LOCUS       NM_203359               5828 bp    mRNA    linear   MAM 19-DEC-2022
DEFINITION  Bos taurus dicer 1, ribonuclease III (DICER1), mRNA.
ACCESSION   NM_203359
VERSION     NM_203359.1
KEYWORDS    RefSeq.
SOURCE      Bos taurus (cattle)
  ORGANISM  Bos taurus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia;
            Pecora; Bovidae; Bovinae; Bos.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Burrola-Barraza,M.E., Hernandez-Seanez,R., Barcelo-Fimbres,M.,
            Rodriguez-Almeida,F.A., Gonzalez-Rodriguez,E., Garcia-Quinonez,S.,
            Grado-Ahuir,J.A. and Moreno-Brito,V.
  TITLE     Dicer gene expression during early bovine embryo development
  JOURNAL   Mol Reprod Dev 78 (9), 622 (2011)
   PUBMED   21823189
  REMARK    GeneRIF: Studied Dicer gene expression during early bovine embryo
            development.An increase in Dicer expression was detected in the
            mature oocytes sampled (P<0.05), implying that gene repression
            occurs during meiosis II.
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D,
            Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marcais G,
            Roberts M, Subramanian P, Yorke JA and Salzberg SL.
  TITLE     A whole-genome assembly of the domestic cow, Bos taurus
  JOURNAL   Genome Biol 10 (4), R42 (2009)
   PUBMED   19393038
REFERENCE   3  (bases 1 to 5828)
  AUTHORS   Ishiwata H, Katsuma S, Kizaki K, Patel OV, Nakano H, Takahashi T,
            Imai K, Hirasawa A, Shiojima S, Ikawa H, Suzuki Y, Tsujimoto G,
            Izaike Y, Todoroki J and Hashizume K.
  TITLE     Characterization of gene expression profiles in early bovine
            pregnancy using a custom cDNA microarray
  JOURNAL   Mol Reprod Dev 65 (1), 9-18 (2003)
   PUBMED   12658628
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY386968.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY386968.1, GGVG01110265.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMN03145412, SAMN03145413
                                           [ECO:0000350]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Bos taurus"
                     /mol_type="mRNA"
                     /db_xref="taxon:9913"
                     /chromosome="21"
                     /map="21"
     gene            1..5828
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="dicer 1, ribonuclease III"
                     /db_xref="BGD:BT29583"
                     /db_xref="GeneID:337871"
                     /db_xref="VGNC:VGNC:28063"
     misc_feature    14..16
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="upstream in-frame stop codon"
     CDS             17..5788
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /EC_number="3.1.26.3"
                     /note="Dicer1, Dcr-1 homolog; dicer 1, ribonuclease type
                     III; ribonuclease III"
                     /codon_start=1
                     /product="endoribonuclease Dicer"
                     /protein_id="NP_976235.1"
                     /db_xref="BGD:BT29583"
                     /db_xref="GeneID:337871"
                     /db_xref="VGNC:VGNC:28063"
                     /translation="

MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTVSLSALILDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNGLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPEADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPEITKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS"

     misc_feature    140..733
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="DEXH-box helicase domain of endoribonuclease Dicer;
                     Region: DEXHc_dicer; cd18034"
                     /db_xref="CDD:350792"
     misc_feature    order(140..151,158..160,209..232,353..355,542..544,
                     635..637)
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:350792"
     misc_feature    order(317..322,389..394,467..469,473..478,485..487,
                     560..568)
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="nucleic acid binding site [nucleotide binding];
                     other site"
                     /db_xref="CDD:350792"
     misc_feature    539..550
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6TUI4.3);
                     Region: DECH box"
     misc_feature    782..1801
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6TUI4.3);
                     Region: Required for interaction with PRKRA and TARBP2.
