LOCUS       XM_020590149            6886 bp    mRNA    linear   VRT 12-JUN-2025
DEFINITION  PREDICTED: Monopterus albus argonaute RISC component 1 (ago1),
            mRNA.
ACCESSION   XM_020590149
VERSION     XM_020590149.1
DBLINK      BioProject: PRJNA380265
KEYWORDS    RefSeq.
SOURCE      Monopterus albus (swamp eel)
  ORGANISM  Monopterus albus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Anabantaria; Synbranchiformes; Synbranchidae;
            Monopterus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_018127891.1) annotated using gene prediction method: Gnomon,
            supported by mRNA evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_001952655.1-RS_2025_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/12/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Monopterus albus"
                     /mol_type="mRNA"
                     /db_xref="taxon:43700"
                     /chromosome="Unknown"
                     /sex="male"
                     /geo_loc_name="China"
     gene            1..6886
                     /gene="ago1"
                     /note="argonaute RISC component 1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 1
                     mRNA, 38 Proteins, and 97% coverage of the annotated
                     genomic feature by RNAseq alignments, including 4 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:109954662"
     CDS             280..2826
                     /gene="ago1"
                     /codon_start=1
                     /product="protein argonaute-1"
                     /protein_id="XP_020445805.1"
                     /db_xref="GeneID:109954662"
                     /translation="

MGVFPPPLQQVFHAPRRPGMGTVGKPIKLLANYFEVEIPKMDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQLFGDRKPVYDGKKNIYTVLALPIGSEKVDFEVTIPGEGKDRIFKVSIRWLAKVSWRLLQETLVSGRLQVPLDSVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKTLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNANFNLDPYIQEFGIKVKDDMAEVTGRVLPAPILQYGGRSRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKSERIGKSGNIPAGTTVDTSITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHVSGQSNGRDPQALAKAVQIHHDTLRTMYFA"

     misc_feature    352..744
                     /gene="ago1"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    772..924
                     /gene="ago1"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    925..1287
                     /gene="ago1"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1081..1083,1126..1128,1168..1170,1180..1182,
                     1234..1236,1255..1257,1261..1263)
                     /gene="ago1"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1420..2697
                     /gene="ago1"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1831..1833,1843..1845,1879..1890,1897..1899,
                     1921..1923,1930..1932,1942..1944,1954..1956)
                     /gene="ago1"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2035..2037,2041..2043,2251..2253,2665..2667)
                     /gene="ago1"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

