LOCUS       XM_013831861             726 bp    mRNA    linear   PLN 23-JUN-2022
DEFINITION  PREDICTED: Brassica napus autophagy-related protein 8c
            (LOC106391255), transcript variant X4, mRNA.
ACCESSION   XM_013831861
VERSION     XM_013831861.3
DBLINK      BioProject: PRJNA844685
KEYWORDS    RefSeq.
SOURCE      Brassica napus (rape)
  ORGANISM  Brassica napus
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Brassiceae; Brassica.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_063447) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Jun 23, 2022 this sequence version replaced XM_013831861.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Brassica napus Annotation Release
                                           102
            Annotation Version          :: 102
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 9.0
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Brassica napus"
                     /mol_type="mRNA"
                     /cultivar="Da-Ae"
                     /db_xref="taxon:3708"
                     /chromosome="C4"
                     /tissue_type="Seedling"
                     /dev_stage="Seedling"
                     /country="USA"
     gene            1..726
                     /gene="LOC106391255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 18 long SRA reads, 26 Proteins,
                     and 100% coverage of the annotated genomic feature by
                     RNAseq alignments, including 150 samples with support for
                     all annotated introns"
                     /db_xref="GeneID:106391255"
     CDS             256..615
                     /gene="LOC106391255"
                     /codon_start=1
                     /product="autophagy-related protein 8c isoform X2"
                     /protein_id="XP_013687315.2"
                     /db_xref="GeneID:106391255"
                     /translation="

MADISFKSEHPLERRQIEASRIRDKYPDRVPVIVEKAERSDVPNIDKKKFLVPADLTVGQFVYVVRKRIKLSAEKAIFVFVKNTLPQTAAMMSAVSDENKDEDGFLYMTYSGENTFGLV"

     misc_feature    289..597
                     /gene="LOC106391255"
                     /note="ubiquitin-like (Ubl) domain found in Saccharomyces
                     cerevisiae Atg8p and related proteins; sub-family of the
                     autophagy-related 8 (ATG8) family; Region: Ubl_ATG8;
                     cd16128"
                     /db_xref="CDD:340545"
     misc_feature    order(307..309,316..321,340..342,382..396,400..414,
                     421..423,430..438,442..447,454..465,472..474,568..570)
                     /gene="LOC106391255"
                     /note="Atg19 interaction site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(307..309,319..321,340..342,346..354,394..396,
                     400..408,412..414,445..447,457..459,568..570)
                     /gene="LOC106391255"
                     /note="peptide binding site 2 [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(307..309,340..342,346..348,394..396,400..408,
                     412..414,436..438,445..447,457..459,568..570)
                     /gene="LOC106391255"
                     /note="peptide binding site 1 [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(340..342,367..372,394..396,403..405,430..435,
                     439..447,451..459,472..477,481..489,493..495,502..510,
                     514..522,592..597)
                     /gene="LOC106391255"
                     /note="Atg7 interaction site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(364..366,439..441,451..453,472..489,493..495,
                     502..519,580..582,586..597)
                     /gene="LOC106391255"
                     /note="Atg4 interaction site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    457..459
                     /gene="LOC106391255"
                     /note="key conserved lysine K33; other site"
                     /db_xref="CDD:340545"
     polyA_site      726
                     /gene="LOC106391255"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

tacacctttccgggttacccaatagaacaaatggcaaaactaaaaagtttccaatgacgacgtgtcgataactaaaaaagatcaaagtttcatcctttcagagtttatcagcttcgcgcagcgattacaaatattcttcaaaccctaattgagttgtcttcttctatttcgacaatctaaattggagaacgagaaaccctaatcttagatttcaattggtgttgattgatttcaggaattcgattctcaatcgccatggctgatatctctttcaagtcggaacacccgctggagaggagacagattgaagcttctcgcatcagggacaagtatccagacagagttccagtgattgtagagaaagctgagagaagtgatgttcccaatatcgacaagaaaaagttccttgtaccggctgatctaaccgtggggcaatttgtgtatgttgtccgtaaaagaatcaagctgagtgcagaaaaggcaatctttgtctttgtcaagaacacattgcctcaaactgctgcaatgatgtctgcagtctctgatgagaacaaagacgaagatgggtttctctacatgacttatagtggagagaacacttttggtttggtttaaaatctgtctacttttgaagtgttttcatgtacatatatgctatgctctttgaatatggagaatttttttatgttgctttggactgattatatgtaaattttgctttggatt

//