LOCUS       NM_001182925            3537 bp    mRNA    linear   PLN 17-DEC-2024
DEFINITION  Saccharomyces cerevisiae S288C tricalbin (TCB2), partial mRNA.
ACCESSION   NM_001182925
VERSION     NM_001182925.1
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Philippsen,P., Kleine,K., Pohlmann,R., Dusterhoft,A., Hamberg,K.,
            Hegemann,J.H., Obermaier,B., Urrestarazu,L.A., Aert,R.,
            Albermann,K., Altmann,R., Andre,B., Baladron,V., Ballesta,J.P.,
            Becam,A.M., Beinhauer,J., Boskovic,J., Buitrago,M.J., Bussereau,F.,
            Coster,F., Crouzet,M., D'Angelo,M., Dal Pero,F., De Antoni,A.,
            Hani,J. et al.
  TITLE     The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV
            and its evolutionary implications
  JOURNAL   Nature 387 (6632 SUPPL), 93-98 (1997)
   PUBMED   9169873
REFERENCE   3  (bases 1 to 3537)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   4  (bases 1 to 3537)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 3537)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 3537)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   7  (bases 1 to 3537)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2010) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   8  (bases 1 to 3537)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001146).
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="XIV"
     gene            <1..>3537
                     /gene="TCB2"
                     /locus_tag="YNL087W"
                     /db_xref="GeneID:855637"
     CDS             1..3537
                     /gene="TCB2"
                     /locus_tag="YNL087W"
                     /experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
                     reticulum [PMID:26928762]"
                     /experiment="EXISTENCE:direct assay:GO:0005933 cellular
                     bud [PMID:13679573]"
                     /experiment="EXISTENCE:direct assay:GO:0008289 lipid
                     binding [PMID:22250200]"
                     /experiment="EXISTENCE:direct assay:GO:0032541 cortical
                     endoplasmic reticulum [PMID:22250200]"
                     /experiment="EXISTENCE:direct assay:GO:0071944 cell
                     periphery [PMID:26928762]"
                     /experiment="EXISTENCE:genetic interaction:GO:0055091
                     phospholipid homeostasis [PMID:35440494]"
                     /experiment="EXISTENCE:genetic interaction:GO:0060304
                     regulation of phosphatidylinositol dephosphorylation
                     [PMID:23237950]"
                     /experiment="EXISTENCE:genetic interaction:GO:0090158
                     endoplasmic reticulum membrane organization
                     [PMID:23237950]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0035621 ER to
                     Golgi ceramide transport [PMID:33205016]"
                     /note="Lipid-binding ER tricalbin involved in ER-plasma
                     membrane tethering; one of at least 7 proteins (Ice2p,
                     Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering
                     and contact with the plasma membrane (PM); regulates PI4P
                     levels by controlling access of Sac1p to its substrate
                     PI4P in the PM; regulates PM phospholipid homeostasis;
                     contains 3 calcium and lipid binding domains; localizes to
                     the cortical ER, the cell periphery and is enriched in bud
                     membranes; mRNA is targeted to the bud tip"
                     /codon_start=1
                     /product="tricalbin"
                     /protein_id="NP_014312.1"
                     /db_xref="GeneID:855637"
                     /db_xref="SGD:S000005031"
                     /translation="

MSPNSSKTRTDQISSMPGINEATKVESKNVVKDAVPIKSEVETNGTSIVREKQDPSYVGWKQVGGWEEKDELTSEDLLVDVNKDTFLGNLLPDKFYGDWYHEVAILIIAGLCSFVLGYFKFSLASVLIVMLTTGMLYRTSSKKYRESLRDLAQKEQTVEKITSDYESVEWLNTFLDKYWPIIEPSVSQQIVDGTNTALSENVAIPKFIKAIWLDQFTLGVKPPRIDAIKTFQNTKSDVVVMDVCLSFTPHDMSDLDAKQCRNYVNSNVVLKAKIFGMDIPVSVADIFFQVFVRFRFQLMTTLPLVETINIQLLEVPEVDFISRLLGNSVFNWEILAIPGLMRLIQKMAFKYLSPVLLPPFSLQLNIPQLLSKTGLPIGVLEIKVKNAHGLRKLVGMIKKTVDPYLTFELSGKIVGKTKVFKNSANPVWNESIYILLQSFTDPLTIAVYDKRETLSDKKMGTVIFNLNKLHANHYHKNEKVHFLRNSKPVGELTFDLRFFPTIEPKKLLNGDEEPLPDMNTGITKITIRELKGLDELSDKKFVFAELYVNAELVMTTKKEKRTAHLKWNSDYYSVVTDRRKTICRFVLKDQSGKVISSSVQPLNHLIDRTEVNKEWIPLRNGKGELKVTTYWRPVDIDLGLKSVGYTTPIGMLRVFINKAENLRNPDSLGKISPYAKVSVNGVARGRTNERIETLNPIWNQSIYVSVTSPLQKVSIDCFGIDTNGDDHNLGSLNIQTQNIYHKDNDDKYTIFIDNAPRTGNLIGKKGVKGTVTYYLSFYPVVPVLSLEEAKEVDEINEKKDKLEKQKSTLDDKNISKEEKERIKKEEFRLTEKYDMYSYKMKLDLDELLQYNAGVLGVTVLGGELPQPGLYVQTFFDSCGYAAITSAKNAIRTIKTGWSGDFMIKELEWSVTTFRVTKTKDANKAENFICEVNIPTIELVRNCYYKPSVLNLIGKKSAKLLVQVSWFPVTATELPQSDLITNSGDLKITAKSAENLIGVNKNGYSDPYVEFFLNEKSTSPFFKTAVQKKTLNPTWNESKTIEVSNRVNDYLTINVKDYESTNSNRSIGKAVVPLSTIDPESDTTFNIPLVGPKGEDGGVLHLEFEFEPRYTTNVVKREAGLGNFATKGLGTGIKAGSTVFALGTNVVSTGLGTIDKVKAGVFGGKKSTTTGDKKSEEKQ"

     misc_feature    10..3534
                     /gene="TCB2"
                     /locus_tag="YNL087W"
                     /note="Ca2+-dependent lipid-binding protein, contains C2
                     domain [General function prediction only]; Region:
                     COG5038"
                     /db_xref="CDD:227371"
ORIGIN      

atgtctccaaactcgtcgaaaactcgaacggaccagataagctccatgcctggtattaatgaagccacgaaggtggaaagtaagaatgttgtgaaggatgctgttccaataaaatctgaagttgaaaccaatggtacatcaatagtaagagaaaaacaagatccatcgtacgttggttggaaacaagttggtggttgggaggaaaaggacgagttaacttccgaagaccttttagtggacgtaaacaaggacacttttttggggaaccttttaccagataaattctatggtgattggtatcatgaagtcgctattctcatcatcgctggtttatgttcctttgtgctgggctattttaaattctctctcgcgtccgtccttatcgtgatgctaaccacaggaatgttatatagaacttcgtccaaaaaatatagggaatcgttaagggatttagcccagaaagaacaaactgtagagaaaattactagtgattatgaatctgtcgaatggttaaacacctttttagataaatactggcccattatcgaaccttctgtctcccaacaaattgtcgatggtacaaatactgcattatctgaaaatgtggcaattccaaagtttataaaagccatctggctggatcaatttacgttaggtgtgaagcctccaagaattgatgccataaagacattccaaaatacaaaatctgatgtcgtagtcatggacgtatgtctatcattcactccacacgacatgtctgatcttgatgcaaaacaatgcaggaattacgtaaattccaatgtcgtattaaaggcaaagatattcggaatggacatacctgtttcagttgctgatattttttttcaagtttttgttagatttcgtttccaattgatgacaactttgcctcttgttgaaactataaatattcagttattggaagttcctgaagttgattttattagtcgtttattgggtaactccgtctttaattgggaaatcctggctattccaggcttaatgagattgattcaaaaaatggcatttaaatacttgagccctgttttactcccacctttctctttgcaacttaatataccacaacttctttccaagactggcttacctataggtgttttggaaatcaaagtcaaaaatgcccatggattaagaaaacttgtgggcatgatcaagaaaacagttgacccatacttgacatttgagctctctggtaaaatagtcggtaaaactaaggtttttaaaaattctgctaatcctgtttggaatgaatccatttacattctgttgcaatcttttactgatccacttacaatagccgtatatgacaaacgcgaaaccttaagcgacaaaaaaatgggtacagtaattttcaacttgaataagttacatgcaaatcactatcacaaaaatgaaaaggtacatttcctaagaaattctaaacctgtcggtgagttgacatttgatttgcgatttttccccactattgaaccaaaaaaattactgaacggtgatgaggagcctttaccggatatgaatacaggtatcaccaaaattactattcgcgaactcaagggactggatgaactaagtgacaaaaaattcgtctttgcagagttgtatgtcaatgcagaattggtgatgaccacaaagaaagagaaaagaactgcccatttgaaatggaactcagattattattctgtggtaacagaccgtagaaagactatctgtagatttgttttgaaggatcagagtggtaaagtaatttcatcttcggtacaaccattaaatcatttgatagatagaacagaagtcaataaagaatggattcctttaaggaatggaaagggagaattaaaagtcaccacttactggaggccggtagatatcgacctcggattgaaaagtgtaggatatactactcctatcggtatgttgagagtgtttatcaataaggctgaaaacttgagaaatccagatagcttaggaaagataagtccatacgccaaagtatccgtcaatggtgtggcaagaggtaggacaaatgaaagaatagagacattgaatcctatctggaaccaatcaatttatgtgtctgtcacatctcccttacaaaaagtctcaattgactgtttcggtatagatacaaatggcgatgatcataatctcgggagccttaatattcaaactcagaatatatatcataaagacaatgacgataaatataccatttttattgacaacgctccaagaactggcaacttaattggaaagaagggtgtaaaggggaccgttacgtattatttatcattttatccagttgttcctgttttaagcttggaagaggcgaaggaagttgacgagattaatgagaaaaaagataagctagaaaagcagaaaagtacactggatgacaaaaacatctcaaaggaggagaaagaaagaattaaaaaggaagagttcaggctcaccgaaaaatatgacatgtatagttacaaaatgaagcttgacctcgatgaattgctgcaatacaatgctggtgtactaggtgttactgtattaggcggggaactaccccagcctgggctctatgttcaaacattttttgattcctgtggttacgcagcgataactagtgcaaaaaatgctattaggaccattaaaacaggctggtcgggcgatttcatgataaaagagctggaatggtccgtgactacatttagggtaacaaagactaaagacgcgaataaggcggaaaattttatttgcgaagtcaacattccaacaattgaattggtgaggaactgttattacaaaccttccgtattaaatctaatagggaaaaagagtgccaaactattagttcaagtgtcatggttccccgtaactgccacggaattgcctcaatcagacttgataactaactctggtgatttgaaaatcaccgctaaaagtgctgaaaatctaattggtgttaataagaatggttattccgatccatatgtcgaattctttttgaatgaaaagagcacatcacccttctttaaaaccgcagttcaaaaaaagacactaaatcctacctggaacgaaagcaaaactatcgaagtttcaaatcgtgtcaatgactacttgacaattaacgtgaaagactacgaatccactaactccaataggagtattggaaaagccgttgttccattatctactattgatccagagagcgataccacctttaatataccgttagtgggaccaaagggagaagatggcggtgtgctacatcttgaatttgagtttgaaccccgctatactaccaatgtagtcaaacgtgaagccgggcttggaaattttgccacgaaaggattaggtacaggcatcaaagctggttctacagttttcgccttaggaaccaatgttgtaagcaccggattaggaacaattgataaagtaaaggccggagtctttggtggcaagaagtctacaaccactggggacaaaaaatccgaagagaagcaataa

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