LOCUS       NM_001096105            4129 bp    mRNA    linear   VRT 27-APR-2025
DEFINITION  Xenopus laevis argonaute 4, RISC catalytic component L homeolog
            (ago4.L), mRNA.
ACCESSION   NM_001096105
VERSION     NM_001096105.1
KEYWORDS    RefSeq.
SOURCE      Xenopus laevis (African clawed frog)
  ORGANISM  Xenopus laevis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae;
            Xenopus; Xenopus.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Klein,S.L., Strausberg,R.L., Wagner,L., Pontius,J., Clifton,S.W.
            and Richardson,P.
  TITLE     Genetic and genomic tools for Xenopus research: The NIH Xenopus
            initiative
  JOURNAL   Dev Dyn 225 (4), 384-391 (2002)
   PUBMED   12454917
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC098982.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098982.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN04111056, SAMN04111070
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Xenopus laevis"
                     /mol_type="mRNA"
                     /db_xref="taxon:8355"
                     /chromosome="2L"
                     /map="2L"
     gene            1..4129
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /note="argonaute 4, RISC catalytic component L homeolog"
                     /db_xref="GeneID:734630"
                     /db_xref="Xenbase:XB-GENE-1006885"
     exon            1..105
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..2675
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /note="argonaute RISC catalytic component 4"
                     /codon_start=1
                     /product="protein argonaute-4"
                     /protein_id="NP_001089574.1"
                     /db_xref="GeneID:734630"
                     /db_xref="Xenbase:XB-GENE-1006885"
                     /translation="

MGRKTLCESETVCRDVTINGSAGCLQNVGPPPTNLFQPPRRPGLGTLGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMPIFGDNQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVTIQWVSVVSLQLLLEALSGHLSEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDVQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKLTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQETTQELLYSQEVIQDLCNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELMAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHSMYFA"

