=1
                     /product="nuclear exosome regulator NRDE2 isoform X2"
                     /protein_id="XP_023587465.1"
                     /db_xref="GeneID:101358227"
                     /translation="

MTFPSRSGHKDRALGSKSQSHQTHSQTENAVLKAKVRSLTGRFGRILGILSCGWRLLLFRYKRKGDSCLGINPKKQCISWEGAPTEKKNSHKHVERYFTKKNVGLMSIDGVAVSSKTEPPSSEPASFIPVKDSDDVAPSVTTWLNPLGIYDLSTTQWLQGQGPGEQDSKPPDSQPDRENAGLKAKVEEFNRKIRENPQDTQLWMAFVAFQDEVMRKPGLYAIDEGEQEKRKRSLKLILEKKLAILERAIESNQSSVDLKLAKLKLCMEFWEPATLVKEWQKLIFLHPNNTALWQKYLLFCQSQFSTFSVSKIHNLYGKCLSTLSSVKDGSILSHPVLPGTEEAMFALFLQQCHFLRQAGHSEKAISLFQAVIDFTFFQPDSVKDLPTKEQVEFFEPFWDSGEPRAGEKGARGWRAWMHQQERGGWIVVNPDEDDEEPEEDDQEIRDKTLPRWQIWLGAERSRDQRHWRPWRPDKSKKQTEEDCEDPERQVLFDDVGQSLIRLSSQDLRFQLVEAFLQFLGVPSGFVPPASCLYLAMDENNIFDPGLSEEKPLTCSTLSFSGVGRVGRMDRLGCQRWAQGHNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLRHETAKVIWCLHTKNKKKLKSQGKNCKKLAKNLLKEPENCSSLCLWKQYAHLEWLLGNTEDARQVFDTALSMAGARELKDAELCELSLLYAELEVELLSDLRGAAMSRAVHVLTRLTERSPYGPYTGQVLAVHILKARKAYERTLQDCLDESCVSDPVPTSSVNRLVSLAKCFMLFQYLTTGIDAAVRIYEQVFAKLKGSVSPAGSGLEHSGSPHGLASAMEAVTLMHTSLLRFHVKVSVYPLTPLREALSEALKWYPGNRVLWRSYVQIQSKSHSASKTRRFFDGITRSTKSLEPWLFAIEAEKMRKRLVETVQRR"

     misc_feature    <1034..1252
                     /gene="NRDE2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    1418..2416
                     /gene="NRDE2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
ORIGIN      

