LOCUS       NM_001170447            5758 bp    mRNA    linear   VRT 29-JUN-2025
DEFINITION  Xenopus laevis dicer 1, ribonuclease III S homeolog (dicer1.S),
            mRNA.
ACCESSION   NM_001170447
VERSION     NM_001170447.1
KEYWORDS    RefSeq.
SOURCE      Xenopus laevis (African clawed frog)
  ORGANISM  Xenopus laevis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae;
            Xenopus; Xenopus.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from GQ485552.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ485552.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00012418, SAMD00012419
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Xenopus laevis"
                     /mol_type="mRNA"
                     /db_xref="taxon:8355"
                     /chromosome="8S"
                     /map="8S"
     gene            1..5758
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="dicer 1, ribonuclease III S homeolog"
                     /db_xref="GeneID:100316917"
                     /db_xref="Xenbase:XB-GENE-6460379"
     misc_feature    30..32
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="upstream in-frame stop codon"
     CDS             60..5447
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /EC_number="3.1.26.3"
                     /note="double-stranded RNA-specific endoribonuclease type
                     III; dicer 1, ribonuclease type III S homeolog"
                     /codon_start=1
                     /product="endoribonuclease Dicer-S"
                     /protein_id="NP_001163918.1"
                     /db_xref="GeneID:100316917"
                     /db_xref="Xenbase:XB-GENE-6460379"
                     /translation="

MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNIIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNAKRTVFLVNSEKQVSQQVSAVRTHTDLKVGEYSDLQKTQCWAKGKWYQEFQTHQVLVMTCHIFLNVLKTGNVSLSNINLLVFDECHLAIQDHPYRDIMKICESCQTCPRILGLTASILNGKCDPHDLEEKIQNLEEILRSNAETATDLVVLDRYASQPCEIVLDCGPYVDRSGLYQRLLNELDEALNFLTDCNISTHSKERDSTIISKQILSDCWAVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEDEEIEAKEKAETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSGKIKAFEEDLKTYKSIEKILRNKCSKSMDCGNTESEPIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGPYHSTLYLPINSPLRAPIVGPPMNCARLAERAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFSLNLEQLELITRLHQYIFSHILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDFKFVEDIEKSEARTGIPNTQYSAEGPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYDLDLTNLNQPLLDVDYTSSRLNLLTPRHLNQKGKALPLSSAERRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEELRAQTAIDAGVGVKSLPEDFRYPNLDFGWKRSIDGKTFISNQSFSAVERESDCRLSITTSPDSATSSAAHHVMYTQMNDQMSVNCTPPCPKSLSDLQGVCFSDDCKAINGITCNGLTNGNWEAESGACFQKDELIACKQEIPEKSTSFHVQNLPNDNQPILNECTLSKKFLDGNVGKPISDECPSTCTSDINYESGLSSGYSSKTLGPNPGLILQALTLSNASDGFNLERLEMPGDSFLKHAITTYLFCTYPDSHEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDKWESNETSGESVMANGKIDEDFDDEEDEDLMWRNPKEETDFDDDFLEYDQEHIKFIDSMLMGSGAFVKKIQLSAFAPPDQNYEWRPPKKPPLESAPFRSEFDDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENGGADAGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLDLKVLPEVRKPVTNTSTRGNYSLDGRTNSELSCKGIEYGYLKIPPRCMFEHPEAEKTLDHLISGFENFEKKINYQFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKTKCKGWILSYVGLKKTKRKKKILKSQKLWETYLSHLRELFIWTAACRWKLYGVFTIL"

     misc_feature    153..746
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="DEXH-box helicase domain of endoribonuclease Dicer;
                     Region: DEXHc_dicer; cd18034"
                     /db_xref="CDD:350792"
     misc_feature    order(153..164,171..173,222..245,366..368,555..557,
                     648..650)
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:350792"
     misc_feature    order(330..335,402..407,480..482,486..491,498..500,
                     573..581)
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="nucleic acid binding site [nucleotide binding];
                     other site"
                     /db_xref="CDD:350792"
     misc_feature    552..563
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="propagated from UniProtKB/Swiss-Prot
                     (A0A8J1LLF7.1); Region: DECH box.
