LOCUS       NM_001183505            3561 bp    mRNA    linear   PLN 17-DEC-2024
DEFINITION  Saccharomyces cerevisiae S288C tricalbin (TCB1), partial mRNA.
ACCESSION   NM_001183505
VERSION     NM_001183505.1
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Dujon,B., Albermann,K., Aldea,M., Alexandraki,D., Ansorge,W.,
            Arino,J., Benes,V., Bohn,C., Bolotin-Fukuhara,M., Bordonne,R.,
            Boyer,J., Camasses,A., Casamayor,A., Casas,C., Cheret,G.,
            Cziepluch,C., Daignan-Fornier,B., Dang,D.V., de Haan,M., Delius,H.,
            Durand,P., Fairhead,C., Feldmann,H., Gaillon,L., Kleine,K. et al.
  TITLE     The nucleotide sequence of Saccharomyces cerevisiae chromosome XV
  JOURNAL   Nature 387 (6632 SUPPL), 98-102 (1997)
   PUBMED   9169874
REFERENCE   3  (bases 1 to 3561)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   4  (bases 1 to 3561)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 3561)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2015) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 3561)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   7  (bases 1 to 3561)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   8  (bases 1 to 3561)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2010) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   9  (bases 1 to 3561)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001147).
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="XV"
     gene            <1..>3561
                     /gene="TCB1"
                     /locus_tag="YOR086C"
                     /db_xref="GeneID:854253"
     CDS             1..3561
                     /gene="TCB1"
                     /locus_tag="YOR086C"
                     /experiment="EXISTENCE:direct assay:GO:0005739
                     mitochondrion [PMID:14576278|PMID:16823961]"
                     /experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
                     reticulum [PMID:26928762]"
                     /experiment="EXISTENCE:direct assay:GO:0005886 plasma
                     membrane [PMID:14562095]"
                     /experiment="EXISTENCE:direct assay:GO:0008289 lipid
                     binding [PMID:15049706]"
                     /experiment="EXISTENCE:direct assay:GO:0032541 cortical
                     endoplasmic reticulum [PMID:22250200]"
                     /experiment="EXISTENCE:direct assay:GO:0071944 cell
                     periphery [PMID:26928762]"
                     /experiment="EXISTENCE:genetic interaction:GO:0055091
                     phospholipid homeostasis [PMID:35440494]"
                     /experiment="EXISTENCE:genetic interaction:GO:0060304
                     regulation of phosphatidylinositol dephosphorylation
                     [PMID:23237950]"
                     /experiment="EXISTENCE:genetic interaction:GO:0090158
                     endoplasmic reticulum membrane organization
                     [PMID:23237950]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0035621 ER to