tgtgttccctgtcagggcagtcagtggtggtagccccaaatgaagttttatcgatggtcctttatttcaggattaaaaaaaagaatctgagacctatatgttatcttcttaagaagggatggatagaagacagcactgataggaaagtgactggtctgtgagatcgatcacctaggtcgcaggtctcagtgtccttcaccaggaacagtgtaaaagagggagtgagcatttctgccttatctgtcacctagggttgtggtgatcaaatgacataatgtgtatgtgacagtacttcaatgacaaagctgcaggagaatataaattaccgagatctggaagacaggcataatcttaaattcatgagacattaacgaagagttgaagtgttgtagaaagaagtcaaaccaggcagaaaaagttaattgcgaatcttcatatttctctaaatagaaacaatttgaaggtaaggtgaatttggatatatgcagttaacttgcataacaagaagttaagtctgatgagccttcagtcattcagaggtagtgcacgtattccctcggtaacttgttttcttcttcaaatgtaagatacaagaggaaaggagactcctgccttggcattaaccctaagaagcagtgtatatcttgggaaggggctcccacagaaaagaagaattcacacaagcatgttgaacgctattttacgaagaaaaatgtgggattgatgagcattgatggcgttgccgttagcagtaaaactgaacctccctcatcagagccggcctcatttataccagtgaaggattcggacgacgtggctccttctgtcaccacctggctgaaccctctggggatttatgacctttccatcacgcagtggtcacaaggacagggccctggggagcaagagtcaaagccaccagactcacagccagacagagaacgccgttctcaaggccaaggtgaggagtttaacaggaagattcgggagaatcctcgggatactcagctgtggatggcgtttgttacttttcagatacaagaggaaaggagactcctgccttggcattaaccctaagaagcagtgtatatcttgggaaggggctcccacagaaaagaagaattcacacaagcatgttgaacgctattttacgaagaaaaatgtgggattgatgagcattgatggcgttgccgttagcagtaaaactgaacctccctcatcagagccggcctcatttataccagtgaaggattcggacgacgtggctccttctgtcaccacctggctgaaccctctggggatttatgacctttccaccacgcagtggttacaaggacagggccctggggagcaagactcaaagccaccagactcacagccagacagagagaatgctggtctgaaggccaaggtggaggagttcaacaggaagattcgggagaatcctcaggatactcagctgtggatggcgtttgttgcttttcaggatgaggtcatgagaaagccgggcctgtatgccattgacgaaggagagcaggagaagcgaaagcggtccctgaagctcatcctggagaagaagctggccatcctggagcgggccatcgagagcaaccagagcagtgtcgacttgaaactcgccaagctgaagctctgcatggagttctgggagcccgccactctggtcaaagagtggcagaagctgatattcttgcaccccaacaatacagccctttggcagaaataccttctattttgccagagtcaatttagcaccttctctgtgtcaaaaattcacaatctttatggaaaatgcttgagtactttgtcttccgttaaggacggcagcatcttatctcaccctgtgttgcctggcaccgaggaggccatgtttgcactctttcttcagcagtgccactttctgcggcaggctggtcactcagagaaggccatctccttgttccaggctgtgattgacttcaccttcttccaacccgacagtgtgaaagatctgcctaccaaagaacaggtggaattctttgagcccttttgggacagcggtgagccccgggctggggagaagggggcccgaggctggagagcgtggatgcaccaacaggagcggggcggctggatcgttgtcaacccagatgaggatgacgaagagccagaagaggatgaccaggaaataagagataagaccctgcccagatggcagatctggctaggtgctgagcgttcccgagaccagaggcactggcgaccctggcgccctgataagagcaagaagcaaaccgaggaagactgtgaggatccagagaggcaggtgttgtttgatgacgttggacagtctctgatcagactctccagccaggatctccggtttcagctggtggaagccttcctgcagttcttgggtgtgccttctggctttgttcccccagcctcctgcctctatctggccatggatgagaacaacatctttgatcctgggctttctgaggaaaagccattgacttgttccaccctttcattctccggcgtgggccgtgtcggccgcatggaccgcttgggctgccagcgatgggcccagggtcacaaccgagagggcgaggagttcatccgcaatgtcttccacctggtgatgcctttgttttcaggcaaggaaaagtctcagctctgcttctcctggttacgacacgagactgcaaaggtcatttggtgtctgcacactaaaaacaagaagaaattaaagtcccaaggaaagaactgtaaaaaactagccaagaatctccttaaggagccagaaaactgcagcagtttgtgcctctggaagcagtatgcacacctggagtggctgctgggcaatacagaggacgccaggcaggtattcgatacagcgctcagcatggccggtgccagggagctgaaggatgccgagctctgcgagctcagtctgctatatgcagaactggaggtggagctactctcggacttgagaggggccgctatgtccagagctgtccacgtactaaccagactgaccgagaggagtccctacgggccctacaccggccaggtcttggccgttcacattctgaaggcccggaaggcttatgagcgcacactgcaggactgcttggatgagagctgtgtctcagacccagttcccaccagctccgttaaccgcctggtgagcttggccaaatgctttatgctcttccagtacttgaccacgggcattgatgctgctgtgcggatatacgagcaggtatttgcaaagctgaagggctctgtgtcccctgcaggctctggcctggagcacagcggcagcccccacggtctggccagcgccatggaggctgtcacactgatgcacacaagtttgctccggttccacgtgaaagttagcgtttaccccctgactccgctgcgagaggcactctccgaggctttgaagtggtatccgggcaaccgagttctttggaggtcgtatgttcagattcaaagcaagtcccacagtgccagcaagacccgaagattctttgatgggatcaccaggtccaccaaatccttggagccttggttgtttgcaattgaagctgagaaaatgaggaaaagactggtggaaactgttcagagaaggtagagaaatacacgccacagttcctgagagcggcttaacaaatcgaatcaaagccctgtttgaacatgcaatccagagtgacagcggcagccagtgccccttgctgtggaggatgtacttgaact

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