LOCUS       NM_015776               1505 bp    mRNA    linear   ROD 28-NOV-2023
DEFINITION  Mus musculus microfibrillar associated protein 5 (Mfap5),
            transcript variant 1, mRNA.
ACCESSION   NM_015776
VERSION     NM_015776.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Jacob T, Annusver K, Czarnewski P, Dalessandri T, Kalk C, Levra
            Levron C, Campama Sanz N, Kastriti ME, Mikkola ML, Rendl M,
            Lichtenberger BM, Donati G, Bjorklund AK and Kasper M.
  TITLE     Molecular and spatial landmarks of early mouse skin development
  JOURNAL   Dev Cell 58 (20), 2140-2162 (2023)
   PUBMED   37591247
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Hofling C, Rossner S, Flachmeyer B, Krueger M, Hartig W and
            Michalski D.
  TITLE     Tricellulin, alpha-Catenin and Microfibrillar-Associated Protein 5
            Exhibit Concomitantly Altered Immunosignals along with Vascular,
            Extracellular and Cytoskeletal Elements after Experimental Focal
            Cerebral Ischemia
  JOURNAL   Int J Mol Sci 24 (15), 11893 (2023)
   PUBMED   37569268
  REMARK    GeneRIF: Tricellulin, alpha-Catenin and Microfibrillar-Associated
            Protein 5 Exhibit Concomitantly Altered Immunosignals along with
            Vascular, Extracellular and Cytoskeletal Elements after
            Experimental Focal Cerebral Ischemia.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1505)
  AUTHORS   Chen Z, Zhao H, Meng L, Yu S, Liu Z and Xue J.
  TITLE     Microfibril-Associated Glycoprotein-2 Promoted Fracture Healing via
            Integrin alphavbeta3/PTK2/AKT Signaling
  JOURNAL   Lab Invest 103 (7), 100121 (2023)
   PUBMED   36934797
  REMARK    GeneRIF: Microfibril-Associated Glycoprotein-2 Promoted Fracture
            Healing via Integrin alphavbeta3/PTK2/AKT Signaling.
REFERENCE   4  (bases 1 to 1505)
  AUTHORS   Han C, Leonardo TR, Romana-Souza B, Shi J, Keiser S, Yuan H,
            Altakriti M, Ranzer MJ, Ferri-Borgogno S, Mok SC, Koh TJ, Hong SJ,
            Chen L and DiPietro LA.
  TITLE     Microfibril-associated protein 5 and the regulation of skin scar
            formation
  JOURNAL   Sci Rep 13 (1), 8728 (2023)
   PUBMED   37253753
  REMARK    GeneRIF: Microfibril-associated protein 5 and the regulation of
            skin scar formation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1505)
  AUTHORS   Wu L, Zhou F, Xin W, Li L, Liu L, Yin X, Xu X, Wang Y and Hua Z.
  TITLE     MAGP2 induces tumor progression by enhancing uPAR-mediated cell
            proliferation
  JOURNAL   Cell Signal 91, 110214 (2022)
   PUBMED   34915136
  REMARK    GeneRIF: MAGP2 induces tumor progression by enhancing uPAR-mediated
            cell proliferation.
REFERENCE   6  (bases 1 to 1505)
  AUTHORS   Nehring LC, Miyamoto A, Hein PW, Weinmaster G and Shipley JM.
  TITLE     The extracellular matrix protein MAGP-2 interacts with Jagged1 and
            induces its shedding from the cell surface
  JOURNAL   J Biol Chem 280 (21), 20349-20355 (2005)
   PUBMED   15788413
REFERENCE   7  (bases 1 to 1505)
  AUTHORS   Lemaire R, Farina G, Kissin E, Shipley JM, Bona C, Korn JH and
            Lafyatis R.
  TITLE     Mutant fibrillin 1 from tight skin mice increases extracellular
            matrix incorporation of microfibril-associated glycoprotein 2 and
            type I collagen
  JOURNAL   Arthritis Rheum 50 (3), 915-926 (2004)
   PUBMED   15022335
  REMARK    GeneRIF: Tight skin fibrillin 1 altered extracellular matrix
            organization and caused fibrosis by affecting deposition of MAGP-2
            or other fibrillin-1-associated proteins.
REFERENCE   8  (bases 1 to 1505)
  AUTHORS   Penner AS, Rock MJ, Kielty CM and Shipley JM.
  TITLE     Microfibril-associated glycoprotein-2 interacts with fibrillin-1
            and fibrillin-2 suggesting a role for MAGP-2 in elastic fiber
            assembly
  JOURNAL   J Biol Chem 277 (38), 35044-35049 (2002)
   PUBMED   12122015
  REMARK    GeneRIF: interaction with fibrillin-1 and fibrillin-2 suggesting
            role in elastic fiber assembly
REFERENCE   9  (bases 1 to 1505)
  AUTHORS   Shipley JM, Mecham RP, Maus E, Bonadio J, Rosenbloom J, McCarthy
            RT, Baumann ML, Frankfater C, Segade F and Shapiro SD.
  TITLE     Developmental expression of latent transforming growth factor beta
            binding protein 2 and its requirement early in mouse development
  JOURNAL   Mol Cell Biol 20 (13), 4879-4887 (2000)
   PUBMED   10848613
REFERENCE   10 (bases 1 to 1505)
  AUTHORS   Frankfater C, Maus E, Gaal K, Segade F, Copeland NG, Gilbert DJ,
            Jenkins NA and Shipley JM.
  TITLE     Organization of the mouse microfibril-associated glycoprotein-2
            (MAGP-2) gene
  JOURNAL   Mamm Genome 11 (3), 191-195 (2000)
   PUBMED   10723723
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155946.7.
            
