LOCUS       NM_001183937            1305 bp    mRNA    linear   PLN 15-SEP-2023
DEFINITION  Saccharomyces cerevisiae S288C ribonuclease T2 (RNY1), partial
            mRNA.
ACCESSION   NM_001183937
VERSION     NM_001183937.1
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Bussey,H., Storms,R.K., Ahmed,A., Albermann,K., Allen,E.,
            Ansorge,W., Araujo,R., Aparicio,A., Barrell,B., Badcock,K.,
            Benes,V., Botstein,D., Bowman,S., Bruckner,M., Carpenter,J.,
            Cherry,J.M., Chung,E., Churcher,C., Coster,F., Davis,K.,
            Davis,R.W., Dietrich,F.S., Delius,H., DiPaolo,T., Hani,J. et al.
  TITLE     The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI
  JOURNAL   Nature 387 (6632 SUPPL), 103-105 (1997)
   PUBMED   9169875
REFERENCE   3  (bases 1 to 1305)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   4  (bases 1 to 1305)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2023) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 1305)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2015) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 1305)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   7  (bases 1 to 1305)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001148).
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="XVI"
     gene            <1..>1305
                     /gene="RNY1"
                     /locus_tag="YPL123C"
                     /db_xref="GeneID:855980"
     CDS             1..1305
                     /gene="RNY1"
                     /locus_tag="YPL123C"
                     /EC_number="4.6.1.19"
                     /experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
                     vacuole [PMID:24102742]"
                     /experiment="EXISTENCE:direct assay:GO:0004521 RNA
                     endonuclease activity [PMID:11158587]"
                     /experiment="EXISTENCE:direct assay:GO:0005576
                     extracellular region [PMID:11158587]"
                     /experiment="EXISTENCE:direct assay:GO:0005773 vacuole
                     [PMID:19332891]"
                     /experiment="EXISTENCE:direct assay:GO:0005829 cytosol
                     [PMID:19332891|PMID:26928762]"
                     /experiment="EXISTENCE:genetic interaction:GO:0006915
                     apoptotic process [PMID:19332891]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0000902 cell
                     morphogenesis [PMID:11158587]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0004521 RNA
                     endonuclease activity [PMID:19332891]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006401 RNA
                     catabolic process [PMID:19332891]"
                     /note="Vacuolar RNase of the T(2) family; relocalizes to
                     the cytosol where it cleaves tRNAs upon oxidative or
                     stationary phase stress; required for tRNA-specific
                     translational pausing during oxidative stress; promotes
                     apoptosis under stress conditions and this function is
                     independent of Rny1p catalytic activity"
                     /codon_start=1
                     /product="ribonuclease T2"
                     /protein_id="NP_015202.1"
                     /db_xref="GeneID:855980"
                     /db_xref="SGD:S000006044"
                     /translation="

MLLKNLHSLLQLPIFSNGADKGIEPNCPINIPLSCSNKTDIDNSCCFEYPGGIFLQTQFWNYFPSKNDLNETELVKELGPLDSFTIHGLWPDNCHGGYQQFCNRSLQIDDVYYLLHDKKFNNNDTSLQISGEKLLEYLDLYWKSNNGNHESLWIHEFNKHGTCISTIRPECYTEWGANSVDRKRAVYDYFRITYNLFKKLDTFSTLEKNNIVPSVDNSYSLEQIEAALSKEFEGKKVFIGCDRHNSLNEVWYYNHLKGSLLSEMFVPMDSLAIRTNCKKDGIKFFPKGYVPTFRRRPNKGARYRGVVRLSNINNGDQMQGFLIKNGHWMSQGTPANYELIKSPYGNYYLRTNQGFCDIISSSSNELVCKFRNIKDAGQFDFDPTKGGDGYIGYSGNYNWGGDTYPRRRNQSPIFSVDDEQNSKKYKFKLKFIKN"

     misc_feature    148..852
                     /gene="RNY1"
                     /locus_tag="YPL123C"
                     /note="Ribonuclease T2 (RNase T2) is a widespread family
                     of secreted RNases found in every organism examined thus
                     far. This family includes RNase Rh, RNase MC1, RNase LE,
                     and self-incompatibility RNases (S-RNases). Plant T2
                     RNases are expressed during leaf...; Region: RNase_T2_euk;
                     cd01061"
                     /db_xref="CDD:238512"
     misc_feature    order(160..162,268..270,751..753)
                     /gene="RNY1"
                     /locus_tag="YPL123C"
                     /note="B1 nucleotide binding pocket [chemical binding];
                     other site"
                     /db_xref="CDD:238512"
     misc_feature    order(172..174,187..189,454..456,745..747)
                     /gene="RNY1"
                     /locus_tag="YPL123C"
                     /note="B2 nucleotide binding pocket [chemical binding];
                     other site"
                     /db_xref="CDD:238512"
     misc_feature    order(250..273,454..489)
                     /gene="RNY1"
                     /locus_tag="YPL123C"
                     /note="CAS motifs [active]"
                     /db_xref="CDD:238512"
     misc_feature    order(259..261,268..270,274..276,463..468,475..480)
                     /gene="RNY1"
                     /locus_tag="YPL123C"
                     /note="active site"
                     /db_xref="CDD:238512"
ORIGIN      

atgttactgaaaaacttacacagtctcttacaactaccaattttttcgaatggagcagataagggtatagaaccaaactgccctataaacattccattatcatgttccaataaaactgatatagacaactcgtgttgttttgaatatccaggtggaatatttttacaaacccaattctggaattactttccaagcaaaaacgatttaaatgaaactgaattagtgaaggagttagggcctctagattcatttacaattcacggattatggccagataattgtcatggtggctaccaacaattctgtaataggtccttacaaattgacgatgtttactacttattgcatgacaagaaatttaataataatgatacatccctgcaaatatcgggcgaaaagctgcttgaatacctagacttatattggaagagtaataacgggaatcatgagtccttatggatacacgagtttaataaacatggcacgtgcattagcacaattagaccagagtgctatactgagtggggtgctaatagtgttgacagaaaaagagcggtctatgattattttagaataacttataatctattcaagaaattggacacattttcaacactagaaaaaaataatattgtcccaagtgtggacaattcctattctttggagcagatagaggcagcactaagtaaagagtttgaaggaaaaaaagtcttcataggctgtgatagacataattccttaaacgaagtatggtattataaccacttgaagggttcccttttgagcgaaatgtttgtgcccatggactcacttgccattcgaacaaattgtaaaaaagatggtattaagttttttccaaaaggttatgtcccaactttcaggaggagacctaataagggagcaagatacagaggagtcgttcgtctatcaaatattaataatggagatcagatgcaaggctttctaatcaagaatggacactggatgagtcaaggtacaccagcgaattacgagttgattaaatctccctatgggaattactacttgagaactaaccaagggttttgtgacattatttcgtcttcatctaatgaattggtctgcaaattcaggaacattaaggatgcaggtcaattcgattttgatccaacgaaaggaggagacggatatattggttattctggtaactacaactggggcggtgacacctatccaaggagaaggaatcaaagccccattttctctgtagacgatgaacaaaattccaagaaatataagtttaaattaaaattcatcaaaaattaa

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