LOCUS       NM_001183057            1668 bp    mRNA    linear   PLN 17-DEC-2024
DEFINITION  Saccharomyces cerevisiae S288C
            dolichyl-P-Man:Man(6)GlcNAc(2)-PP-dolichol
            alpha-1,2-mannosyltransferase (ALG9), partial mRNA.
ACCESSION   NM_001183057
VERSION     NM_001183057.1
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Philippsen,P., Kleine,K., Pohlmann,R., Dusterhoft,A., Hamberg,K.,
            Hegemann,J.H., Obermaier,B., Urrestarazu,L.A., Aert,R.,
            Albermann,K., Altmann,R., Andre,B., Baladron,V., Ballesta,J.P.,
            Becam,A.M., Beinhauer,J., Boskovic,J., Buitrago,M.J., Bussereau,F.,
            Coster,F., Crouzet,M., D'Angelo,M., Dal Pero,F., De Antoni,A.,
            Hani,J. et al.
  TITLE     The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV
            and its evolutionary implications
  JOURNAL   Nature 387 (6632 SUPPL), 93-98 (1997)
   PUBMED   9169873
REFERENCE   3  (bases 1 to 1668)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   4  (bases 1 to 1668)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 1668)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 1668)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   7  (bases 1 to 1668)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2010) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   8  (bases 1 to 1668)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001146).
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="XIV"
     gene            <1..>1668
                     /gene="ALG9"
                     /locus_tag="YNL219C"
                     /db_xref="GeneID:855502"
     CDS             1..1668
                     /gene="ALG9"
                     /locus_tag="YNL219C"
                     /EC_number="2.4.1.259"
                     /EC_number="2.4.1.261"
                     /experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
                     reticulum [PMID:26928762]"
                     /experiment="EXISTENCE:direct assay:GO:0006487 protein
                     N-linked glycosylation [PMID:15987956]"
                     /experiment="EXISTENCE:direct assay:GO:0052918
                     dol-P-Man:Man(8)GlcNAc(2)-PP-Dol
                     alpha-1,2-mannosyltransferase activity [PMID:15987956]"
                     /experiment="EXISTENCE:direct assay:GO:0052926
                     dol-P-Man:Man(6)GlcNAc(2)-PP-Dol
                     alpha-1,2-mannosyltransferase activity [PMID:15987956]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0000030
                     mannosyltransferase activity [PMID:8692962]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0005783
                     endoplasmic reticulum [PMID:8692962]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006486 protein
                     glycosylation [PMID:8692962]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006488
                     dolichol-linked oligosaccharide biosynthetic process
                     [PMID:15987956]"
                     /note="Mannosyltransferase, involved in N-linked
                     glycosylation; catalyzes the transfer of both the seventh
                     mannose residue on B-arm and ninth mannose residue on the
                     C-arm from Dol-P-Man to lipid-linked oligosaccharides;
                     human homolog ALG9 can complement yeast null mutant;
                     mutation of human homolog causes type 1 congenital
                     disorders of glycosylation"
                     /codon_start=1
                     /product="dolichyl-P-Man:Man(6)GlcNAc(2)-PP-dolichol
                     alpha-1,2-mannosyltransferase"
                     /protein_id="NP_014180.1"
                     /db_xref="GeneID:855502"
                     /db_xref="SGD:S000005163"
                     /translation="

MNCKAVTISLLLLLFLTRVYIQPTFSLISDCDETFNYWEPLNLLVRGFGKQTWEYSPEYSIRSWAFLLPFYCILYPVNKFTDLESHWNFFITRACLGFFSFIMEFKLHREIAGSLALQIANIWIIFQLFNPGWFHASVELLPSAVAMLLYVGATRHSLRYLSTGSTSNFTKSLAYNFLASILGWPFVLILSLPLCLHYLFNHRIISTIRTAFDCCLIFSLTAFAVIVTDSIFYGKLAPVSWNILFYNVINASEESGPNIFGVEPWYYYPLNLLLNFPLPVLVLAILGIFHLRLWPLWASLFTWIAVFTQQPHKEERFLYPIYGLITLSASIAFYKVLNLFNRKPILKKGIKLSVLLIVAGQAMSRIVALVNNYTAPIAVYEQFSSLNQGGVKAPVVNVCTGREWYHFPSSFLLPDNHRLKFVKSGFDGLLPGDFPESGSIFKKIRTLPKGMNNKNIYDTGKEWPITRCDYFIDIVAPINLTKDVFNPLHLMDNWNKLACAAFIDGENSKILGRAFYVPEPINRIMQIVLPKQWNQVYGVRYIDYCLFEKPTETTN"

     misc_feature    58..1242
                     /gene="ALG9"
                     /locus_tag="YNL219C"
                     /note="Alg9-like mannosyltransferase family; Region:
                     Glyco_transf_22; pfam03901"
                     /db_xref="CDD:281842"
ORIGIN      

atgaattgcaaggcggtaaccattagtttattactgttgttatttttaacaagagtatatattcagccgacattctcgttaatttcagattgcgatgaaacttttaattattgggaaccattaaatttattggtacgtggatttggtaaacaaacctgggaatattcacccgagtattctattagatcatgggctttcttattacctttttactgtattctttatccagtaaacaaatttactgacctagaaagtcattggaactttttcatcacaagagcatgcttaggcttttttagttttatcatggaatttaaactacatcgtgaaattgcaggcagcttggcattgcaaatcgcaaatatttggattattttccaattgtttaatccgggctggttccatgcatctgtggaattattgccttctgccgttgccatgttgttgtatgtaggtgccaccagacactctctacgctatctgtccactgggtctacttctaactttacgaaaagtttagcgtacaatttcctggctagtatactaggctggccatttgttttaattttaagcttgccattatgtttacattaccttttcaaccatagaattatttctaccatcagaaccgcattcgactgctgtttgatattttcattgactgcatttgctgtgattgtcactgacagtatattttacgggaagcttgctcctgtatcatggaacatcttattttacaatgtcattaatgcaagtgaggaatctggcccaaatattttcggggttgagccatggtactactatccactaaatttgttactgaatttcccactgcctgtgctagttttagctattttgggaattttccatttgagattatggccattatgggcatcattattcacatggattgccgttttcactcaacaacctcacaaagaggaaagatttctctatccaatttacgggttaataactttgagtgcaagtatcgccttttacaaagtgttgaatctattcaatagaaagccgattcttaaaaaaggtataaagttgtcagttttattaattgttgcaggccaggcaatgtcacggatagtggctttggtgaacaattacacagctcctatagccgtctacgagcaattttcttcactaaatcaaggtggtgtgaaggcaccggtagtgaatgtatgtacgggacgtgaatggtatcacttcccaagttctttcctgctgccagataatcataggctaaaatttgttaaatctggatttgatggtcttcttccaggtgattttccagagagtggttctattttcaaaaagattagaactttacctaagggaatgaataacaagaatatatatgataccggtaaagagtggccgatcactagatgtgattattttattgacatcgtcgccccaataaatttaacaaaagacgttttcaaccctctacatctgatggataactggaataagctggcatgtgctgcattcatcgacggtgaaaattctaagattttgggtagagcattttacgtaccggagccaatcaaccgaatcatgcaaatagttttaccaaaacaatggaatcaagtgtacggtgttcgttacattgattactgtttgtttgaaaaaccaactgagactactaattga

//