LOCUS       XM_013778509             662 bp    mRNA    linear   PLN 25-AUG-2015
DEFINITION  PREDICTED: Brassica oleracea var. oleracea autophagy-related
            protein 8c (LOC106339658), transcript variant X4, mRNA.
ACCESSION   XM_013778509
VERSION     XM_013778509.1
DBLINK      BioProject: PRJNA293438
KEYWORDS    RefSeq.
SOURCE      Brassica oleracea var. oleracea
  ORGANISM  Brassica oleracea var. oleracea
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Brassiceae; Brassica.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_013617409.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Brassica oleracea Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 6.4
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Brassica oleracea var. oleracea"
                     /mol_type="mRNA"
                     /variety="oleracea"
                     /cultivar="TO1000"
                     /db_xref="taxon:109376"
                     /chromosome="C4"
                     /country="Canada"
                     /lat_lon="52.1333 N 106.6833 W"
                     /collection_date="31-Aug-2009"
     gene            1..662
                     /gene="LOC106339658"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 1 EST, 27 Proteins, and 100%
                     coverage of the annotated genomic feature by RNAseq
                     alignments, including 27 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:106339658"
     CDS             214..573
                     /gene="LOC106339658"
                     /codon_start=1
                     /product="autophagy-related protein 8c isoform X2"
                     /protein_id="XP_013633963.1"
                     /db_xref="GeneID:106339658"
                     /translation="

MADISFKSEHPLERRQIEASRIRDKYPDRVPVIVEKAERSDVPNIDKKKFLVPADLTVGQFLYVVRKRIKLSAEKAIFVFVKNTLPQTAAMMSAVSDENKDEDGFLYMTYSGENTFGLV"

     misc_feature    247..555
                     /gene="LOC106339658"
                     /note="ubiquitin-like (Ubl) domain found in Saccharomyces
                     cerevisiae Atg8p and related proteins; sub-family of the
                     autophagy-related 8 (ATG8) family; Region: Ubl_ATG8;
                     cd16128"
                     /db_xref="CDD:340545"
     misc_feature    order(265..267,274..279,298..300,340..354,358..372,
                     379..381,388..396,400..405,412..423,430..432,526..528)
                     /gene="LOC106339658"
                     /note="Atg19 interaction site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(265..267,277..279,298..300,304..312,352..354,
                     358..366,370..372,403..405,415..417,526..528)
                     /gene="LOC106339658"
                     /note="peptide binding site 2 [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(265..267,298..300,304..306,352..354,358..366,
                     370..372,394..396,403..405,415..417,526..528)
                     /gene="LOC106339658"
                     /note="peptide binding site 1 [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(298..300,325..330,352..354,361..363,388..393,
                     397..405,409..417,430..435,439..447,451..453,460..468,
                     472..480,550..555)
                     /gene="LOC106339658"
                     /note="Atg7 interaction site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(322..324,397..399,409..411,430..447,451..453,
                     460..477,538..540,544..555)
                     /gene="LOC106339658"
                     /note="Atg4 interaction site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    415..417
                     /gene="LOC106339658"
                     /note="key conserved lysine K33; other site"
                     /db_xref="CDD:340545"
ORIGIN      

tacccaatagaacaaatggcaaaactaaaaagtttccaatgacgacgtgtcgataactaaaaaggatcaaagttccatcctttcagagtttatcagcttcgcgcagagattacaaatattcttcaaaccctaatcgagttgtcttcttctatttcgacaatctaaattggagaacgagaaaccgtaatcttaggaattcgattctcaatcgccatggctgatatctctttcaagtcggaacacccgctggagaggagacagattgaagcttctcgcatcagggacaagtatccagacagagttccagtgattgtagagaaagctgagagaagtgatgttcccaatatcgacaagaaaaagttccttgtaccggctgatctaaccgtggggcaatttttgtatgttgtccgtaaaagaatcaagctgagtgcagaaaaggcaatctttgtctttgtcaagaacacattgcctcaaactgctgcaatgatgtctgcagtctctgatgagaacaaagacgaagatgggtttctctacatgacttatagtggagagaacacttttggtttggtttaaaatctgtctacttttgaagtgttttcatgtacatatatgctatgctctttgaatatggagaatttttttatgttgctttggactgatta

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