                     Golgi ceramide transport [PMID:33205016]"
                     /note="Lipid-binding ER tricalbin involved in ER-plasma
                     membrane tethering; one of at least 7 proteins (Ice2p,
                     Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering
                     and contact with the plasma membrane (PM); regulates PI4P
                     levels by controlling access of the Sac1p phosphatase to
                     its substrate PI4P in the PM; regulates PM phospholipid
                     homeostasis; contains 3 calcium and lipid binding domains;
                     localizes to the cortical ER and to mitochondria;
                     C-termini of Tcb1p, Tcb2p and Tcb3p interact"
                     /codon_start=1
                     /product="tricalbin"
                     /protein_id="NP_014729.1"
                     /db_xref="GeneID:854253"
                     /db_xref="SGD:S000005612"
                     /translation="

MAKEDTGVTAPKKPETAQVANINGIDKLEPPKTKEETESSKSVSSEKAAHASDESFKRSIHEASYVGWKQIGGWEDKDELTLDDELMDMTRETFLDNIIPDSLYGDWYHSVAIFFIGGVASFALGHYKFSMGSAFFVIVITSLLYRTSAKKYRGSIRELVQKEFTVQKVENDYESLEWLNAFLDKYWPILEPSVSQLIVQQANEQMATNEAIPKFITQLWIDELTLGVKPPRVDLVKTFQNTASDVVVMDWGISFTPHDLCDMSAKQVRNYVNELAVVKAKIFGITIPVSVSDIAFKAHARVKFKLMTPFPHVETVNIQLLKVPDFDFVATLFGRSIFNWEILAIPGLMTLIQKMAKKYMGPILLPPFSLQLNIPQLLSGSNLSIGILEITVKNAKGLKRTSSILNESIDPYLSFEFNDISIAKTRTVRDTLNPVWDETLYVLLNSFTDPLTISVYDKRAKLKDKVLGRIQYNLNTLHDKTTQRNLKAQFLRNSKPVGELTFDLRFFPTLEEKKLPDGSVEELPDLNTGIAKVVVEEGSRFAEEEQKVTAYVEVYLNAKLVLTTGKATDTGTLKWNSDYEAVIADRRKTRYKFVVKDGKGEEIGSTIQTLNDLIDRSQVNKNLIPLKNQKGDIKITTYWRPVRLEIGSNSVAYTPPIGAIRVFIEKANDLRNLEKFGTIDPYCKVLVNGLSKGRTDFKSQTLNPVWNQVIYVAVTSPNQRITLQCMDVETVNKDRSLGEFNVNVQDLFKKDENDKYEETIDEKAKVGRLVMPKKKPKGTITYYTSFYPALPVLTLEEIQDLDKVNKKKKALELRKSAIDEKKISKEDKAKFDQEWNEVKELEDMYSNRQKLDLPELLQYNQGVLAVTVLNGELPDSGLYVQAFFDDNGHPRFVSPRIPSRIVKNGWSGDVIIKELDKSITTFRVAKNKNYNRVEKCVCEVELPTQELVKNCYYKPSILHLSGEGSAKLMLQISWFPIDTKQLPANDLITNSGDLTIMSRSAENLIASDLNGYSDPYLKYYINNEEDCAYKTKVVKKTLNPKWNDEGTIQINNRLNDVLRIKVMDWDSTSADDTIGTAEIPLNKVKVEGTTELDVPVEGLENAGQDGGMLHLAFSFKPRYTISVSKREKKVGDIASKGLGTGLKAGTTVIGGGVGAIGKIKKGVFGGLGSLTNHKKNHEMGEEETKF"

     misc_feature    1..3546
                     /gene="TCB1"
                     /locus_tag="YOR086C"
                     /note="Ca2+-dependent lipid-binding protein, contains C2
                     domain [General function prediction only]; Region:
                     COG5038"
                     /db_xref="CDD:227371"
ORIGIN      

atggccaaagaagatactggggtaacagcaccaaagaagcccgaaacagcccaagtagctaacataaatgggatagataagttagaacctccaaagacgaaagaggaaaccgaatccagtaaaagcgtgtcatctgagaaggcggcacatgctagtgatgaaagcttcaagagaagtatccatgaagcttcttatgtcggctggaaacagattggtggctgggaagacaaagacgaattaactttagatgatgaattgatggatatgactagagaaaccttcttagataacatcataccggacagcctttacggtgactggtatcattcggtagccattttctttatcggaggcgtggcatcatttgcacttggtcattataagttttcaatggggtcggccttttttgtcatcgttatcacttcattgttgtatagaacctctgctaaaaaatacagaggttccataagagagttagtccagaaagagttcacggtgcagaaagtggaaaacgactatgaatccctagaatggttgaatgcctttttggataagtactggcccattttagaaccttccgttagtcaattaatcgttcagcaagctaatgaacaaatggctactaatgaggcgattcccaaatttattactcaactatggatcgatgagttgacacttggtgttaaaccccccagagttgatttggtgaagactttccaaaacactgcatccgatgttgttgtgatggactggggtatttcttttactcctcacgatttatgcgacatgagtgctaagcaagtcagaaattacgttaatgaattagcggttgttaaggccaaaatttttggtatcaccatccctgtttctgtgtcagacattgctttcaaggctcatgctagagtgaaattcaaattgatgactcccttccctcatgtcgaaactgttaatatccagctactgaaggttcctgattttgattttgttgctaccttattcggccgttccatcttcaattgggaaattttagctatcccaggccttatgacactaatacaaaagatggccaagaaatatatgggtccaatcttgttacctccattttctttacaattgaacattccacaactcttatctggttccaacttgtccattggtatattagaaataactgtcaaaaatgcaaaggggctaaaacgtacctcttcgatattgaacgaatcaatcgacccttacttatctttcgaatttaacgatatatctattgccaagacaagaaccgtaagagacacattgaaccccgtttgggacgaaactctgtacgtcttgttaaactcattcactgaccccttgaccattagtgtttatgataagcgtgcaaaattgaaggataaagtcctaggcagaattcagtacaacttgaatactttacatgataaaaccacgcaaagaaacttgaaagctcagtttttaagaaactctaaaccggttggggaattgacttttgatttaagattcttcccaactttagaagagaaaaagttgccggatggatctgtggaggaactaccggatttgaatactggtattgctaaagttgtggtcgaagaaggttctcgttttgctgaagaagaacagaaggttactgcctacgttgaagtttacttgaatgctaaactagtgttaaccactggtaaggcaactgacactggtacattgaagtggaattcagactacgaagcagttattgcagatcgtcgtaaaacaagatataagtttgtcgtcaaggatggcaaaggagaagaaattggatctaccattcaaaccctgaatgatttaatagatagaagccaagtgaacaaaaatttgattccattaaaaaaccaaaagggtgacataaagattaccacatattggagacctgttagactggagattggttccaactctgtagcttacacgccaccaattggcgccatcagagtattcattgagaaggcaaacgatttgagaaatttggaaaagtttggtaccattgatccatactgtaaagttttggttaatggtttatcgaagggtagaactgatttcaagagccaaactttgaatcctgtctggaatcaagttatttatgttgcagttacttcaccaaaccaaagaattacgcttcaatgtatggatgtcgaaacagtcaacaaagatcgttcacttggtgaattcaatgttaacgttcaagatttatttaagaaggatgagaatgataaatatgaggaaactattgatgaaaaagctaaagttggtcgtctagtgatgcctaagaaaaaacctaagggcactataacatactatacctccttttatcccgctttacctgttttaacgttagaggaaatccaagatttagataaagttaacaaaaagaaaaaagcattggaactgcgtaagtcagcaattgatgagaaaaaaatttctaaagaagacaaagccaaatttgatcaagaatggaatgaggtcaaagaattggaagacatgtatagtaatagacagaaattggatttgccagaattattgcaatacaaccaaggtgttcttgctgtcactgtccttaatggggaattgcccgactctgggctatacgtccaagcgtttttcgatgataatggtcatccaagatttgttagtccacgtattccatcgagaattgtcaagaatggttggtcaggtgacgtgatcataaaagaattggacaaatccattaccacttttagagttgctaaaaataaaaactacaacagagtggagaaatgcgtttgtgaagtagaactacccacacaagagttagttaagaactgttattataagccatcaatattacatctttcaggtgaaggcagtgctaaactaatgctccaaatatcatggttccctattgatacaaaacagttgccggcaaatgacctgatcacaaattctggtgatttgacgattatgtcgaggagtgcagaaaacttgatagcatccgatttaaatggttattcggacccatacttaaagtattacatcaataatgaggaagactgcgcttacaagacgaaggttgttaagaaaactttgaatcctaaatggaatgatgaaggaactattcaaatcaataaccgtttaaatgatgttctaagaatcaaagtcatggactgggattccacatctgcggatgataccattggtacagcagaaatcccattgaataaggtcaaagtcgagggtactaccgaattagacgtaccggtagaaggattagagaacgctggtcaagacggtggtatgttgcatttagctttcagctttaagccaagatacaccattagcgtgagtaaaagagaaaagaaggtcggagatatagcatccaagggactaggtactggtttgaaagctggtacgaccgtgataggcggcggtgttggtgccattggcaagatcaagaaaggggtattcggtggactaggcagcctcacaaaccataagaagaatcatgagatgggcgaagaagaaactaagttttag

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