LOCUS       XM_053453123            1382 bp    mRNA    linear   VRT 04-MAR-2023
DEFINITION  PREDICTED: Spea bombifrons homeobox B8 (HOXB8), transcript variant
            X2, mRNA.
ACCESSION   XM_053453123
VERSION     XM_053453123.1
DBLINK      BioProject: PRJNA930407
KEYWORDS    RefSeq.
SOURCE      Spea bombifrons (plains spadefoot toad)
  ORGANISM  Spea bombifrons
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Pelobatoidea; Pelobatidae; Spea.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_071099) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_027358695.1-RS_2023_03
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 03/03/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Spea bombifrons"
                     /mol_type="mRNA"
                     /isolate="aSpeBom1"
                     /db_xref="taxon:233779"
                     /chromosome="13"
                     /sex="female"
                     /tissue_type="muscle"
                     /dev_stage="adult"
                     /country="USA: Anton, Colorado"
                     /lat_lon="39.74511 N 103.19081 W"
                     /collection_date="2019-07-01"
                     /collected_by="Catherine Chen"
     gene            1..1382
                     /gene="HOXB8"
                     /note="homeobox B8; Derived by automated computational
                     analysis using gene prediction method: Gnomon. Supporting
                     evidence includes similarity to: 21 Proteins"
                     /db_xref="GeneID:128471235"
     CDS             1..729
                     /gene="HOXB8"
                     /codon_start=1
                     /product="homeobox protein Hox-B8 isoform X2"
                     /protein_id="XP_053309098.1"
                     /db_xref="GeneID:128471235"
                     /translation="

MSSYFVNSLFSKYKTGESLRPNYYDCGFAQDLGGRPTVVYGPSAGGTFQHPTQIQEFYHGTSSLSSPPYQQNPCAVTCHGDPSSFYGYDHLQRQSLFSTQDSDLVQYADCKLASAGLGEEAESSEQSPSPTQLFPWMRPQAAGRRRGRQTYSRYQTLELEKEFLFNPYLTRKRRIEVSHALGLTERQVKIWFQNRRMKWKKENNKDKFPSSKCEQEEMEKQKMERAQDTEEGMDGQKGEKSN"

     misc_feature    order(433..447,451..453,502..504,520..522,559..561,
                     565..570,577..582,586..594,598..603)
                     /gene="HOXB8"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     misc_feature    order(439..441,448..450,568..570,577..582,589..591)
                     /gene="HOXB8"
                     /note="specific DNA base contacts [nucleotide binding];
                     other site"
                     /db_xref="CDD:238039"
     misc_feature    442..600
                     /gene="HOXB8"
                     /note="Homeobox domain; Region: Homeobox; pfam00046"
                     /db_xref="CDD:425441"
     polyA_site      1382
                     /gene="HOXB8"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

atgagctcttattttgtcaattcactcttctccaaatacaaaactggggaatctttgcgccccaattactatgattgtgggtttgctcaggatctaggaggtagacccacagttgtctatggacccagtgctgggggaacctttcagcatcctacccaaattcaggagttttaccacggaacatcctctctgtcctcacccccttaccagcaaaatccctgcgctgtgacctgccacggggatccaagcagcttctatggatacgaccatttacaaagacagagcctgttcagcacccaggactcggacttagtccaatacgctgactgcaagctggcctcagctggcctgggggaagaagcagaaagctctgagcagagcccctctcccacgcagctctttccgtggatgagaccacaagctgccggccgtaggagaggaaggcagacttacagccgctaccagaccctggaactggagaaagaattcctcttcaatccctacctgacgcgcaaaaggcgcattgaggtatctcatgctctaggactcaccgagagacaggtcaaaatctggttccagaacaggaggatgaaatggaaaaaagaaaataacaaggacaagttccccagcagcaaatgtgagcaggaagaaatggagaaacagaaaatggagagggcgcaggacacagaggaagggatggatggacaaaagggggaaaagagtaattaatgtggactttaagccatcgaggaggcttccttattgttgtcctaccttccatcaaataatacaggagggagacacaatgcccctggatgagatccggccctaacccctctctgtaacccatcttatttgatacataaattaccagtgccccttaagacatgatgtcttcctatctctggtgggggtgggtcagcgtggacaaaatgactttctagtgtttgtgtttaacgcaatagcttttgtggatcttaacttggggtttatttggtcgggaaaacgtttgctatagaagttttgtgtgcactatcctgtttgtggtaactgtatttctttatatatgtacagatatatatatatataaaagaaagaaaccaacaacatgaaactgcctaattgagctgtaggtccctctaacacattagatccccatctttgttttgtccccagtgtttgcaggatttgagttagcatgcgcgcagttgtggtattagggttcctgtgagcagactgtatactgtatatgtgaataccttttttatgaggggtgggggttgattctttattttgtttgttctgcacactttgttctattgtctaaaccatgaagcggcggaagaagacctgtaataaaagtttacaagagggagtcgtga

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