                     /evidence=ECO:0000250"
     misc_feature    827..1111
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Partner-binding domain of the endoribonuclease
                     Dicer; Region: Dicer_PBD; cd15903"
                     /db_xref="CDD:277191"
     misc_feature    order(842..847,854..856,860..865,1040..1045,1052..1057,
                     1064..1066,1073..1078,1085..1087)
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Trbp binding interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:277191"
     misc_feature    1130..1708
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="C-terminal helicase domain of the endoribonuclease
                     Dicer; Region: SF2_C_dicer; cd18802"
                     /db_xref="CDD:350189"
     misc_feature    1244..1315
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6TUI4.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1253..1255
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (Q6TUI4.3); phosphorylation site"
     misc_feature    1259..1261
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (Q6TUI4.3); phosphorylation site"
     misc_feature    order(1361..1369,1577..1579,1634..1636,1640..1642)
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350189"
     misc_feature    order(1589..1591,1595..1597,1670..1672,1676..1678)
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:350189"
     misc_feature    1901..2167
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Dicer dimerization domain; Region: Dicer_dimer;
                     pfam03368"
                     /db_xref="CDD:427264"
     misc_feature    2669..3037
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="PAZ domain, dicer_like subfamily. Dicer is an RNAse
                     involved in cleaving dsRNA in the RNA interference
                     pathway. It generates dsRNAs which are approximately 20 bp
                     long (siRNAs), which in turn target hydrolysis of
                     homologous RNAs. PAZ domains are named...; Region:
                     PAZ_dicer_like; cd02843"
                     /db_xref="CDD:239209"
     misc_feature    order(2858..2860,2897..2899,2915..2917,2927..2929,
                     2981..2983,3005..3007,3011..3013)
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239209"
     misc_feature    3059..3061
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (Q6TUI4.3); phosphorylation site"
     misc_feature    3494..3496
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (Q6TUI4.3); phosphorylation site"
     misc_feature    3752..3889
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6TUI4.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    3902..>4180
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Ribonuclease III family; Region: RIBOc; smart00535"
                     /db_xref="CDD:197778"
     misc_feature    order(3953..3958,3965..3970,3977..3979,3986..3991,
                     3998..4003,4010..4018,4046..4048)
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="dimerization interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:238333"
     misc_feature    4397..4399
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (Q6TUI4.3); phosphorylation site"
     misc_feature    4421..4423
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8R418; propagated from
