LOCUS       XM_010590475            3074 bp    mRNA    linear   MAM 05-APR-2024
DEFINITION  PREDICTED: Loxodonta africana nuclear factor kappa B subunit 1
            (NFKB1), transcript variant X3, mRNA.
ACCESSION   XM_010590475
VERSION     XM_010590475.3
DBLINK      BioProject: PRJNA1092696
KEYWORDS    RefSeq.
SOURCE      Loxodonta africana (African savanna elephant)
  ORGANISM  Loxodonta africana
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Afrotheria; Proboscidea; Elephantidae;
            Loxodonta.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_087346) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Apr 5, 2024 this sequence version replaced XM_010590475.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_030014295.1-RS_2024_04
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.2
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 04/02/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Loxodonta africana"
                     /mol_type="mRNA"
                     /isolate="mLoxAfr1"
                     /db_xref="taxon:9785"
                     /chromosome="5"
                     /sex="male"
                     /cell_line="Cultured endothelial cells from umbilical
                     cord; passage 7"
                     /tissue_type="Original tissue source of cells is from
                     umbilical cord"
                     /dev_stage="juvenile"
                     /geo_loc_name="USA: Louisville Zoo, Kentucky"
                     /lat_lon="38.2057 N 85.7071 W"
                     /collection_date="2022-02-22"
                     /collected_by="Austin Bow"
     gene            1..3074
                     /gene="NFKB1"
                     /note="nuclear factor kappa B subunit 1; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 11 Proteins"
                     /db_xref="GeneID:100662194"
     CDS             115..2997
                     /gene="NFKB1"
                     /codon_start=1
                     /product="nuclear factor NF-kappa-B p105 subunit isoform
                     X3"
                     /protein_id="XP_010588777.1"
                     /db_xref="GeneID:100662194"
                     /translation="

MFHLEQLNHTIFNPELFQPEVPLPTADGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHPDLAYLQAEGGGDRQLTDREKEIIRQAALHQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGTGSTGPGYGFPPYGFPTYGGITFHSGTTKSNAGMKHGAIGTPSKKDHEGCDKSDDRDVINLSGKVTETTEQDKKSSNGHDGVTLTYTVGVKEENCRFQDNLFLEKAMQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTAGLISNDIINMRNDLYQTPLHLAVITKQEDVVEDLLRAGADLSLLDRLGNSVLHLAAKEGHDKILSVLLKHKQAALLINQPNGEGLNAIHIAMMSNSMSCLRLLVAAGADVDAQEQKSGRTALHLAVEHDNISLAGCLLLEGDALVDSTTFDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKDGEDEGVVPGTTPLDMAANWQVFDILNGKPYESEFTSDDLLAQGDMKQLTEDAKLQLYKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTVKELVEALRQMGYTEAIEVIQTAFYSSGTAATSSEKTPSQAHSLPLSPSSTRQQIDELRDNESICDSGVETSFRKLSFTESLTSSSSLLTLNKMPHNYGQQGSIEGNV"

     misc_feature    199..804
                     /gene="NFKB1"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD) of nuclear factor of kappa B1 (NF-kappa B1); Region:
                     RHD-n_NFkB1; cd07935"
                     /db_xref="CDD:143651"
     misc_feature    order(241..243,247..252,256..261,268..279,502..504,
                     508..513,802..804)
                     /gene="NFKB1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143651"
     misc_feature    823..1128
                     /gene="NFKB1"
                     /note="IPT domain of the transcription factor NFkappaB and
                     related transcription factors. NFkappaB is considered a
                     central regulator of stress responses, activated by
                     different stressful conditions, including physical stress,
                     oxidative stress, and exposure to...; Region:
                     IPT_NFkappaB; cd01177"
                     /db_xref="CDD:238582"
     misc_feature    order(826..828,832..840,844..852,970..978,1015..1017,
                     1051..1053,1108..1110,1117..1119,1123..1125)
                     /gene="NFKB1"
                     /note="ankyrin protein binding site [polypeptide binding];
                     other site"
                     /db_xref="CDD:238582"
     misc_feature    order(832..837,841..843,880..882,886..888,892..894,
                     991..996,1003..1005,1009..1011)
                     /gene="NFKB1"
                     /note="dimerization interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:238582"
     misc_feature    order(895..897,901..903,994..999)
                     /gene="NFKB1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238582"
     misc_feature    1552..2373
                     /gene="NFKB1"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     Region: ANKYR; COG0666"
                     /db_xref="CDD:440430"
     misc_feature    order(1690..1692,1696..1698,1708..1713,1720..1728,
                     1732..1737,1747..1749,1756..1758,1801..1803,1807..1809,
                     1813..1815,1825..1830,1837..1845,1849..1854,1864..1866,
                     1873..1875,1900..1902,1906..1908,1912..1914,1924..1929,
                     1936..1944,1948..1953,1963..1965,1972..1974)
                     /gene="NFKB1"
                     /note="oligomer interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:293786"
     misc_feature    1690..1803
                     /gene="NFKB1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    1807..1902
                     /gene="NFKB1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    2014..2106
                     /gene="NFKB1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    2116..2214
                     /gene="NFKB1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    2512..2733
                     /gene="NFKB1"
                     /note="Death domain of the Nuclear Factor-KappaB1
                     precursor protein p105; Region: Death_NFkB1_p105; cd08797"
                     /db_xref="CDD:260063"
     polyA_site      3074
                     /gene="NFKB1"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

