LOCUS       NM_001181227            4893 bp    mRNA    linear   PLN 15-SEP-2023
DEFINITION  Saccharomyces cerevisiae S288C separase (ESP1), partial mRNA.
ACCESSION   NM_001181227
VERSION     NM_001181227.3
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Tettelin,H., Agostoni Carbone,M.L., Albermann,K., Albers,M.,
            Arroyo,J., Backes,U., Barreiros,T., Bertani,I., Bjourson,A.J.,
            Bruckner,M., Bruschi,C.V., Carignani,G., Castagnoli,L., Cerdan,E.,
            Clemente,M.L., Coblenz,A., Coglievina,M., Coissac,E., Defoor,E.,
            Del Bino,S., Delius,H., Delneri,D., de Wergifosse,P., Dujon,B.,
            Kleine,K. et al.
  TITLE     The nucleotide sequence of Saccharomyces cerevisiae chromosome VII
  JOURNAL   Nature 387 (6632 SUPPL), 81-84 (1997)
   PUBMED   9169869
REFERENCE   3  (bases 1 to 4893)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   4  (bases 1 to 4893)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2023) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 4893)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2015) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 4893)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   7  (bases 1 to 4893)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   8  (bases 1 to 4893)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001139).
            
            On Jul 30, 2012 this sequence version replaced NM_001181227.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="VII"
     gene            <1..>4893
                     /gene="ESP1"
                     /locus_tag="YGR098C"
                     /db_xref="GeneID:852990"
     CDS             1..4893
                     /gene="ESP1"
                     /locus_tag="YGR098C"
                     /EC_number="3.4.22.49"
                     /experiment="EXISTENCE:direct assay:GO:0004197
                     cysteine-type endopeptidase activity
                     [PMID:11081626|PMID:11081625]"
                     /experiment="EXISTENCE:direct assay:GO:0005634 nucleus
                     [PMID:11149918|PMID:22842922]"
                     /experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
                     [PMID:11149918|PMID:22842922]"
                     /experiment="EXISTENCE:direct assay:GO:0005739
                     mitochondrion [PMID:14576278|PMID:16823961]"
                     /experiment="EXISTENCE:direct assay:GO:0005819 spindle
                     [PMID:11149918]"
                     /experiment="EXISTENCE:genetic interaction:GO:0031536
                     positive regulation of exit from mitosis [PMID:14551257]"
                     /experiment="EXISTENCE:genetic interaction:GO:0032888
                     regulation of mitotic spindle elongation [PMID:18430955]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0000070 mitotic
                     sister chromatid segregation [PMID:9635435]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0004197
