LOCUS       NM_001181641             990 bp    mRNA    linear   PLN 17-DEC-2024
DEFINITION  Saccharomyces cerevisiae S288C ribonuclease (NUC1), partial mRNA.
ACCESSION   NM_001181641
VERSION     NM_001181641.1
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 990)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   3  (bases 1 to 990)
  AUTHORS   Galibert,F., Alexandraki,D., Baur,A., Boles,E., Chalwatzis,N.,
            Chuat,J.C., Coster,F., Cziepluch,C., De Haan,M., Domdey,H.,
            Durand,P., Entian,K.D., Gatius,M., Goffeau,A., Grivell,L.A.,
            Hennemann,A., Herbert,C.J., Heumann,K., Hilger,F., Hollenberg,C.P.,
            Huang,M.E., Jacq,C., Jauniaux,J.C., Katsoulou,C.,
            Karpfinger-Hartl,L. et al.
  TITLE     Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
            X
  JOURNAL   EMBO J. 15 (9), 2031-2049 (1996)
   PUBMED   8641269
REFERENCE   4  (bases 1 to 990)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 990)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 990)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   7  (bases 1 to 990)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001142).
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="X"
     gene            <1..>990
                     /gene="NUC1"
                     /locus_tag="YJL208C"
                     /db_xref="GeneID:853222"
     CDS             1..990
                     /gene="NUC1"
                     /locus_tag="YJL208C"
                     /experiment="EXISTENCE:direct assay:GO:0004520 DNA
                     endonuclease activity [PMID:3286639]"
                     /experiment="EXISTENCE:direct assay:GO:0004529 DNA
                     exonuclease activity [PMID:3286639]"
                     /experiment="EXISTENCE:direct assay:GO:0004540 RNA
                     nuclease activity [PMID:3286639]"
                     /experiment="EXISTENCE:direct assay:GO:0005634 nucleus
                     [PMID:17244531]"
                     /experiment="EXISTENCE:direct assay:GO:0005739
                     mitochondrion [PMID:24769239|PMID:16823961]"
                     /experiment="EXISTENCE:direct assay:GO:0005743
                     mitochondrial inner membrane [PMID:3286639|PMID:17244531]"
                     /experiment="EXISTENCE:direct assay:GO:0005829 cytosol
                     [PMID:31266891]"
                     /experiment="EXISTENCE:direct assay:GO:0006308 DNA
                     catabolic process [PMID:3286639]"
                     /experiment="EXISTENCE:direct assay:GO:0006401 RNA
                     catabolic process [PMID:3286639]"
                     /experiment="EXISTENCE:direct assay:GO:0051607 defense
                     response to virus [PMID:31266891]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006309
                     apoptotic DNA fragmentation [PMID:22727375]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006310 DNA
                     recombination [PMID:8087883]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006915
                     apoptotic process [PMID:17244531]"
                     /note="Major mitochondrial nuclease; has RNAse and DNA
                     endo- and exonucleolytic activities; prevents genome
                     instability by degrading extranuclear DNA sequences; roles
                     in mitochondrial recombination, apoptosis and maintenance
                     of polyploidy; involved in fragmentation of genomic DNA
                     during PND (programmed nuclear destruction); role in
                     preventing L-A mycovirus pathogenesis; encodes ortholog of
                     mammalian endoG"
                     /codon_start=1
                     /product="ribonuclease"
                     /protein_id="NP_012327.1"
                     /db_xref="GeneID:853222"
                     /db_xref="SGD:S000003744"
                     /translation="

MCSRILLSGLVGLGAGTGLTYLLLNKHSPTQIIETPYPPTQKPNSNIQSHSFNVDPSGFFKYGFPGPIHDLQNREEFISCYNRQTQNPYWVLEHITPESLAARNADRKNSFFKEDEVIPEKFRGKLRDYFRSGYDRGHQAPAADAKFSQQAMDDTFYLSNMCPQVGEGFNRDYWAHLEYFCRGLTKKYKSVRIVTGPLYLPKKDPIDNKFRVNYEVIGNPPSIAVPTHFFKLIVAEAPTANPAREDIAVAAFVLPNEPISNETKLTDFEVPIDALERSTGLELLQKVPPSKKKALCKEVNCQIVVRDFSNAAIKQSKDVKLLPPPKKRN"

     misc_feature    220..870
                     /gene="NUC1"
                     /locus_tag="YJL208C"
                     /note="DNA/RNA non-specific endonuclease; Region: NUC;
                     smart00477"
                     /db_xref="CDD:214683"
     misc_feature    order(403..408,412..414,508..510,532..534,544..546)
                     /gene="NUC1"
                     /locus_tag="YJL208C"
                     /note="active site"
                     /db_xref="CDD:238043"
     misc_feature    order(403..408,544..546)
                     /gene="NUC1"
                     /locus_tag="YJL208C"
                     /note="substrate binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:238043"
     misc_feature    508..510
                     /gene="NUC1"
                     /locus_tag="YJL208C"
                     /note="Mg2+ binding site [ion binding]; other site"
                     /db_xref="CDD:238043"
ORIGIN      

atgtgcagtaggatactcttgtccggtttagtcggactgggtgctggtactggcttaacctatcttcttttgaacaaacattctccaacgcaaatcattgagacaccttatccacctacccagaaacctaatagtaatattcaatctcactctttcaacgtcgatccttccgggttcttcaagtatggttttcctggtccgattcatgatctgcagaaccgcgaagagtttatctcatgttacaacagacaaactcaaaatccttattgggtcctcgaacatataacgccagaatcattggctgcaaggaatgctgacagaaaaaattcctttttcaaggaagatgaagtaattccagaaaagtttagaggtaaactaagagactattttaggtcgggctatgatcgaggccatcaagccccagctgcagacgcaaaattttctcaacaggccatggatgatacattctacttatccaatatgtgtcctcaagtaggagaaggttttaatagagactattgggcgcatttggagtacttttgtaggggattgactaagaaatataagagtgtaagaatcgtgactggtccattgtatctacccaaaaaggatcccatagataataaatttagggttaattatgaagttattggcaatccacccagtattgctgttccaacgcacttttttaaattgattgttgcagaagcaccaacagccaatcctgctagagaggatattgctgtcgcggcatttgtattgccaaacgaaccgatatcaaatgagacgaaattgactgactttgaagttcctatagatgctttagagagaagtactgggctagaacttctgcaaaaagtaccaccttcaaagaagaaggcattatgcaaagaggtaaattgtcaaattgtagtgagagatttctctaacgcggcgatcaaacaatcgaaagatgtgaaattgttacctcctccaaaaaaaaggaattga

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