cccgcacccggacagcttcgactccggcctcttcgtccccgagcccccaggcccgcgctgccccggacggggagcccacaggcgcagtgaggccagcgcagactcgcgaccacgatgtttcacttggaacaattgaatcatacgatatttaatccagaattatttcaaccagaggtgccactgccaacagcagatggcccgtaccttcaaatattagagcaacctaaacagagaggattccgtttccgttatgtatgtgaaggcccgtcccatggtggacttcctggtgcgtctagtgaaaagaacaagaagtcctaccctcaggtcaaaatctgcaactatgtgggacctgcaaaggttattgttcagctggtcacgaatggaaaaaacatccacctgcatgcacacagcctggtggggaagcactgtgaggacgggatctgcactgtgactgctggacccaaggacatggtggtcggctttgcaaacctgggtatacttcatgtgacaaagaaaaaagtatttgagacactggaagcacgaatgacagaggcgtgtataagaggctataaccctggacttttagtgcatcctgatcttgcctatttgcaagcagaaggtggaggagacaggcaactcacagatcgggaaaaggagataatccgccaggctgctcttcatcagacaaaagagatggacctcagcgtggtgcggctcatgtttacagcttttcttccggacagcacgggcagcttcaccaggcgcctggagcccgtggtctcagatgccatctacgacagcaaagcccccaacgcgtccaatttgaaaattgtaagaatggacaggacagccggatgtgtaactggaggggaagagatttatcttctctgtgacaaggttcagaaagatgacatacagattcgattttatgaagaggaggaaaatggtggagtctgggaaggatttggagatttttcccccacagatgttcataggcagtttgccatcgtcttcaaaaccccaaagtataaagatgtcaacattacaaaaccagcctctgtgttcgtccaacttcgaaggaaatctgatttggaaactagtgaaccaaaaccttttctctactaccctgaaatcaaagataaagaggaagtgcagaggaaacggcagaagctcatgcccaatttttcggatagtttcggtggtggtagtggtgctggagctggaggtggaggcatgtttggtagtggcggcggaggagggggtactggaagtactggcccagggtatggcttcccaccctatggatttcctacatatggtggaatcaccttccattctggaaccactaaatctaatgctgggatgaagcacggagccataggcaccccatctaaaaaagaccatgaaggttgtgacaaaagtgatgacagagatgttataaatctttctgggaaagtaactgaaaccacagagcaagataagaagtccagcaatgggcatgacggggttactctgacgtatacagtaggagtaaaggaagagaattgcaggtttcaggataacctctttctagagaaggctatgcagcttgccaagcggcacgccaatgccctttttgactatgcggtaacaggagacgtgaagatgctgctggcggtccagcgccatcttactgcagtgcaggatgagaatggggacagcgtcttgcacttagcaatcatccaccttcatgcacagctggtgagggatctgctagaagtcacagcgggtttgatttccaatgacatcatcaacatgagaaatgatctttatcagacacccttgcacttagcagtgattaccaagcaagaagatgtggtggaggacttgctgagggccggggctgacctgagtctcctggaccgcctgggtaactctgttttacacctagctgccaaagaaggacatgataaaattctcagtgtcttactcaagcacaaacaggcagcactacttatcaaccagcctaacggggaaggtttgaacgccattcacatagccatgatgagcaacagcatgtcctgtctgcggctgctggtggccgcaggcgcggacgtcgatgctcaggagcagaagtctgggcgcacggcactgcacctggctgtggagcacgacaacatctccttggctggctgcctgctcctggagggtgatgcccttgtggacagtaccacctttgatggaactacgcccttgcatatagcagccgggagagggtccaccaggctggcagctcttctcaaagcagcaggagcagatcccctggtggagaactttgagcctctctatgacctggatgactcttgggaaaaggatggagaggacgaaggggtcgtgcctggaactacacctttagatatggctgccaactggcaggtttttgacatactaaatggaaaaccatatgagtcagagtttacatctgatgatttactggcacaaggagacatgaaacagctgactgaagatgcaaagttgcagctctacaagttgctagaaattcctgatccagacaaaaactgggccactctggcacagaaattaggtctggggatacttaataatgccttccgactgagtcctgctccttctaaaactcttatggacaactatgaggtctctggaggaacagtaaaggaactggtggaggccctgaggcagatgggctacacggaagcaattgaagtcatccagacagccttctacagctcagggacggcagccaccagctcagagaagacaccctctcaggcccactcgctgcctctctcaccttcctctacaaggcagcaaatagatgagctccgagacaacgaaagcatctgtgacagcggcgtggagacctctttccgcaaactcagcttcacagagtctctgaccagcagcagctcattgctaactctgaacaaaatgccccacaattatggacagcagggatccatagaaggcaatgtttaacctgctggtgattttcaacactgtgcaaaaccaaagtcctgaagttccactgcattgtccaaaagaagtaaggtaaa

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