                     /evidence=ECO:0000250|UniProtKB:B3DLA6"
     misc_feature    840..1124
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="Partner-binding domain of the endoribonuclease
                     Dicer; Region: Dicer_PBD; cd15903"
                     /db_xref="CDD:277191"
     misc_feature    order(855..860,867..869,873..878,1053..1058,1065..1070,
                     1077..1079,1086..1091,1098..1100)
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="Trbp binding interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:277191"
     misc_feature    1137..1727
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="C-terminal helicase domain of the endoribonuclease
                     Dicer; Region: SF2_C_dicer; cd18802"
                     /db_xref="CDD:350189"
     misc_feature    order(1380..1388,1596..1598,1653..1655,1659..1661)
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350189"
     misc_feature    order(1608..1610,1614..1616,1689..1691,1695..1697)
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:350189"
     misc_feature    1923..2189
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="Dicer dimerization domain; Region: Dicer_dimer;
                     pfam03368"
                     /db_xref="CDD:460900"
     misc_feature    2691..3059
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="PAZ domain, dicer_like subfamily. Dicer is an RNAse
                     involved in cleaving dsRNA in the RNA interference
                     pathway. It generates dsRNAs which are approximately 20 bp
                     long (siRNAs), which in turn target hydrolysis of
                     homologous RNAs. PAZ domains are named...; Region:
                     PAZ_dicer_like; cd02843"
                     /db_xref="CDD:239209"
     misc_feature    order(2880..2882,2919..2921,2937..2939,2949..2951,
                     3003..3005,3027..3029,3033..3035)
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239209"
     misc_feature    3876..>4151
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="Ribonuclease III family; Region: RIBOc; smart00535"
                     /db_xref="CDD:197778"
     misc_feature    order(3927..3932,3939..3944,3951..3953,3960..3965,
                     3972..3977,3981..3989,4017..4019)
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="dimerization interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:238333"
     misc_feature    4980..>5237
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="Ribonuclease III C terminal domain. This group
                     consists of eukaryotic, bacterial and archeal ribonuclease
                     III (RNAse III) proteins. RNAse III is a double stranded
                     RNA-specific endonuclease. Prokaryotic RNAse III is
                     important in post-transcriptional...; Region: RIBOc;
                     cd00593"
                     /db_xref="CDD:238333"
     misc_feature    order(5040..5045,5052..5057,5064..5066,5073..5078,
