LOCUS       XM_018879960             963 bp    mRNA    linear   PLN 07-JAN-2024
DEFINITION  Sugiyamaella lignohabitans 3-dehydrosphinganine reductase (TSC10),
            partial mRNA.
ACCESSION   XM_018879960
VERSION     XM_018879960.1
DBLINK      BioProject: PRJNA342695
            BioSample: SAMN04417247
KEYWORDS    RefSeq.
SOURCE      Sugiyamaella lignohabitans
  ORGANISM  Sugiyamaella lignohabitans
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Trichomonascaceae;
            Sugiyamaella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Bellasio,M., Peymann,A., Valli,M., Sipitzky,M., Graf,A., Sauer,M.,
            Marx,H. and Mattanovich,D.
  TITLE     Complete genome sequence and transcriptome regulation of the
            pentose utilising yeast Sugiyamaella lignohabitans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 963)
  AUTHORS   Peymann,A. and Graf,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2016) Department of Biotechnology, University of
            Natural Resources and Life Sciences, Muthgasse 18, Vienna, Vienna
            1190, Austria
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. This record is derived from an annotated genomic
            sequence (NC_031674).
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Sugiyamaella lignohabitans"
                     /mol_type="mRNA"
                     /strain="CBS 10342"
                     /type_material="culture from holotype of Candida
                     lignohabitans"
                     /db_xref="taxon:796027"
                     /chromosome="B"
     gene            <1..>963
                     /gene="TSC10"
                     /locus_tag="AWJ20_2977"
                     /db_xref="GeneID:30034947"
     CDS             1..963
                     /gene="TSC10"
                     /locus_tag="AWJ20_2977"
                     /inference="similar to AA sequence:KEGG_Orthology:K04708"
                     /note="3-ketosphinganine reductase; catalyzes the second
                     step in phytosphingosine synthesis; essential for growth
                     in the absence of exogenous dihydrosphingosine or
                     phytosphingosine; localized to lipid droplets; member of
                     short chain dehydrogenase/reductase protein family;
                     GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
                     11914276]; GO_component: GO:0005783 - endoplasmic
                     reticulum [Evidence IEA]; GO_component: GO:0005783 -
                     endoplasmic reticulum [Evidence IDA] [PMID 11914276];
                     GO_component: GO:0005783 - endoplasmic reticulum [Evidence
                     IDA] [PMID 14562095]; GO_component: GO:0005789 -
                     endoplasmic reticulum membrane [Evidence IEA];
                     GO_component: GO:0016021 - integral component of membrane
                     [Evidence IEA]; GO_component: GO:0005811 - lipid particle
                     [Evidence IDA] [PMID 24868093]; GO_component: GO:0016020 -
                     membrane [Evidence IEA]; GO_component: GO:0005741 -
                     mitochondrial outer membrane [Evidence IDA] [PMID
                     16407407]; GO_function: GO:0047560 - 3-dehydrosphinganine
                     reductase activity [Evidence IEA]; GO_function: GO:0047560
                     - 3-dehydrosphinganine reductase activity [Evidence IDA]
                     [PMID 9804843]; GO_function: GO:0016491 - oxidoreductase
                     activity [Evidence IEA,IEA]; GO_process: GO:0006666 -
                     3-keto-sphinganine metabolic process [Evidence IDA] [PMID
                     9804843]; GO_process: GO:0006629 - lipid metabolic process
                     [Evidence IEA]; GO_process: GO:0008152 - metabolic process
                     [Evidence IEA]; GO_process: GO:0055114 -
                     oxidation-reduction process [Evidence IEA]; GO_process:
                     GO:0030148 - sphingolipid biosynthetic process [Evidence
                     IDA,IMP,ISS] [PMID 9804843]; GO_process: GO:0006665 -
                     sphingolipid metabolic process [Evidence IEA,IEA]"
                     /codon_start=1
                     /product="3-dehydrosphinganine reductase"
                     /protein_id="XP_018737827.1"
                     /db_xref="GeneID:30034947"
                     /translation="