     misc_feature    165..554
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    585..737
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    738..1100
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(894..896,939..941,981..983,993..995,1047..1049,
                     1068..1070,1074..1076)
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1239..2546
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1650..1652,1662..1664,1698..1709,1716..1718,
                     1740..1742,1749..1751,1761..1763,1773..1775)
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1854..1856,1860..1862,2100..2102,2514..2516)
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2562..2630
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q4KLV6.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     exon            106..274
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            275..395
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            396..577
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            578..714
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            715..849
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            850..937
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1085
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1205
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1334
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1468
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1653
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1813
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..2129
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2264
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2366
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2367..2566
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2567..4079
                     /gene="ago4.L"
                     /gene_synonym="ago4; argonaute-4; Argonaute4; eif2c4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ttttaaggcagaaggagagaatgggcagaaaaactttgtgcgaaagtgaaacagtctgcagggatgtcacaataaatggaagtgctgggtgcctgcaaaatgttggccctccaccgactaacctcttccagcctccgcggcgtcctggcctgggaacactgggaaagccaattaggctactggccaaccattttcaggttcagatcccgaagatcgatgtctaccattatgatgtggacatcaagccagagaaaaggccaaggagagtcaacagggaggtggtggatacaatggtacgtcatttcaagatgccgatttttggagacaaccagccaggttatgatggtaaaaggaacatgtacactgctcacccactaccaattggcagagacagagttgatttggaagtgacccttcctggtgaaggcaaagaccagactttcaaagtcactattcagtgggtctcggtagtgagtcttcagcttcttttagaggctctctctggacatctaagtgaagttccggatgattctgttcaggcgctagatgtaatcactcgccatctaccatccatgaggtatacaccagttggacggtccttcttctctcctcctgaaggttattatcacccactcggtggtggccgagaagtttggtttggctttcaccagtctgttcgtccagctatgtggaacatgatgctcaatattgatgtttctgcaacagctttttaccgagctcagccggtcatagaatttatgtgtgaagtcctagacgtgcaaaacatcaatgagcaaacaaagcctctgactgattcacagcgtgtcaagttcaccaaagaaataagaggtctgaaagttgaagtgacacactgtggacagatgaaaagaaaatacagagtgtgcaatgttactagaaggccagcaagccaccagacgtttcctttacagttggagaatggtcaagccatggagtgtactgttgctcagtactttaagcagaaatatagcttacaattaaaatatccccatctcccttgcttgcaagttggacaggagcagaaacacacatacttaccacttgaggtttgcaacattgtagctggccaacgatgtattaagaaactgacagacaatcagacttccactatgatcaaagctaccgcccgatctgctccagacagacaggaggagatcagcaggctggtgaagagtaacagtatggttggtggaccagatccgtacctcaaggagtttggcattgttgttcataatgaaatgacagagctaacaggcagagtgttgccagcaccaatgcttcagtatggaggaaggaataaaactgtggccaccccaaaccagggtgtttgggacatgagaggaaagcagttctatgctggtattgagatcaaagtttgggctgttgcttgttttgcaccacagaagcagtgccgagaagacttgttaaagagtttcactgatcagttaagaaagatctccaaagatgcaggaatgcccattcaaggtcagccttgcttctgtaaatatgctcaaggcgctgacagtgtggagccaatgttcaaacatcttaagttgacatatgttggtcttcaacttatcgtggtgattttgcctggcaagactccagtttatgcggaagtgaaacgagtaggagacacgctacttggtatggccacccagtgtgttcaggtgaaaaatgtagtcaagacatctcctcagacactatcaaacctctgcctgaaaatcaatgccaagctagggggaattaacaatgtgttggtgcctcaccaaagaccttcagtgtttcagcagcctgttatatttttgggagctgatgtaacacacccccctgctggggatggcaagaaaccttccattgcagctgtagtgggcagcatggatgggcatcccagccgctactgtgccactgtgcgtgttcagacctcacgccaagagacaacccaagagttgctgtacagccaggaagttattcaagatctttgcaacatggttcgggaactgcttatccagttttacaagtcaactcgctttaaaccaacaagaatcatctactatagaggaggcgtttctgaaggtcaaatgaaacaggtggcatggccagagcttatggcaattcgaaaggcctgcatcagcttggaagaagattacagacctggaataacctacattgttgtacaaaagaggcatcatacaaggcttttttgctctgataaaacagaaagggttggaaagagtggcaatgtgccagcaggaactacagtggacagcacaataacacacccatcagaatttgatttctatctctgtagtcatgctgggattcagggaacaagtcgtccatctcattaccaggttctctgggatgataactgcttcactgctgatgaactccagctactaacctaccagctgtgtcacacatatgtgcggtgcacgcggtctgtttcaatccccgcccctgcttactatgctcgactggttgcattcagagcaaggtatcatcttgtagataaggatcatgacagtgctgaaggaagccatgtatctggtcagagcaatggtcgagatcctcaagctctggccaaagctgttcagattcaccacgacacacaacactcaatgtactttgcctgacagcacagtgagaactggcacagactctcctcatccttcttttccaggccctgaagtcttctgttaagcaggcttgcctgtaattttcagggccagttctgtctcttggcttcccaccagcacattttggactttttgcactgaatctctttaccatggcatttgaatggaggaaagatgcaaacctttttatttagttatctcaaaaaacttatggattgtcttctgatgcattggactgaatatttacctcaagctcaaaagatatctgaacattttactccaagcaactcttgttgtgttaattgttttaatttgcaagccagcatttcttcatccttggttgtaacaaaaatgtacatgctttttgtctgaacatgctgctttgcaactgtacttaaatggaaaagtttaactgagtgggccatagacttgctaaatgaggtttagtccttactgtataataacttcttaatgtttttacagataatgccgtctgtcaggttcttaaccattgcaaacttgtactttttaacgagtgcaggctgcaaacctattgcactatattgcatattaattaaggtaagtttttatttctgtataaaatgaatgtgtaatattaagatttaatataagggccatgtggcgagcactgccatatagttttttcacttgcagattgccattacttttccttccccaggcacttttgcttcccaagacaaccgtatgttagtattttgccagcgcacacagacgcccgtgtcaatgctgctgcagtttgataaaagtttttactctcggagggatgggtgtggggtaccatttttatggcaataaaacattttagtatcataacatcatttcaacatccacctggtttttgggtgttgtatggtgtagacttttttctcctttcagtaaaatttgtcaaattgattttacaatcactttttcacatggtttttttttttcctaatggagattgttacttccagtaaaggttatgacctcttgactattctggaacccttggcagagaattctttattgccagcagggcaggccctgcatgtgcaaaaatcaaactcctttacttttgggagagatgtaggcagctttggagggcagagactacacgatttataccagcattttccgtttgtgttggtcctctgaacccagactaacaaagtgcatgtttgtataatttgcatgtacataattttcttattataccttttagtttatcatttttatgaatgaaaactgaaccaattctatatacacatgctctttgctatcctccttgctctctcaactataaaaggtaattgagataaacctttacagtgctctactacatgcaggttttctgatgtagcgactccattaactttaacataaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa

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