            On Mar 19, 2018 this sequence version replaced NM_015776.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF180805.1, AK003479.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-149               AC155946.7         121370-121518
            150-209             AC155946.7         122127-122186
            210-245             AC155946.7         123134-123169
            246-278             AC155946.7         128365-128397
            279-311             AC155946.7         128682-128714
            312-347             AC155946.7         129679-129714
            348-371             AC155946.7         132273-132296
            372-459             AC155946.7         133737-133824
            460-533             AC155946.7         134573-134646
            534-1505            AC155946.7         136105-137076
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 57.61 cM"
     gene            1..1505
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /note="microfibrillar associated protein 5"
                     /db_xref="GeneID:50530"
                     /db_xref="MGI:MGI:1354387"
     exon            1..149
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    86..88
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /note="upstream in-frame stop codon"
     exon            150..209
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..646
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; microfibril-associated glycoprotein 2"
                     /codon_start=1
                     /product="microfibrillar-associated protein 5 isoform 1
                     precursor"
                     /protein_id="NP_056591.1"
                     /db_xref="CCDS:CCDS20496.1"
                     /db_xref="GeneID:50530"
                     /db_xref="MGI:MGI:1354387"
                     /translation="

MLFLGQKALLLVLAISIPSDWLPLGVSGQRGDDVPETFTDDPNLVNDPSTDDTALADITPSTDDLAGDKNATAECRDEKFACTRLYSVHRPVRQCVHQSCFTSLRRMYIINNEICSRLVCKEHEAMKDELCRQMAGLPPRRLRRSNYFRLPPCENMNLQRPDGL"

     sig_peptide     152..235
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
     misc_feature    155..553
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /note="Microfibril-associated glycoprotein (MAGP); Region:
                     MAGP; pfam05507"
                     /db_xref="CDD:428500"
     mat_peptide     236..643
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /product="Microfibrillar-associated protein 5.
                     /id=PRO_0000018686"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZJ6.1)"
     misc_feature    239..247
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZJ6.1);
                     Region: Cell attachment site. /evidence=ECO:0000255"
     misc_feature    359..361
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9QZJ6.1); glycosylation site"
     exon            210..245
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="alignment:Splign:2.1.0"
     exon            246..278
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="alignment:Splign:2.1.0"
     exon            279..311
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="alignment:Splign:2.1.0"
     exon            312..347
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="alignment:Splign:2.1.0"
     exon            348..371
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="alignment:Splign:2.1.0"
     exon            372..459
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="alignment:Splign:2.1.0"
     exon            460..533
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="alignment:Splign:2.1.0"
     exon            534..1505
                     /gene="Mfap5"
                     /gene_synonym="MAGP-2; MFAP-5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ttagaataggccaaatgtgaagtctttccaagggtgtggctgtgttcatcttattctccagcccaagtagaacagcagagaagcgtgagaaggacggacacactcagcagccagaggccaccggcagacagatcgcagctctgtagacaatatgctgttcttggggcagaaggccttgctgcttgtcttggcaatcagcatcccctctgactggctacccctaggggtcagtggtcaacgcggagatgatgtgcctgagacattcacagacgaccctaatctggtgaacgatccctctacagacgatacagctctggctgatatcacaccttccacagatgacctagctggtgacaaaaatgctactgcagagtgccgggatgagaagtttgcttgtacaagactgtactctgtccatcggccagtcagacagtgtgtgcaccagtcctgcttcaccagtttacggcgcatgtacatcatcaataatgagatctgttcccgactcgtctgtaaagaacatgaagctatgaaagatgagctttgccggcagatggcaggcctgcctccaaggcgacttcggcgctccaactacttccgacttcctccctgtgaaaatatgaatttgcagagacccgatggtctgtgatcaccaaggaagaaagaagaaaatgtggatgaaggaatcgaaaattctttctcctccaacccctgccatctgtcccgtagacatgtatttttaaactaagccctttgcaatgccccggcttcctaccctactctaattttcactggtgctggtaacgtttgtctcattttgcggtactgacaatacattgtctatattgtggcacgggtttgctgatttctggtgttgtagacaagatggataaaggatggccacttgacttagatacattcagctacattcagctcctgcagtagaaaggggctggggtgcgtggctcagttggcagaaaacttacctaacctttaggttaatattttttttaaatggtgctatgaattgatctcagagcctcgtatataatagtcaatgctctatcactaagctatacccctatctcctaagtagataattttaattattaaatatgccagagacaggagcttgccagaggctcgagccacagagcttccttctagaagtttcctaggttaactcctcattgtttacatatcctctgtactcactttcaccatgcttcgtggcttgtcctaaattttgcccagcaagtcttcatgatgggaaggttgtgggtgaggcaagagagatggctcaatggttaagaacactggatgttcttccagaggacctgggttcagttcccagccagcatccacatggtggctcacaatcacccataagtctagttccagaggacctgagatattttctggcatccatgggcactgcatgcatatgtcacacagacatacatgtggaaaacacacataaaaataaatcactttttaaaaggcaaaaa

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