cctatgcctacgcttccttcctgacttgtgcccacattttcctctcttttcgtcctgtcctctgctgttcacaaccacttcctttgccacatcctcacttaactgttgtttttctcttctggacctactttgtccatgtccttccgctgctttggcttgtccttgttgttgctttgtgcacatccaatttgctcattcctgttttctatgctttgaacttccctttcgccattacctgtctttgtcaatggcccctcacctcctctgtgtccagtgcccatgggggtcttccccccacctctgcagcaggtgttccatgccccgcgccggccaggcatgggtactgtgggcaagcccatcaagctgctggccaactatttcgaggtggagatcccaaagatggacgtctaccactacgaggtggacattaagcctgacaagtgcccacgacgagtcaacagggaggtggtggaatacatggtgcaacacttcaagccccagctctttggtgataggaagccagtgtatgacggcaagaagaacatctacacggtgctagcacttcctattgggagtgagaaggtggattttgaggtaacaattccaggtgagggtaaggaccgaatcttcaaggtgtccatccgttggctggccaaggtgtcgtggcgtctgctgcaggagactctggtcagcggccgcttgcaggtccccctcgactcggttcaagccctggatgtggccatgcgccacctggcctctatgaggtacactccagtgggccgttcatttttctccccacctgaaggatactaccacccactggggggcgggagggaagtctggtttggcttccaccagtctgtgcggcccgccatgtggaagatgatgcttaacattgatgtgtctgccacagccttttacaaagcccaacctgtaattgagttcatgtgcgaggttttggacattcgcaacatcgatgagcagcccaagactctcactgactcacaaagggtccgcttcaccaaggagattaaaggcctgaaggtggaggtgacccactgtggtcaaatgaagaggaaatatcgtgtatgtaatgtcacccgacgtcctgccagccaccagactttccccctgcagcttgaaagtgggcagacagtagaatgtacagtggcccagtacttcaagcagaagtacaacctgcagctaaagtacccccatctaccctgtctacaggtgggccaggagcagaagcacacctacctgcccctggaggtgtgtaacattgtagcaggccaacgatgcattaagaagctgacagataatcagacctccactatgatcaaagccacagctcgctctgcgcccgacagacaagaggagatcagcaggctgatgaagaatgctaacttcaacctggacccgtacatccaggagtttggtatcaaagtcaaagatgacatggctgaggtgacaggcagggtgctccccgcccctatcctgcagtatggaggacggagccgtgccatagctacccccaaccagggagtgtgggacatgagggggaagcagttttataatggcattgagatcaaagtctgggcaattgcctgctttgctccccagaagcagtgcagagaagaggtgctcaagaacttcacagaccagctgcgtaagatctctaaggatgctgggatgccaattcagggccagccatgcttctgtaaatatgcccagggagcagacagcgtggagcccatgttcagacacctgaagaacacatattctggactacagctcatcattgtcatcctaccaggaaaaactcctgtatatgcggaggtaaagcgtgtaggtgatactctccttggcatggccacccagtgtgttcaggtgaaaaatgtagtgaaaacgtcacctcagaccctctccaacctctgcctcaagatcaacgtgaagctgggaggcatcaacaacatcctggtgcctcaccaacggtcagcagtgtttcaacagccggttatcttcctgggagcagatgtcacgcacccccctgccggagatggcaagaagccctccattactgctgtggtaggcagtatggatgctcatccgagcagatattgtgccacagtgcgagtccagcggcccaggcaggagatcattgaagatttgtcttacatggtgcgcgaactgctaattcagttctacaagtcaactcgattcaagcccaccaggatcattttttacagagatggagttcctgagggtcagttgccgcagattctccactatgagctcctggccatcagagatgcatgcatcaagctggagaaagactatcagccgggcatcacctacatcgtggtgcagaaacgccaccacacacgcctcttctgtgctgacaagtctgaaaggattgggaagagtgggaacattcctgcagggactacagtggacaccagcatcactcatccctttgaatttgacttctacctgtgtagccatgcaggaatacagggaaccagccggccgtctcattactatgtcctttgggacgacaatcgcttcacggctgatgagctgcagattctgacataccagttgtgccacacttatgtgcgttgtacacgctcagtctccatccctgctccagcctactatgctcgtcttgtggctttccgtgcccgctaccatctggtggacaaagaacacgacagtggagagggcagccatgtctctggtcagagtaatggtcgggacccccaggcactggccaaagctgtacagattcaccatgacaccctcaggaccatgtactttgcctgagctacaccaaccatcacagccattgacccctcccacctcatttctacctgagtgggctgatgctcaccacctcatccccatcccttattcccaatgtctgcccactacacatactccgcagccttgccagccacccaagtccaagaaacacaaggccctttcacctgtaaaacacagccagactctgcagcctcatgcattcaaaaggttatgaatattccatccatccattaattttcagctgcttatccagggttgggttgcagtggcagcaggttcagcggggcagtccagatgtccctctccccagcaacgttttccagctcctgctgggggactttgagccattcccaggttggatgagatgtataatctctctagcatattctaccccagggcctcccagcagatggatgtgcccagaaaaccatcagtgaaaggtgcccaggaggcatcctgatcagatgcctgaagcacttcacctggctccttttgatgtgaagcatctgtgactctactccaagccccctctaggataaacaaactccataccctatctctaaggctgatcctagccacatgacagaggaaagtcattgtgactgtgtgtgtttctgatctcattctttcggtcactacccaaagctcatgaccataggtgagagctggaatgtagactgactagtaaattggaagcttttccttccagctcagctcccccttcaccacaacggtctggtacagtacccacattactgctgccactgcgctaatccaccagtcactctcatgctccattttagccccactaatttataagaccctgagatacttagtcttttgcatgggcaagcaaatcacctgcaactcaaaaggagcagcccaccattttccagtagaaaaccatagcctcaacttggaggcgctacttcacacttggctccaaaccaccccattgcatgctaagggcacagtctgatgaaccacgtcatctgcaaaaggcagagaagcgattctgaggcccccagaccgaacacattactccccctggttgtgtcttgagatcctggtgatgatcagttgacacctctgctcctctcttcacctcatatagccacaaatgtgatgatacaaacacaaagctgatcattgatcttacacctaagatgctctagtacaagtacacatatttggacatcttatgttcaaacatggcgtttatggccgatctagtccaatatcaacacaccatttgggttcagatcagacaggcagttactcccaggcacaccccaccaggtttctccattgttgcacatgttaacattgaaatcccccaacaaaactatggagtccctaggtggtggtggtgccctttccaggaccacaaccagagtctccaagaagactgaatactttgaactgctgtttggggcataaacgcagacaaaagtcagggcctacccctgaggatcgtaaagctgcatggaggaaccttgttctccacagaaaactcaaagacagcagcactcaggcagaggctcgtgagtatcctcacacctgcccagcacctctcaccctggacaactccagaaaagaatagggtccagttctactatatcaagttggtttctctccacctcctgcaccagctctagctccttcccctgtcagacaggtgatggttatgaatattattattactgttattgtcatcttgtccctcagagaattgccacctatgcggagggaatgtttatcctgttgatcctggaaactgtgttgttgggggcagtatcccttggtagggtctcaaggaaaattggtccctggggaggggccagacaaagagattccaagaacaccgatgagtcagactttttggagctatgtcacctcacccaagggaaaacaggaccccttcctgaagccaaaccttggagggaggtgaccgcatggatcaagggcccatgagtcatggcccatggggattactcagcatggggcaaagcttgaaaaaaattatatggagccactaccttgtgggtccaccacctacagggataggaattggggtcgggtgcaatgtaagctgggtggcaggcaaggttggggacctgggcatgctggtccctggtgttgtagatggttatgaatattattattgtcattttacgtttactttaagacaattgctctttttatgttgaatttttaaaaatgtgggtattattatgtattgtattgtatgctatcttcacactcctgtgtatgcaatacggatgggaggaagatagacgttaaaaagaaaaaaaatccagaaaacttttttaactaccaccacgatgctgcctcttgacccatccgaacaccggatgccagtgttccaagcccctttaatatggggatcctacagaggggtggtactgggcacacacacttacagtacatgtatcatttttaacatcatgaactacagtcatgtttttaatgtttttgaataatgttatttttttatacagccttgaggattctaaggtatggtacattagcacttagtgttagcatgtaggtgccaccagtcctgcacataagagtttgaacaggtgtagcatatcatagatacaggagagaagtcccactgcactctttgtggtggttggtcagtcccttagagatttagcctatagaggcaaagcctaacaatagagggaccatttgcatagagtgtatcaaatatacttgcaccccatgcacctaaaccagcattattaaccagccgaaagtgatgccaaccaatggaaaaggggcacttaaaacaggaagatagtcttttatttctgtatggattgctcttttaggatatacaaggcacttttttttttacttattgatttagtttatttcaaaatggtgaaacttgtctgtactgaggcttgacacacagactaccttgacgttttcacacccacacttgcacagctttcagaacaaagaggtaactaaggcaataggcaaggtcaacactcctctgtagatttgaatacaggcaactatgaagtctgtgatcacttaagtgctgcaaagctgaactctcattcagcaactgaccttgctccaaccattctgcacattccagcatgtatcagagttgacaccaacttccacccatgcacttctttgcatacctatacacatctatctggcacctgttccatttttacatgaagctcttccctctgcaggcatttacaacttatagcctggaaagcagaagcacacacatctgcaccctcttagtctctttctaaacatgtagagacccactttttcaaatgcacttgtcctcttatttccctccaagccaaactataactcatcaccatttccatcttcactttctgtcattgctgtctattcttacttttaactggcagcatcccctgccccaatcccctagcttatgttagccagtccccattggtgctctgtgtgcatgggccgcacaggtaggggactgttacattggatagggtgggccactattactactacttaatatgccctgcttttacatcttgtcactttcttgagtaatgaaaaaatgcatgtaattattttttatgactggatattctgagccaccttatttaggttttgttagaggtaggagccttgtgctcaccactgcctgtcagtcgttttgcctcgattgtgtaatgttataaagactgaattttttttcgtttgtcattaatatgaatattgtgactagtgtcaagagtgtatcctcaagaaggcactgtagagactgtctcaaaattccacctcagttgcccgtttaagcgcctcaaatcttgtctgacggtcaggtcccctctgagatgaaagggatcaaaagattagacatcagatgagtttgatttttaaataatgtctatgaacttgcataatcaaaatgcctttgtacaccgttttactgatccttcattgtgtccaaggtgatttgtatatataaatatataatattatcctgttatgtattttggctcaccagttg

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