MSYFETKGKLAVISGGSQGLGAALAKQLILKGSNVVIVSRTESKLQKTVTSLELSRLNDDQLIKYIVADVSDPTECSRVFQELDDVPDIVICCAGGAVPGLLVDMEADTLKKHMAICYDTALFFSHAAMKVMIPKQKEIEAHHGRPSKRHLIYCSSVLALFPFIGYGSYGPAKAAIRALADIARQECIPYNISVANILPGNMATEGFVEEEKTKPEITRKIEGPSDARDPDIVAKEVIRDLERGQEMIYTDLIGWILSGLMMGASPRNWGIFQSIIAAVLVFFAPVWMFFVRKDIKDYFQKKTEEEHISKTKTEQTSNSK"

     misc_feature    22..762
                     /gene="TSC10"
                     /locus_tag="AWJ20_2977"
                     /note="3-ketodihydrosphingosine reductase (KDSR) and
                     related proteins, classical (c) SDR; Region:
                     KDSR-like_SDR_c; cd08939"
                     /db_xref="CDD:187643"
     misc_feature    order(43..45,49..60,115..123,202..210,277..285,346..348,
                     460..468,505..507,517..519,595..606,610..615)
                     /gene="TSC10"
                     /locus_tag="AWJ20_2977"
                     /note="putative NAD(P) binding site [chemical binding];
                     other site"
                     /db_xref="CDD:187643"
     misc_feature    order(217..219,301..309,313..315,328..330,340..342,
                     364..366,376..378,385..390,484..495,499..501,508..513,
                     520..525,529..537,541..549,553..558,577..579,694..696,
                     706..708,718..720,724..729,745..762)
                     /gene="TSC10"
                     /locus_tag="AWJ20_2977"
                     /note="homotetramer interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:187643"
     misc_feature    order(349..351,466..468,505..507,517..519)
                     /gene="TSC10"
                     /locus_tag="AWJ20_2977"
                     /note="active site"
                     /db_xref="CDD:187643"
     misc_feature    order(571..573,577..579,694..696,703..708,718..720,
                     724..726,745..762)
                     /gene="TSC10"
                     /locus_tag="AWJ20_2977"
                     /note="homodimer interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:187643"
ORIGIN      

atgtcttattttgaaaccaaagggaagctggcagtaatatcaggtggttcacagggactgggggctgcactggcaaaacagcttatactcaaggggtctaatgttgttatcgtttcgagaactgaatcaaagctacagaaaactgtcacttcactagaattatccagattgaacgatgatcaattgatcaaatatattgtagcagatgtctcagatcctacagaatgttcccgtgtattccaggagctcgacgatgttccagatattgttatttgttgtgctggcggtgcggtcccaggattgctggtagacatggaagctgataccctgaagaaacacatggcaatttgttatgataccgcactgttcttttctcatgcagccatgaaggtcatgattcctaaacaaaaggaaatagaggcccatcacggacgtccctcaaaacgacatttaatctactgctcatctgtcttggcattgttcccttttattggatatggatcctatgggccagctaaagctgctataagagctctggctgatatagctcgtcaggaatgtattccttataacatatcggttgcgaatattttacctggaaatatggcaaccgagggttttgttgaggaggagaagactaagccagaaattacacgtaaaatcgagggtccttcggatgcacgggatcctgatattgtagctaaagaagtcattcgtgatctggaacgtggtcaagaaatgatatatacggatttaattggctggattctcagtggtctgatgatgggtgctagtcccagaaattggggcattttccaatctatcattgctgcagtgctagtgttttttgcacctgtgtggatgttcttcgtgcgaaaagatattaaggactattttcagaagaagacggaagaggaacatattagtaaaactaaaactgagcagacttcgaattctaaatag

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