LOCUS       NM_001180062            1701 bp    mRNA    linear   PLN 15-SEP-2023
DEFINITION  Saccharomyces cerevisiae S288C kleisin alpha (MCD1), partial mRNA.
ACCESSION   NM_001180062
VERSION     NM_001180062.1
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Jacq,C., Alt-Morbe,J., Andre,B., Arnold,W., Bahr,A., Ballesta,J.P.,
            Bargues,M., Baron,L., Becker,A., Biteau,N., Blocker,H., Blugeon,C.,
            Boskovic,J., Brandt,P., Bruckner,M., Buitrago,M.J., Coster,F.,
            Delaveau,T., del Rey,F., Dujon,B., Eide,L.G.,
            Garcia-Cantalejo,J.M., Goffeau,A., Gomez-Peris,A., Zaccaria,P. et
            al.
  TITLE     The nucleotide sequence of Saccharomyces cerevisiae chromosome IV
  JOURNAL   Nature 387 (6632 SUPPL), 75-78 (1997)
   PUBMED   9169867
REFERENCE   3  (bases 1 to 1701)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   4  (bases 1 to 1701)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2023) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 1701)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2015) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 1701)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2013) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   7  (bases 1 to 1701)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   8  (bases 1 to 1701)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   9  (bases 1 to 1701)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001136).
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="IV"
     gene            <1..>1701
                     /gene="MCD1"
                     /locus_tag="YDL003W"
                     /gene_synonym="PDS3; RHC21; SCC1"
                     /db_xref="GeneID:851561"
     CDS             1..1701
                     /gene="MCD1"
                     /locus_tag="YDL003W"
                     /gene_synonym="PDS3; RHC21; SCC1"
                     /experiment="EXISTENCE:direct assay:GO:0000794 condensed
                     nuclear chromosome [PMID:18614046]"
                     /experiment="EXISTENCE:direct assay:GO:0003682 chromatin
                     binding [PMID:18614046|PMID:9990856]"
                     /experiment="EXISTENCE:direct assay:GO:0005634 nucleus
                     [PMID:15282802]"
                     /experiment="EXISTENCE:direct assay:GO:0005739
                     mitochondrion [PMID:18321989]"
                     /experiment="EXISTENCE:direct assay:GO:0030892 mitotic
                     cohesin complex [PMID:9990856]"
                     /experiment="EXISTENCE:genetic interaction:GO:0006974 DNA
                     damage response [PMID:27325700]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006302
                     double-strand break repair [PMID:11448778|PMID:23178808]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006915
                     apoptotic process [PMID:18321989]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
                     damage response [PMID:18614046]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0007076 mitotic
                     chromosome condensation [PMID:9335334]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0034087
                     establishment of mitotic sister chromatid cohesion
                     [PMID:16962805]"
                     /experiment="EXISTENCE:mutant phenotype:GO:1990414
                     replication-born double-strand break repair via sister
                     chromatid exchange [PMID:16888651]"
                     /experiment="EXISTENCE:physical interaction:GO:0019901
                     protein kinase binding [PMID:27325700]"
                     /note="Essential alpha-kleisin subunit of the cohesin
                     complex; required for sister chromatid cohesion in
                     mitosis; subject to proteolytic cleavage by separase
                     Esp1p, resulting in dissociation of cohesin from chromatin
                     and the separation of sister chromatids at the mitotic
                     metaphase-to-anaphase transition; apoptosis induces
                     cleavage and translocation of a C-terminal fragment to
                     mitochondria; expression peaks in S phase"
                     /codon_start=1
                     /product="kleisin alpha"
                     /protein_id="NP_010281.1"
                     /db_xref="GeneID:851561"
                     /db_xref="SGD:S000002161"
                     /translation="

MVTENPQRLTVLRLATNKGPLAQIWLASNMSNIPRGSVIQTHIAESAKEIAKASGCDDESGDNEYITLRTSGELLQGIVRVYSKQATFLLTDIKDTLTKISMLFKTSQKMTSTVNRLNTVTRVHQLMLEDAVTEREVLVTPGLEFLDDTTIPVGLMAQENSMERKVQGAAPWDTSLEVGRRFSPDEDFEHNNLSSMNLDFDIEEGPITSKSWEEGTRQSSRNFDTHENYIQDDDFPLDDAGTIGWDLGITEKNDQNNDDDDNSVEQGRRLGESIMSEEPTDFGFDLDIEKEAPAGNIDTITDAMTESQPKQTGTRRNSKLLNTKSIQIDEETENSESIASSNTYKEERSNNLLTPQPTNFTTKRLWSEITESMSYLPDPILKNFLSYESLKKRKIHNGREGSIEEPELNVSLNLTDDVISNAGTNDNSFNELTDNMSDFVPIDAGLNEAPFPEENIIDAKTRNEQTTIQTEKVRPTPGEVASKAIVQMAKILRKELSEEKEVIFTDVLKSQANTEPENITKREASRGFFDILSLATEGCIGLSQTEAFGNIKIDAKPALFERFINA"

