LOCUS       XM_013778508             668 bp    mRNA    linear   PLN 25-AUG-2015
DEFINITION  PREDICTED: Brassica oleracea var. oleracea autophagy-related
            protein 8c (LOC106339658), transcript variant X3, mRNA.
ACCESSION   XM_013778508
VERSION     XM_013778508.1
DBLINK      BioProject: PRJNA293438
KEYWORDS    RefSeq.
SOURCE      Brassica oleracea var. oleracea
  ORGANISM  Brassica oleracea var. oleracea
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Brassiceae; Brassica.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_013617409.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Brassica oleracea Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 6.4
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Brassica oleracea var. oleracea"
                     /mol_type="mRNA"
                     /variety="oleracea"
                     /cultivar="TO1000"
                     /db_xref="taxon:109376"
                     /chromosome="C4"
                     /country="Canada"
                     /lat_lon="52.1333 N 106.6833 W"
                     /collection_date="31-Aug-2009"
     gene            1..668
                     /gene="LOC106339658"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 1 EST, 28 Proteins, and 100%
                     coverage of the annotated genomic feature by RNAseq
                     alignments, including 27 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:106339658"
     CDS             220..579
                     /gene="LOC106339658"
                     /codon_start=1
                     /product="autophagy-related protein 8c isoform X2"
                     /protein_id="XP_013633962.1"
                     /db_xref="GeneID:106339658"
                     /translation="

MADISFKSEHPLERRQIEASRIRDKYPDRVPVIVEKAERSDVPNIDKKKFLVPADLTVGQFLYVVRKRIKLSAEKAIFVFVKNTLPQTAAMMSAVSDENKDEDGFLYMTYSGENTFGLV"

     misc_feature    253..561
                     /gene="LOC106339658"
                     /note="ubiquitin-like (Ubl) domain found in Saccharomyces
                     cerevisiae Atg8p and related proteins; sub-family of the
                     autophagy-related 8 (ATG8) family; Region: Ubl_ATG8;
                     cd16128"
                     /db_xref="CDD:340545"
     misc_feature    order(271..273,280..285,304..306,346..360,364..378,
                     385..387,394..402,406..411,418..429,436..438,532..534)
                     /gene="LOC106339658"
                     /note="Atg19 interaction site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(271..273,283..285,304..306,310..318,358..360,
                     364..372,376..378,409..411,421..423,532..534)
                     /gene="LOC106339658"
                     /note="peptide binding site 2 [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(271..273,304..306,310..312,358..360,364..372,
                     376..378,400..402,409..411,421..423,532..534)
                     /gene="LOC106339658"
                     /note="peptide binding site 1 [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(304..306,331..336,358..360,367..369,394..399,
                     403..411,415..423,436..441,445..453,457..459,466..474,
                     478..486,556..561)
                     /gene="LOC106339658"
                     /note="Atg7 interaction site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    order(328..330,403..405,415..417,436..453,457..459,
                     466..483,544..546,550..561)
                     /gene="LOC106339658"
                     /note="Atg4 interaction site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340545"
     misc_feature    421..423
                     /gene="LOC106339658"
                     /note="key conserved lysine K33; other site"
                     /db_xref="CDD:340545"
ORIGIN      

aaactaaaaagtttccaatgacgacgtgtcgataactaaaaaggatcaaagttccatcctttcagagtttatcagcttcgcgcagagattacaaatattcttcaaaccctaatcgagttgtcttcttctatttcgacaatctaaattggagaacgagaaaccgtaatcttagatttcaactggtgttgattggtttcaggaattcgattctcaatcgccatggctgatatctctttcaagtcggaacacccgctggagaggagacagattgaagcttctcgcatcagggacaagtatccagacagagttccagtgattgtagagaaagctgagagaagtgatgttcccaatatcgacaagaaaaagttccttgtaccggctgatctaaccgtggggcaatttttgtatgttgtccgtaaaagaatcaagctgagtgcagaaaaggcaatctttgtctttgtcaagaacacattgcctcaaactgctgcaatgatgtctgcagtctctgatgagaacaaagacgaagatgggtttctctacatgacttatagtggagagaacacttttggtttggtttaaaatctgtctacttttgaagtgttttcatgtacatatatgctatgctctttgaatatggagaatttttttatgttgctttggactgatta

//