                     5085..5090,5094..5102,5130..5132)
                     /gene="dicer1.S"
                     /gene_synonym="dcr1; dicer; dicer1; herna"
                     /note="dimerization interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:238333"
ORIGIN      

ggttacaatgatttaagtcactggatttatgaaagccctgcattttgcagaccctcagcatggcaggccttcagctcatgactccagcctcctctccaatgggtcccttttttgggctgccctggcaacaggaggctattcatgacaacatttataccccacggaaatatcaggtggaactactcgaagcagcattggatcataatattatagtatgtttgaattctggctcagggaagacatttattgccgtactactcagtaaagagctgtcgtaccagatccgtggggacttcagcaaaaatgccaagaggactgtgtttttagtcaattctgagaaacaggtgtctcaacaagtgtctgctgtgaggacccatacagacctcaaggtgggagaatattcagatctacaaaaaacacaatgctgggcgaaaggaaaatggtaccaggaatttcagactcatcaggtcttggtcatgacctgccatatcttcctgaatgttctgaaaactgggaatgtgtcattgtcaaacattaatctattagtgtttgacgaatgtcatctggcaattcaagatcacccatatcgagacatcatgaagatatgtgaaagttgccagacatgccctcggatcctcggtctaactgcttcaatactaaatggaaaatgtgaccctcatgaccttgaggaaaagatccagaatctagaggaaatattaagaagtaatgcagaaactgcaactgatttggttgttttagacaggtatgcttcccaaccatgtgaaattgtattggactgtgggccgtacgttgatagaagtggactgtatcaaagacttttaaatgaattggatgaagcgcttaactttctcaccgactgtaatatttctacacattctaaagagagagattccacaataatttcaaaacagattttgtccgactgttgggctgttctgttggtcttgggaccatggtgtgctgataaagttgcagggatgatggtgagagagctgcagaagtatatcaaacatgagcaggaagaactgcacagaaaattccttttgtttacagacactatcttaaggaagatccacgctctttgtgaggaacacttctcaccagcctctcttgatatgaagtttgtcacacctaaagttataaaactgctggaaatcctgcgcaagtacaaaccctatgaacggcagcaatttgaaagcgttgaatggtataacaatagaaaccaggataactacgtatcttggagtgattcagaggatgatgatgatgatgaagatgaagaaatcgaggcgaaggaaaaagctgaaacaagctttccatccccattcacaaacatcttgtgtggcatcatctttgtggaacgaagatacacagccgtagtattaaacaggttgattaaagaagccgggaagcaagatccagagctggcctatattagtagcaactttattacaggacatggcataggaaagaaccagccacgcaataagcagatggaagttgagttcagaaagcaggaagaggttcttcgtaaatttcgtgcacacgaaaccaacctattgatagctacaagcattgttgaggaaggagtggacataccaaaatgcaacttagtggttcgatttgatttaccttcagagtaccgatcctatgttcagtccaaaggcagagctagagcaccaatctcaaattacatcatgctagcggatagtggtaaaataaaggcatttgaagaggaccttaaaacatacaaatcaattgaaaagattttgcggaacaaatgctcaaagtccatggattgtggaaatacagaatctgagcccattgtggatgatgatgaaatatttccaccttatgtgttgagacaagatgatggcagcccacgagttacaatcaacaccgctattggacacatcaacaggtactgtgctaggctacctagcgacccatttactcaccttgcgcccaagtgcagaacacgagagttgcctgatggaccttatcactcaacactctacctgccaattaattctccacttagagcccccattgttggtcctccaatgaattgtgcaaggctagctgagagagctgtagctcttatatgctgtaagaaactacatgaaataggtgaactggatgatcatttaatgccagttggcaaggaaactgtaaaatatgaggaggagcttgacttgcatgatgaggaagaaaccagtgtcccaggcagacctggatctacaaaaagaaggcagtgctatccaaaagctatccctgaatgtttacggaacagctacccgaagcccggtcagccttgttacttgtatgtaataggaatggtgttaaccactcctctaccagatgaacttaattttaggcgacggaagctgtatccccctgaagacacaacaagatgttttggaatacttactgccaaacccatacctcagattcctcactttcctgtctatactcgctcaggagaggtgaccatatcaattgaactgaagaagtctggtttttcattaaacttagaacaacttgagcttattactagactccaccagtacattttttctcatattcttcgtcttgagaaacctgcactagaatttaaacccacagtagccgattgtgcctactgtgttctacctcttaatgttgtaaatgattctggcactttggacattgactttaaatttgtagaagatattgagaaatccgaagcacgtactggtatacctaatacacagtattctgctgaaggtccttttattttcaaattagaagactaccaggatgcagttatcattccaagatatcgaaactttgatcagccgcacagattttatgtagctgatgtatacactgatcttacacctctcagcaagtttccttcccctgaatatgaaacgtttgctgaatattacaaaacaaagtatgacctcgatctcacaaacctcaatcagccacttttggatgtggactacacctcttcaagacttaatttgctgactcctcgacatctgaaccagaaggggaaagctctcccattaagcagtgctgaaaggagaaaagccaaatgggagagcttacaaaacaaacagatcctggttccagagctttgtgctatacatccagtcccagcatccttatggagaaaagctgtttgtttgccaagcatactttatcgtttgcattgcctccttacagcagaagagttgagagcccaaacagctattgatgctggagtaggagtcaaatcgcttcctgaagatttccgatacccaaatttggattttggatggaaaaggtctatagacggtaaaacattcatctctaatcaaagcttctcagcagtcgagcgtgaaagtgactgcagactctctataaccacaagccctgacagtgctacaagctcagctgctcatcatgtaatgtacacgcaaatgaatgaccaaatgtctgtgaactgcacaccgccatgtccgaaatctctcagtgacctccaaggggtttgcttctcagacgattgcaaagcaattaacggtattacttgcaatggtctgaccaatggcaattgggaagcagaaagcggtgcgtgttttcagaaggatgaactaattgcttgcaaacaggaaatacctgaaaaatctacctcgtttcatgttcagaatttacctaacgacaaccagcccatacttaatgaatgtactctgagcaaaaagtttcttgatgggaatgtcggcaaacctatctcggatgagtgccctagcacctgtacctcagacattaattatgaaagtgggctttccagtgggtactcttcaaaaactcttggcccaaaccctggtcttatcctccaggcgttgactttgtccaatgcaagtgatggtttcaatctggagcgtctcgaaatgcctggtgactcattcctgaaacatgccattaccacatatctgttctgcacataccccgattctcacgagggtcgcctgtcctatatgcgcagcaagaaggtcagtaactgtaacctatatcgacttggaaaaaagaaaggctcacccagccggatggtggtgtccattttcgatccacctgtcaactggcttccccctggttatatcgttaaccaggacaagaattctgataagtgggaaagtaatgaaacgtctggtgagagtgtgatggcaaatggcaaaattgatgaagactttgatgatgaagaagatgaagatcttatgtggaggaatccaaaagaggaaactgattttgatgatgactttctagaatatgaccaagagcatataaaattcattgacagcatgttaatgggatctggggcatttgtgaagaaaatacagctttctgcttttgcaccccctgatcagaattatgaatggaggccgccaaagaaacctccactggaaagtgctccgttccgctctgaatttgatgattttgattacagttcatgggatgccatgtgttatttggatcccagcaaggctgtagaggaggatgactttgtagtaggcttttggaatccatcagaagagaacggtggagctgatgctggaaagcagtccatttcttatgacctacacacagagcaatgtattgcagacaaaagtattgctgactgtgtagaggctttattgggctgctatctgaccagttgtggtgagagagctgcacaacttttcctttgttctttggacttaaaagtactcccagaagtaagaaagcctgttactaacacaagtaccagaggcaactatagtcttgatggcagaacaaactctgagctgtcctgcaaaggaatagaatacggttatttgaagatccctccgaggtgcatgtttgagcacccagaagctgaaaaaacccttgaccatctaatatctggttttgagaactttgagaagaaaataaactatcagtttaaaaacaaggcttaccttctacaggcttttacacatgcctcttaccactacaatactataactgattgctaccagcgtttagagtttcttggagatgcaattttggactacctcattactaagcacctttatgaagacccacgccagcactcgccaggagtcctcactgacctgcgttctgctctggtgaataacaccatatttgcatcactagctgtgaaatatgactaccataaatactttaaggccatctctcctgaactgttccatgtaattgatgattttgtgcaatttcaacttgaaaaaacgaaatgcaagggatggattctgagttacgtaggtctgaagaagacgaagagaaagaagaagatattgaagtcccaaaagctatgggagacatatttgagtcacttgcgggagctatttatatggacagcggcatgtcgttggaaactgtatggcgtgtttactatcctatgatgcagccgctaattgaaaaattctctgctaatgtccctcgttccccggtgagagaattgctggaaatggagcctgaaactgctaaattcagtccggccgaaagaacatacgatggaaaagtcagagtaatggtggaagttgttggaaaaggaaaatttaaaggggtcggaagaagttacagaattgccaaatctgcagcagccaggagagcactaagaagcctcaaagctaatcaatctcaggtccctaacagctgagtattcctgcacttcactgaagggaatttatccaaagctgagctctatcctcca

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