                     cysteine-type endopeptidase activity [PMID:11081626]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006915
                     apoptotic process [PMID:18321989]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0031536
                     positive regulation of exit from mitosis
                     [PMID:27418100|PMID:14551257]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0032888
                     regulation of mitotic spindle elongation [PMID:18430955]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0035308
                     negative regulation of protein dephosphorylation
                     [PMID:16713564]"
                     /experiment="EXISTENCE:mutant phenotype:GO:1904750
                     negative regulation of protein localization to nucleolus
                     [PMID:14551257]"
                     /experiment="EXISTENCE:physical interaction:GO:0035308
                     negative regulation of protein dephosphorylation
                     [PMID:16713564]"
                     /note="Separase/separin, a caspase-like cysteine protease;
                     cleaves Mcd1p/Scc1p, a mitotic cohesin complex subunit,
                     resulting in the dissociation of cohesin from chromatin
                     and sister chromatid separation; cleaves meiotic-cohesin
                     subunit Rec8p along chromosome arms in meiosis I and at
                     centromeric sites during meiosis II; inhibits PP2A-Cdc55p
                     to promote mitotic exit; inhibited by Pds1p (securin);
                     relative distribution to the nucleus increases upon DNA
                     replication stress"
                     /codon_start=1
                     /product="separase"
                     /protein_id="NP_011612.3"
                     /db_xref="GeneID:852990"
                     /db_xref="SGD:S000003330"
                     /translation="

MMVKQEEPLNEISPNTPMTSKSYLLNDTLSKVHHSGQTRPLTSVLSGDASSNSIGILAMHNNIIRDFTKIASNNIDLAIEDITTVDHSLNSIYSLLKSHHMWGHINSTVKQHLMIIVKLINNNALGLASSEIIFLFNETNLFQAHSLKNILLADFSTWNDYYLSNLKILALQIILKRKLVDEYLPHILELFSHDKRYLLKDPNLKAHALTKIVLSFFSVTTSCKVLFGLKFLQYIKQFKLPFKKFISNITVECFSKNLLHKNYLEMGPNKIYLNSFYLSYSMLYDGLDKIMLLDILSYEETTEVQRAIKSKKEFNEYCNMSENRLLWSCISVDDLNVILENATNFLQNKGKHISATLKCLVCLWSTIRLEGLPKNKDILRQFDCTVIYINSNIKSINDESAAALLSELLGVLSEICIDYKEPKRLSNIISVLFNASVLFKSHSFLLKTANLEISNVLISNDSKTSHRTILKFEKFISSAQSAQKKIEIFSCLFNVYCMLRNDTLSFVFDFCQNAFIHCFTRLKITKFIEFSNSSEIMLSVLYGNSSIENIPSENWSQLSRMIFCSLRGIFDLDPLELNNTFDKLHLLNKYELLIRIVYLLNLDMSKHLTTNLSKITKLYINKWLQKSDEKAERISSFEMDFVKMLLCYLNFNNFDKLSIELSLCIKSKEKYYSSIVPYADNYLLEAYLSLYMIDDALMMKNQLQKTMNLSTAKIEQALLHASSLINVHLWDSDLTAFQIYFGKTLPAMKPELFDINNDHNLPMSLYIKVILLNIKIFNESAKLNIKAGNVISAVIDCRKAQNLALSLLKKKNKLSQGSRLALLKSLSFSFFQLIKIHIRIGSARDCEFYSKELSRIISDLEEPIIVYRCLHFLHRYYMITEQTCLQNITLGKANKAFDYLDAEADITSLTMFLYDNKEFVKLEQSLVLYFGDQLEKTFLPNLWKLHLGKDIDDSICLSEYMPKNVINRVHNMWQKVMSQLEEDPFFKGMFESTLGIPSSLPVIPSTMPNNILKTPSKHSTGLKLCDSPRSSSMTPRGKNIRQKFDRIAAISKLKQMKELLESLKLDTLDNHELSKISSLSSLTLTILSNITSIHNAESSLITNFSLTDLPRHMPLLFDKVLNNIDNKNYREFRVSSLIAPNNISTITESIRVSAAQKDLMESNLNINVITIDFCPITGNLLLSKLEPRRKRRTHLRLPLIRSNSRDLDEVHLSFPEATKKLLSIINESNQTTSVEVTNKIKTREERKSWWTTRYDLDKRMQQLLNNIENSWFNGVQGFFSPEVVDNSLFEKFKDKFYEILHQNLPSRKLYGNPAMFIKVEDWVIELFLKLNPQEIDFLSKMEDLIYFVLDILLFHGEENAYDEIDFSMLHVQLEEQIKKYRATMTTNSIFHTFLVVSSSCHLFPWECLSFLKDLSITRVPSYVCLNKLLSRFHYQLPLQVTIEDNISMILNPNGDLSRTESKFKGMFQKIIDAKPSSQLVMNEKPEEETLLKMLQNSNLFVYIGHGGGEQYVRSKEIKKCTKIAPSFLLGCSSAAMKYYGKLEPTGTIYTYLLGGCPMVLGNLWDVTDKDIDKFSEELFEKMGFRCNTDDLNGNSLSVSYAVSKSRGVCHLRYLNGAAPVIYGLPIKFVS"

     misc_feature    1..4890
                     /gene="ESP1"
                     /locus_tag="YGR098C"
                     /note="Separase, a protease involved in sister chromatid
                     separation [Cell division and chromosome partitioning /
                     Posttranslational modification, protein turnover,
                     chaperones]; Region: ESP1; COG5155"
                     /db_xref="CDD:227484"
ORIGIN      

atgatggtaaaacaggaggaaccgctgaatgagattagtccgaatactccgatgactagcaagtcctaccttttaaacgacacgctttcgaaagtacatcatagtggccaaactagaccactgacctcggtgttgtctggagacgcctcatccaattctattggcatactagcaatgcataataacataatacgtgactttacaaaaattgcaagcaataacatagacttagccattgaggatattaccactgttgaccattcattaaattcaatatatagcctattgaagagccatcacatgtggggccatataaactccactgttaaacaacatttgatgataattgttaaactaataaataataacgcattaggattggcctcctctgaaattatttttcttttcaatgaaacgaatttatttcaagctcatagtctgaaaaacattcttctagcagacttcagtacgtggaacgactactatctttcaaatctgaaaatattagcgctgcagattatactgaaaagaaagctagtagacgaatacttaccgcatatattagaactattttcccatgataaaagatatcttttgaaagaccctaacctcaaagctcacgctctcacaaaaatcgtgctgagctttttttctgtcacgacgagttgcaaagttctattcgggctaaaatttctacaatatatcaaacagttcaaattaccttttaaaaagttcattagcaacattaccgtcgagtgttttagcaaaaaccttcttcataaaaattatttggagatgggacctaataaaatatatttaaattcattttacttatcctattccatgctgtacgatggattggataaaataatgcttctagacattttaagttacgaagaaactactgaggtacaaagagcgataaaaagtaaaaaagaattcaatgaatactgcaacatgagtgaaaaccgtcttctctggtcctgtatatctgtcgatgatttgaacgtaatactcgaaaatgcaacgaattttctacaaaataaagggaaacatatatccgcaacattaaaatgtttagtctgtctttggtctactattcgcctagagggattacctaaaaataaggatatattgaggcagtttgattgtactgttatttatatcaactcaaatattaaatccattaatgacgaatctgcggcggcattattatccgaacttctgggagttttatcagaaatttgtatcgattataaagagcctaaacgactgtcaaatattatcagtgtccttttcaatgcttctgttttatttaagagtcattcgtttttgttaaagactgctaatttggaaatctcaaatgttttaatcagtaatgattcaaaaacatctcatcgtactatactgaaatttgaaaaatttatatctagtgctcagagtgcccagaaaaaaattgaaatcttttcctgtttgttcaatgtatattgcatgctacgaaatgatacgctatcatttgtttttgacttttgccaaaatgcattcattcattgctttacacgcctaaaaatcacaaaattcatcgagttttcaaacagttcagaaattatgctttcagtactgtatggaaattcatctatcgaaaacattccttccgaaaattggtcacaactaagcagaatgatattttgctcactgaggggtatctttgatttggatcctttagagctaaataatacgtttgataaattacacttgctcaacaaatatgagctattaatacgaatagtgtacctattgaacctggacatgagtaaacacctgacaacgaatctatcgaaaataactaagctgtacataaacaaatggctacaaaaatcagatgaaaaggccgagagaatatcgtcatttgaaatggacttcgttaaaatgctattgtgctacttgaactttaataattttgataagttatccattgaactttcactctgtattaaatcaaaagaaaagtattactccagtattgtgccttatgccgacaattatttgctagaggcttatctgagcttatatatgattgatgatgccctcatgatgaagaatcaactacaaaaaacaatgaatttatcaacggcgaaaatagagcaggcgttattacatgcaagttctttaataaatgtccacttgtgggactctgacttgacagcgtttcaaatatattttggaaaaacactgcctgctatgaagcctgaattatttgatatcaataacgaccacaacctacccatgtcactttacatcaaagtcatacttttgaacataaaaatttttaatgaaagcgcgaaattaaatattaaagctggtaatgttatatccgcagtaattgattgtagaaaagcacaaaatttagcattatcgttgctcaaaaagaagaacaaattgtcacaaggatccaggctagcattactaaaaagtctatcgtttagttttttccaactcattaaaatacatatacgcattggttctgccagagactgtgagttctacagtaaagaactgtctagaattatttctgacttagaggaacctattatcgtctacagatgtctgcattttcttcacagatactatatgattacagagcaaacctgtcttcaaaatataactttgggaaaagccaataaggcatttgattatcttgatgctgaggcagacattacaagtttgacaatgtttctgtatgataataaggaatttgtaaagttagagcagtcgttggtcctatatttcggtgaccagctagaaaaaacattcctacctaatttgtggaagcttcacttgggtaaggatattgacgacagcatttgtctgtccgaatatatgcccaaaaatgtaatcaatagagtacacaatatgtggcaaaaagtaatgagccaattggaggaagatcctttcttcaaaggtatgtttgaatctacccttgggattccctcttctctgcctgttattcctagcacgatgcccaacaacattttaaaaactccttccaagcatagtacaggattgaagctttgcgattcaccaagatcgtcgagcatgacgcctagaggtaagaatataagacagaaatttgatagaattgctgctattagtaaattaaaacagatgaaagaacttttggagagtttgaaactagacacattagataatcacgaactgtccaaaatttcctccctttcttcattaactctgactatcctttcaaacataactagtatccataatgctgagagtagtttgataactaatttttcgctaactgacttgccaaggcatatgcctttgttattcgataaggtattgaataatattgataataaaaattaccgagaatttcgcgtttcatcactaatagctccgaacaatatatccacaattacagagagtattagagtatctgccgcccagaaggacttaatggagagcaaccttaatattaatgttataactattgacttctgtcccataactggaaatttacttctatccaaattagaaccgcgacgtaagagaaggacgcatttaaggctaccgctaatacgatctaattcaagagacctcgacgaagttcatctctcatttccggaggctacaaaaaaactactttctattatcaatgaaagcaaccaaacgacgtcagtggaagttaccaacaagattaaaacaagagaagaaagaaaatcttggtggaccacaagatatgatttggataaaagaatgcaacaattgttgaataacattgaaaatagttggttcaatggcgttcaagggttttttagccctgaagtagtcgacaactcgttatttgaaaaatttaaagataaattttacgaaattctgcatcaaaatcttccttccagaaaactctatggcaatccggcaatgtttataaaagttgaagattgggtaatcgaattgttcttgaaacttaatccgcaagaaatcgattttctttcgaagatggaagacctgatatactttgttcttgatattcttctcttccatggtgaagaaaatgcttacgacgaaattgatttcagcatgcttcacgtgcagttagaagagcaaataaagaaatatagggcaactatgacaactaatagtatatttcatacatttttagtcgttagcagttcatgccatttattcccttgggagtgtctttcatttttgaaagatttgtctatcacacgtgttccgtcctatgtttgtctgaataaacttttatcgcgtttccattaccaactaccccttcaagtaactattgaggacaacattagtatgatattaaatccgaacggagatttgtccaggactgaatctaaattcaagggtatgttccaaaaaataatcgatgcaaaaccttcctctcaattagtgatgaacgagaagccggaagaggaaacacttttgaaaatgctgcaaaattctaacttattcgtttatatagggcatggcggaggtgaacaatatgtaaggagtaaggagattaagaaatgcaccaaaattgcccccagctttttactgggctgctcttcagcagcgatgaaatattacggcaagttagaacctactggaacaatctatacttatttgctgggaggatgccctatggtactaggtaatctgtgggatgtaactgacaaagatattgataaatttagcgaggaactttttgaaaagatgggatttcgctgtaatactgacgatttgaatggtaatagcctatctgtttcatatgccgtctctaaatctagaggtgtatgccatctacgttacttgaacggcgcagctcctgttatttatgggttaccgatcaagttcgtatcataa

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