                     UniProtKB/Swiss-Prot (Q6TUI4.3); phosphorylation site"
     misc_feature    4427..4429
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (Q6TUI4.3); phosphorylation site"
     misc_feature    5015..5773
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="dsRNA-specific ribonuclease [Transcription];
                     Region: Rnc; COG0571"
                     /db_xref="CDD:223644"
     misc_feature    5435..5437
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Important for activity. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q6TUI4.3); other
                     site"
     misc_feature    5621..5623
                     /gene="DICER1"
                     /gene_synonym="Dicer"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (Q6TUI4.3); phosphorylation site"
ORIGIN      

ttgaaacactggatgaatgaaaagccctgctttgcaacccctcagcatggcaggcctgcagctcatgacccctgcttcctcaccaatgggtcctttctttggactgccatggcaacaagaagcaattcatgataacatttatacgccaagaaaatatcaggttgaactgcttgaagcagctctggatcataataccatagtctgtttaaacactggctcagggaagacgtttattgcagtactactcactaaagagctgtcttatcagatcaggggagacttcaacagaaatggcaaaaggacggtgttcttggtcaactctgcaaaccaggttgcccaacaagtgtcagctgtcagaactcactcagatctcaaggtcggggaatactcaaacctagaagtaagtgcatcttggacaaaagagaaatggaaccaagagtttactaagcaccaggttctcattatgacctgctatgtcgccttgaatgttttgaaaaatggttacttatcactgtcagacattaaccttttggtgtttgatgagtgtcatcttgcaatcctagaccacccctaccgagaaattatgaagctttgtgaaaattgtccatcatgtcctcgtattttgggactaactgcttccattttaaatgggaaatgtgatccagaggaattggaagagaagattcagaaactggagaaaattcttaagagtaatgctgaaactgcaactgacttggtggtcttagacagatatacttctcagccatgtgagattgtggtagactgtggaccatttactgacagaagtgggctttatgaaagactgctgatggagttagaagaagcacttaattttatcaatgactgtaacatatctgtacattcaaaagaaagagattctactttaatttctaaacagatactctcagactgccgtgcggtcctggttgtcctgggaccctggtgtgccgataaagtagctggaatgatggtcagagagctgcagaaacacatcaaacatgagcaggaggagctgcaccggaagtttctgttgttcacagacactttcctacggaaaatccacgccctgtgtgaagagcacttctcccctgcctcgcttgacctgaagtttgtcactcctaaagtaataaagctgctcgagatcttacgcaaatacaaaccgtatgagcggcagcagtttgaaagcgtggagtggtataataataggaaccaggataattacgtgtcctggagcgattctgaggatgacgaggaagatgaagagattgaagagaaagaaaagccagagacaaattttccttctccgtttaccaacattttgtgtggaattatttttgtggaaagaagatacacagccgtggtcttaaacagattgataaaggaagctggcaaacaagatccagagctggcttacatcagcagcaattttataactggacatggcatcggaaagaatcagcctcggaacaaacagatggaagcggaattcagaaaacaggaagaggtacttaggaaatttcgagcacatgaaaccaacctgcttattgcaacaagtattgtggaggagggtgttgacataccgaaatgcaacttggtggttcgtttcgatctgcccacagagtatcgatcctacgttcagtccaagggaagagcgagggcacccatctctaattatgtaatgttagcagacacagataaaataaagagttttgaagaagaccttaaaacatacaaagctattgaaaagatcttgcgaaacaaatgttccaagtcggttgataccggggaggccgacacggagcccgtggtggatgacgacgatgttttcccaccgtatgtgctgaggcctgaggacggtccccgcgtcacgatcaacacggccattgggcacgtcaacagatactgtgctagattaccaagtgatccgtttactcatctggctcctaaatgtagaacccgagagttgcctgatggtacattttattcaactctttatctgccaattaactcacctcttcgagcctccattgttggtcccccaatgagctgcattcggttggctgaaagagtcgtggctctcatttgctgtgaaaaactgcacaaaattggcgaactggatgaccatttgatgccagttgggaaggagactgttaaatatgaggaagagctggatttacacgatgaagaagagaccagtgttccaggaagaccaggttccacaaaacgaagacagtgctacccaaaagcaattccagaatgtttgagggagagctaccccaggcctggtcagccctgttacctgtacgtgatcggaatggtcctgacgacacctttacctgatgagctcaacttcagaaggcggaagctctacccccctgaggacaccacaagatgctttggaatactgacagccaagcccatacctcagatcccacactttcctgtgtacacgcgctctggagaggtgaccatatctattgagttgaagaagtccggcttcacgttatctctgcaaatgcttgagttgatcacaaggcttcaccagtatatattctcacatattcttcggcttgaaaaacctgcactagaatttaaacccacagacgcggactcagcctactgcgttctacctcttaatgttgttaatgactccagcactttggacattgactttaaattcatggaagatatcgagaaatctgaagctcgaataggcattcccagcacaaagtattcaaaagaaactccttttgttttcaaattagaagattaccaagatgcagttatcattccaagataccgcaattttgatcagcctcatcgattttatgtcgctgacgtgtacactgatcttacccccctgagtaaatttccttcccctgagtatgaaacgtttgcggagtattataaaacaaagtataaccttgacctgaccaatctcaaccagccgctgctggacgtggaccacacgtcttcacgactcaatcttttgacacctcgtcatttgaatcagaaggggaaagctctccctctgagcagtgctgagaagaggaaagccaagtgggagagcctgcagaataagcagatactggttccagaactctgtgctatacatccaattccagcatcactgtggagaaaagccgtttgtctcccgagcatactttaccgccttcactgccttctgactgcagaggagctcagagcccagacggccagcgacgctggtgtgggagtcaggtcacttcctgtggattttagataccctaacttagacttcgggtggaaaaaatctatcgacagcaaatctttcatctcaattgctaactcctcttcagctgaaaatgagaattactgtaagcacagcacaattgtcgtccctgaaaatgctgcacatcaaggtgctaatagaacctcccctctagaaaatcatgaccaaatgtctgtgaactgcagaacgttgttcagcgagtcacctggtaagctccaaattgaagtttcaacagatctgacagcaattaatggtctttcttacaataaaagtcttgccaatggcagttacgatttagctaacagagacttttgccaaggaaaccatctgaattactacaagcaggaaatacctgtacaaccaactacctcatatcccattcagaatttatacaattacgagaaccagcccaagcccagcgatgaatgtactctactgagtaataaataccttgatggaaatgctaacacatctacctcagatggaagtcccgtgacggctgccgtgcctggtactacagagactggggaggcgcccccggacaggacggcttctgagcagagcccttctcctgggtactcctcgaggacgcttggccccaaccctgggctcattcttcaggctttgaccctttcgaacgctagtgatggatttaacctggagcggctcgagatgcttggcgactccttcctcaagcatgccatcaccaccgtatctctttctgcacttatcctggatgcgcatgagggccgcctttcctacatgagaagcaaaaaggtcagcaactgtaatctgtatcggcttggaaaaaagaaggggctgcccagccgcatggtggtgtccatatttgacccccctgtgaatgggcttcctcctggttacgtagtaaatcaagacaaaagtaacacagagaaatgggaaaaagatgaaatgacaaaagactgcatgttggctaatggcaaactggacgatgacttcgaggaggaggaagaggaggaggaggacctgatgtggagggctcacaaggaggatgcggacgatgaggacgacttcctggaatacgatcaggagcacatcaagttcatagataacatgctgatggggtcaggggctttcgtcaagaaaatctctctttctcctttttctgccaccgattctgcatacgagtggaaaatgcccaaaaagtcctcccttggtagtctgccattctcatccgattttgaggatttcgactacagctcctgggatgcgatgtgctatctggatccgagcaaagccgttgaagaggatgactttgtggtggggttctggaatccatcagaagaaaactgtggtgtggacacagggaagcagtccatctcttacgacctgcacaccgagcagtgcattgccgacaaaagcatagccgactgtgtggaagccctgctgggctgctatttaaccagctgtggcgagagggctgctcagcttttcctctgttcgctggggctgaaggtgctcccggtaattaaaaggactgatcgggaaaaggccatgtgcccgaccagggagaatttcaccagccaacaaaagaacctttcagggagccgtgcggctgcctctggagccggctaccgagcctctgtgttgaaagacttggagtatgggtgtttgaagatccccccgaggtgtatgtttgatcaccccgaggcagaccggacactgcgtcacctgatctcgggttttgaaaatttcgaaaagaaaatcaactacagattcaagaataaggcttaccttctacaggcttttacacacgcctcctaccactacaatacgatcacggattgttaccagcgcttagaattcctgggagatgcaattttggactacctcataactaagcacctttatgaagacccacggcagcactccccaggggtcctgactgacctgcgctctgctctggtcaacaataccatcttcgcgtctttggctgtgaagtatgactaccacaagtactttaaggccgtttctcccgagctcttccatgtcatcgacgatttcgtgcagtttcagctcgagaagaacgaaatgcaaggaatggattctgagcttaggagatccgaggaggatgaagagaaggaagaggatattgaagttccaaaggccatgggggatattttcgagtcactcgcgggcgccatttacatggacagcgggatgtcgctggagacggtctggcaggtgtactatcccatgatgcggccgctgatcgaaaaattttctgcaaatgtgccccgttcccctgtgcgagaattgcttgaaatggaaccagaaattaccaaattcagcccagctgagagaacctacgatggcaaggtcagagtcaccgtggaagtggtcgggaaggggaagttcaaaggcgttggccgaagttacaggattgccaagtctgcagcagcaagaagagccctgcgaagcctcaaagctaatcaacctcaggttcccaatagctgaaaacccctttttaaattaaaaaaaaaaaaaaaaaaaaaaa

//