     misc_feature    49..324
                     /gene="MCD1"
                     /locus_tag="YDL003W"
                     /gene_synonym="PDS3; RHC21; SCC1"
                     /note="N-terminus of Rad21 / Rec8 like protein; Region:
                     Rad21_Rec8_N; pfam04825"
                     /db_xref="CDD:428144"
     misc_feature    943..1182
                     /gene="MCD1"
                     /locus_tag="YDL003W"
                     /gene_synonym="PDS3; RHC21; SCC1"
                     /note="Middle HEAT-repeat binding domain found in
                     Saccharomyces cerevisiae sister chromatid cohesion protein
                     1 (ScScc1p) and similar proteins; Region:
                     Rad21_Rec8_M_ScScc1p-like; cd21791"
                     /db_xref="CDD:439293"
     misc_feature    order(952..960,967..990,997..999,1006..1011,1024..1047,
                     1075..1077,1081..1086,1093..1110,1123..1128,1132..1155)
                     /gene="MCD1"
                     /locus_tag="YDL003W"
                     /gene_synonym="PDS3; RHC21; SCC1"
                     /note="putative HEAT-repeat binding site [polypeptide
                     binding]; other site"
                     /db_xref="CDD:439293"
     misc_feature    1513..1677
                     /gene="MCD1"
                     /locus_tag="YDL003W"
                     /gene_synonym="PDS3; RHC21; SCC1"
                     /note="Conserved region of Rad21 / Rec8 like protein;
                     Region: Rad21_Rec8; pfam04824"
                     /db_xref="CDD:335912"
ORIGIN      

atggttacagaaaatcctcaacgtcttactgttttaagacttgccaccaataagggtccattagcacaaatatggcttgcttcaaatatgtcgaatataccaagaggatcggttatccaaacacatattgctgaatcagcaaaggaaattgcaaaagcttctggctgtgatgacgaatcaggggacaatgaatacataactttacgaacatctggcgaattacttcaaggcattgttcgtgtatattccaaacaggccacttttctccttacagatataaaggacacattaacgaagataagtatgttatttaaaacaagccagaagatgacttctacagtaaacagactgaacactgtaacaagggttcatcaattgatgttagaagatgccgttacagaaagagaagttttggtcaccccagggttagagttcttagatgatactactataccagtgggcttaatggcgcaggagaattcgatggaaagaaaagtccaaggtgctgctccctgggatacttcacttgaggtcggtagacgattcagtccagatgaagatttcgaacataataatttgtctagtatgaaccttgattttgacatcgaggaaggtccgataacttcaaaatcttgggaagaaggaacaagacaaagctcacgcaactttgatacgcacgaaaattacatacaagatgacgatttccctcttgatgatgctggaactattggttgggatctagggattaccgaaaaaaatgaccagaacaacgatgatgacgataactctgttgagcaaggaagaaggctaggtgaatcaatcatgtctgaggagcctacggattttggatttgatctggatatagaaaaagaagcacccgcaggcaatatagatacgataaccgatgccatgacagaatcacagccaaaacaaactggcacaagaaggaactcaaaattactgaataccaaaagtattcaaatcgatgaagaaacagaaaattcagaaagcatagcttcatccaatacatacaaagaggagagatcgaataatttattgactccacagcccaccaattttacgactaaaagattatggagtgaaataactgaaagtatgtcctatttaccagatccaatccttaagaacttcttatcctacgaatctctaaaaaaaagaaaaatacataatgggcgagaagggtcaattgaagaacctgagttaaacgtgtcgcttaatcttaccgacgatgtaattagtaatgcgggaacaaacgataactccttcaatgaactgaccgataacatgtcagacttcgtaccaattgatgctggcttaaatgaggctccttttcctgaagagaatatcatcgacgcaaagactagaaatgagcaaactaccatccaaactgaaaaagttcgtcctactcccggtgaggtggcttctaaagctattgttcaaatggcgaagattttaaggaaggaattaagcgaagaaaaagaagtaatattcacggatgtactaaaaagtcaagcaaatacagaaccagaaaatattactaaaagagaagccagtagaggatttttcgatattttatcacttgctacagaaggatgtattggtttgagtcaaacagaagcattcggaaatattaaaatagacgccaaacctgcactatttgaaaggtttatcaatgcttaa

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