LOCUS       NM_001336183            1867 bp    mRNA    linear   PLN 20-OCT-2022
DEFINITION  Arabidopsis thaliana 3-ketoacyl-CoA synthase 12 (KCS12), mRNA.
ACCESSION   NM_001336183
VERSION     NM_001336183.1
DBLINK      BioProject: PRJNA116
            BioSample: SAMN03081427
KEYWORDS    RefSeq.
SOURCE      Arabidopsis thaliana (thale cress)
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Camelineae; Arabidopsis.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Lin,X., Kaul,S., Rounsley,S., Shea,T.P., Benito,M.I., Town,C.D.,
            Fujii,C.Y., Mason,T., Bowman,C.L., Barnstead,M., Feldblyum,T.V.,
            Buell,C.R., Ketchum,K.A., Lee,J., Ronning,C.M., Koo,H.L.,
            Moffat,K.S., Cronin,L.A., Shen,M., Pai,G., Van Aken,S., Umayam,L.,
            Tallon,L.J., Gill,J.E., Adams,M.D., Carrera,A.J., Creasy,T.H.,
            Goodman,H.M., Somerville,C.R., Copenhaver,G.P., Preuss,D.,
            Nierman,W.C., White,O., Eisen,J.A., Salzberg,S.L., Fraser,C.M. and
            Venter,J.C.
  TITLE     Sequence and analysis of chromosome 2 of the plant Arabidopsis
            thaliana
  JOURNAL   Nature 402 (6763), 761-768 (1999)
   PUBMED   10617197
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 1867)
  AUTHORS   Krishnakumar,V., Cheng,C.-Y., Chan,A.P., Schobel,S., Kim,M.,
            Ferlanti,E.S., Belyaeva,I., Rosen,B.D., Micklem,G., Miller,J.R.,
            Vaughn,M. and Town,C.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Plant Genomics, J. Craig Venter Institute,
            9704 Medical Center Dr, Rockville, MD 20850, USA
  REMARK    Protein update by submitter
REFERENCE   4  (bases 1 to 1867)
  AUTHORS   Swarbreck,D., Lamesch,P., Wilks,C. and Huala,E.
  CONSRTM   TAIR
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2011) Department of Plant Biology, Carnegie
            Institution, 260 Panama Street, Stanford, CA, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by TAIR and Araport.
            This record is derived from an annotated genomic sequence
            (NC_003071).
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Arabidopsis thaliana"
                     /mol_type="mRNA"
                     /db_xref="taxon:3702"
                     /chromosome="2"
                     /ecotype="Columbia"
     gene            1..1867
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="Encodes KCS12, a member of the 3-ketoacyl-CoA
                     synthase family involved in the biosynthesis of VLCFA
                     (very long chain fatty acids)."
                     /db_xref="Araport:AT2G28630"
                     /db_xref="GeneID:817412"
                     /db_xref="TAIR:AT2G28630"
     CDS             97..1527
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /inference="Similar to RNA sequence,
                     EST:INSD:AV793974.1,INSD:EL292259.1,INSD:EL059716.1,
                     INSD:EL050145.1,INSD:EL225259.1,INSD:EL329552.1,
                     INSD:EH977518.1,INSD:CB258919.1,INSD:ES148844.1,
                     INSD:DR273098.1,INSD:EL137938.1,INSD:BX839645.1,
                     INSD:EL292217.1,INSD:EH822380.1,INSD:EL253018.1,
                     INSD:DR273099.1,INSD:EL294535.1,INSD:EL152957.1,
                     INSD:EL132441.1,INSD:EL056683.1,INSD:EL038329.1,
                     INSD:EL220567.1,INSD:BP642141.1,INSD:BP804352.1,
                     INSD:DR379886.1,INSD:EL143764.1,INSD:AV816395.1,
                     INSD:EL296674.1,INSD:BP589113.1,INSD:DR273100.1,
                     INSD:EL190602.1,INSD:N96274.1,INSD:EL335087.1,
                     INSD:BX835713.1,INSD:EL334559.1,INSD:BP588616.1,
                     INSD:EL008752.1,INSD:EL299133.1,INSD:EH814541.1,
                     INSD:BP859748.1,INSD:EL028340.1,INSD:EH799456.1,
                     INSD:EL272244.1,INSD:EH844689.1,INSD:EL302587.1,
                     INSD:BP790391.1,INSD:EG528835.1,INSD:EH856254.1,
                     INSD:BP617872.1,INSD:BP857478.1,INSD:EL044894.1,
                     INSD:BP626039.1,INSD:BP851900.1,INSD:EL026138.1,
                     INSD:EL031983.1,INSD:DR273101.1,INSD:BP812723.1,
                     INSD:BP671809.1,INSD:ES006932.1,INSD:EL003881.1,
                     INSD:EL093046.1,INSD:EL000527.1,INSD:EH958802.1,
                     INSD:EL983798.1,INSD:EH896509.1,INSD:EG528834.1,
                     INSD:EH798002.1,INSD:EL060398.1,INSD:BP861093.1,
                     INSD:BP783558.1,INSD:BP665849.1,INSD:BP621978.1,
                     INSD:EL269966.1,INSD:ES040901.1,INSD:AV826202.1,
                     INSD:EH940292.1,INSD:EL209303.1,INSD:CF772916.1"
                     /inference="similar to RNA sequence,
                     mRNA:INSD:AY084716.1,INSD:BX820690.1,INSD:AY056170.1,
                     INSD:BX820515.1,INSD:AY091195.1"
                     /note="3-ketoacyl-CoA synthase 12 (KCS12); FUNCTIONS IN:
                     transferase activity, transferring acyl groups other than
                     amino-acyl groups, catalytic activity, acyltransferase
                     activity; INVOLVED IN: response to osmotic stress; LOCATED
                     IN: endoplasmic reticulum; EXPRESSED IN: 24 plant
                     structures; EXPRESSED DURING: 13 growth stages; CONTAINS
                     InterPro DOMAIN/s: Thiolase-like (InterPro:IPR016039),
                     Very-long-chain 3-ketoacyl-CoA synthase
                     (InterPro:IPR012392), 3-Oxoacyl-[acyl-carrier-protein
                     (ACP)] synthase III C-terminal (InterPro:IPR013747),
                     FAE1/Type III polyketide synthase-like protein
                     (InterPro:IPR013601), Thiolase-like, subgroup
                     (InterPro:IPR016038); BEST Arabidopsis thaliana protein
                     match is: 3-ketoacyl-CoA synthase 3 (TAIR:AT1G07720.1);
                     Has 2195 Blast hits to 2173 proteins in 528 species:
                     Archae - 0; Bacteria - 1011; Metazoa - 0; Fungi - 4;
                     Plants - 1037; Viruses - 0; Other Eukaryotes - 143
                     (source: NCBI BLink)."
                     /codon_start=1
                     /product="3-ketoacyl-CoA synthase 12"
                     /protein_id="NP_001318305.1"
                     /db_xref="Araport:AT2G28630"
                     /db_xref="GeneID:817412"
                     /db_xref="TAIR:AT2G28630"
                     /translation="

MDLLFLFFSLLLSYLFFKIWKLIDSKQDKDCYILDYQCHKPTDDRMVSTQFSGEIIYRNQNLGLTEYKFLLKAIVSSGIGEQTYAPRLVFEGREERPSLQDGISEMEEFYVDSIGKLLERNQISPKDIDILVVNVSMLSSTPSLASRIINHYKMRDDVKVFNLTGMGCSASLISVDIVKNIFKSYANKLALVATSESLSPNWYSGNNRSMILANCLFRSGGCAILLTNKRSLRKKAMFKLKCMVRTHHGAREESYNCCIQAEDEQGRVGFYLGKNLPKAATRAFVENLKVITPKILPVTELIRFMLKLLIKKIKIRQNPSKGSTNLPPGTPLKAGINFKTGIEHFCIHTGGKAVIDGIGHSLDLNEYDIEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKRGDRVFMISFGAGFKCNSCVWEVVRDLTGGESKGNVWNHCIDDYPPKSILNPYLEKFGWIQDEDPDTFKVPDAFM"

     misc_feature    163..1011
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="FAE1/Type III polyketide synthase-like protein;
                     Region: FAE1_CUT1_RppA; pfam08392"
                     /db_xref="CDD:429970"
     misc_feature    order(250..252,259..264,745..747,964..966,1147..1155)
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="malonyl-CoA binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:238427"
     misc_feature    order(379..384,391..393,514..519,532..534,544..546,
                     562..582,592..597,622..627,634..636,643..651,856..858,
                     862..870,904..915,1342..1344)
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="dimer interface [polypeptide binding]; other site"
                     /db_xref="CDD:238427"
     misc_feature    order(598..600,1138..1140,1237..1239)
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="active site"
                     /db_xref="CDD:238427"
     misc_feature    order(682..690,748..750,898..906,961..966,1243..1245,
                     1333..1335)
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="product binding site [active]"
                     /db_xref="CDD:238427"
     misc_feature    1093..1365
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="Beta-ketoacyl synthase, C-terminal domain; Region:
                     Ketoacyl-synt_C; cl21486"
                     /db_xref="CDD:451266"
ORIGIN      

atgcatcttactcacttcatcatctcttcttaaaccccaaaacaaacaaacaaacaaacaaaaaataccaaaacaaagaagaaaaaaaaaataactatggatcttctctttctctttttctctcttctcctctcttacctctttttcaagatctggaaactcatagactcaaagcaagacaaagattgctacattttagactaccaatgtcataaaccaaccgacgatcgaatggtgagcactcaattcagtggagagatcatttatcgaaaccaaaaccttggtttaaccgaatacaagttccttctcaaagccatagtaagctcagggattggagaacaaacctacgctccaagacttgtctttgaaggtcgagaagagcgtccttcgttacaagatgggatctccgagatggaagagttctacgtggacagcatcggaaaactcctggagagaaatcaaatatctcctaaagacatagacatcctcgtagtcaacgtctccatgctctcttcaacgccatctttagcttcaagaatcattaaccattacaagatgagagacgacgtcaaagtcttcaacttgaccgggatgggatgcagcgcaagtctaatctccgtagacattgtcaagaacatttttaagagctacgcaaacaagttagctcttgttgccacctccgagtctttgagccctaattggtatagcggaaacaaccgttcaatgatcttagccaactgtttgttccggtccggtggatgtgctattctcttgactaacaaacgtagcttgagaaagaaagcaatgtttaaactcaagtgtatggtacggactcaccatggagctagagaggagtcttataattgttgcatccaagccgaagatgaacaaggccgtgtcgggttttacttggggaagaatctaccaaaagccgctactcgtgcttttgtagaaaacctcaaggttataacacccaagattcttcccgtaaccgagcttataaggttcatgttaaagcttcttatcaagaaaatcaagattcgtcaaaaccctagcaagggctccacgaatctcccaccggggactccattaaaggcaggaatcaacttcaagaccgggatcgaacatttttgcatacataccggaggaaaagctgtgattgatgggattggacatagcttggatttaaacgagtatgatattgaacctgcgaggatgactcttcaccggtttggcaatacttcggcgagtagtttgtggtatgtgttggcttacatggaggccaagaagagattgaagagaggagatagagtttttatgataagctttggagctggttttaagtgtaatagctgcgtttgggaagttgtgagagatcttactggtggtgaatcaaaaggaaatgtgtggaatcattgcattgatgattatccaccaaaatcgattctgaatccttatttggagaagtttggctggattcaagatgaagatcctgacactttcaaggtccctgacgctttcatgtaaaacgtttacgcacaaaaacgcaaacgcaaacgcaaaaacaacacaaggatcgaattagttaaaacgtgtttagcaatcggaaaacctaataaatgcgtataactatacatcattatgtcagactgttcatggttttttatgccatcactttcatacggagaagacctagttggagacgttattatcgttcactagattatgagaaagcatcttctcaaatcaaaattggttctaatctgtttatttttgatgagtcaaatgtatcgaaatatccttttcttgagtgccattgttacaaggtttcaataacgttactcaatttaggtttagctaataaagc

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