LOCUS       NM_001407215            4279 bp    mRNA    linear   ROD 23-NOV-2023
DEFINITION  Mus musculus protein-L-isoaspartate (D-aspartate)
            O-methyltransferase 1 (Pcmt1), transcript variant 31, mRNA.
ACCESSION   NM_001407215
VERSION     NM_001407215.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Zhang C, Guo ZF, Liu W, Kazama K, Hu L, Sun X, Wang L, Lee H, Lu L,
            Yang XF, Summer R and Sun J.
  TITLE     PIMT is a novel and potent suppressor of endothelial activation
  JOURNAL   Elife 12, e85754 (2023)
   PUBMED   37070640
  REMARK    GeneRIF: PIMT is a novel and potent suppressor of endothelial
            activation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Qin Z, Dimitrijevic A and Aswad DW.
  TITLE     Accelerated protein damage in brains of PIMT+/- mice; a possible
            model for the variability of cognitive decline in human aging
  JOURNAL   Neurobiol Aging 36 (2), 1029-1036 (2015)
   PUBMED   25465735
  REMARK    GeneRIF: PIMT levels may significantly influence the course of
            age-related central nervous system dysfunction.
REFERENCE   3  (bases 1 to 4279)
  AUTHORS   Dimitrijevic A, Qin Z and Aswad DW.
  TITLE     Isoaspartyl formation in creatine kinase B is associated with loss
            of enzymatic activity; implications for the linkage of isoaspartate
            accumulation and neurological dysfunction in the PIMT knockout
            mouse
  JOURNAL   PLoS One 9 (6), e100622 (2014)
   PUBMED   24955845
  REMARK    GeneRIF: Loss of normal CKB structure and function contributes to
            the mechanisms by which isoaspartate accumulation leads to central
            nervous system dysfunction in the PIMT-Knockout mouse.
            Erratum:[PLoS One. 2014;9(12):e116556]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4279)
  AUTHORS   Qin Z, Yang J, Klassen HJ and Aswad DW.
  TITLE     Isoaspartyl protein damage and repair in mouse retina
  JOURNAL   Invest Ophthalmol Vis Sci 55 (3), 1572-1579 (2014)
   PUBMED   24550364
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4279)
  AUTHORS   Kapadia B, Viswakarma N, Parsa KV, Kain V, Behera S, Suraj SK, Babu
            PP, Kar A, Panda S, Zhu YJ, Jia Y, Thimmapaya B, Reddy JK and Misra
            P.
  TITLE     ERK2-mediated phosphorylation of transcriptional coactivator
            binding protein PIMT/NCoA6IP at Ser298 augments hepatic
            gluconeogenesis
  JOURNAL   PLoS One 8 (12), e83787 (2013)
   PUBMED   24358311
  REMARK    GeneRIF: ERK2-mediated phosphorylation of transcriptional
            coactivator binding protein PIMT/NCoA6IP at Ser298 augments hepatic
            gluconeogenesis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4279)
  AUTHORS   MacLaren DC and Clarke S.
  TITLE     Rapid mapping of genomic P1 clones: the mouse
            L-isoaspartyl/D-aspartyl methyltransferase gene
  JOURNAL   Genomics 35 (2), 299-307 (1996)
   PUBMED   8661142
REFERENCE   7  (bases 1 to 4279)
  AUTHORS   Galus A, Lagos A, Romanik EA and O'Connor CM.
  TITLE     Structural analysis of transcripts for the protein L-isoaspartyl
            methyltransferase reveals multiple transcription initiation sites
            and a distinct pattern of expression in mouse testis:
            identification of a 5'-flanking sequence with promoter activity
  JOURNAL   Arch Biochem Biophys 312 (2), 524-533 (1994)
   PUBMED   8037467
REFERENCE   8  (bases 1 to 4279)
  AUTHORS   Warden CH, Mehrabian M, He KY, Yoon MY, Diep A, Xia YR, Wen PZ,
            Svenson KL, Sparkes RS and Lusis AJ.
  TITLE     Linkage mapping of 40 randomly isolated liver cDNA clones in the
            mouse
  JOURNAL   Genomics 18 (2), 295-307 (1993)
   PUBMED   8288233
REFERENCE   9  (bases 1 to 4279)
  AUTHORS   MacLaren DC, O'Connor CM, Xia YR, Mehrabian M, Klisak I, Sparkes
            RS, Clarke S and Lusis AJ.
  TITLE     The L-isoaspartyl/D-aspartyl protein methyltransferase gene (PCMT1)
            maps to human chromosome 6q22.3-6q24 and the syntenic region of
            mouse chromosome 10
  JOURNAL   Genomics 14 (4), 852-856 (1992)
   PUBMED   1478665
REFERENCE   10 (bases 1 to 4279)
  AUTHORS   Romanik EA, Ladino CA, Killoy LC, D'Ardenne SC and O'Connor CM.
  TITLE     Genomic organization and tissue expression of the murine gene
            encoding the protein beta-aspartate methyltransferase
  JOURNAL   Gene 118 (2), 217-222 (1992)
   PUBMED   1511895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC156391.6.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14995048.2119050.1,
                                           SRR17253011.1196778.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-522               AC156391.6         112794-113315
            523-627             AC156391.6         127235-127339
            628-659             AC156391.6         128769-128800
            660-764             AC156391.6         131961-132065
            765-885             AC156391.6         135575-135695
            886-971             AC156391.6         136260-136345
            972-1147            AC156391.6         138165-138340
            1148-4279           AC156391.6         145414-148545
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 2.26 cM"
     gene            1..4279
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="protein-L-isoaspartate (D-aspartate)
                     O-methyltransferase 1"
                     /db_xref="GeneID:18537"
                     /db_xref="MGI:MGI:97502"
     exon            1..522
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    141..143
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="upstream in-frame stop codon"
     misc_feature    294..296
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="upstream_AUG_codon; putative N-terminal extension:
                     MPGARIGGSGSDGSNSGRSSGDTSGAVTVWEVVSLLGKLLGTVAALKVVLYLLRVCFA
                     "
     CDS             468..1160
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /EC_number="2.1.1.77"
                     /note="isoform 11 is encoded by transcript variant 31;
                     protein-L-isoaspartate(D-aspartate) O-methyltransferase;
                     protein-beta-aspartate methyltransferase; L-isoaspartyl
                     protein carboxyl methyltransferase; protein
                     L-isoaspartyl/D-aspartyl methyltransferase"
                     /codon_start=1
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase isoform 11"
                     /protein_id="NP_001394144.1"
                     /db_xref="GeneID:18537"
                     /db_xref="MGI:MGI:97502"
                     /translation="

MAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKSNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGNSGKVIGIDHIKELVDDSITNVKKDDPMLLSSGRVRLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQWSRWKDEL"

     mat_peptide     471..1148
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /product="Protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase. /id=PRO_0000111876"
                     /note="propagated from UniProtKB/Swiss-Prot (P23506.3)"
     misc_feature    471..473
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="N-acetylalanine.
                     /evidence=ECO:0000250|UniProtKB:P22061; propagated from
                     UniProtKB/Swiss-Prot (P23506.3); acetylation site"
     misc_feature    489..1115
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="Protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase (PCMT); Region: PCMT; pfam01135"
                     /db_xref="CDD:395902"
     exon            523..627
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            628..659
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            660..764
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            765..885
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            886..971
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1147
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..4279
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gcggagcccaagaaggcggcacacgtcggacccgccatcccgcatcagccagagccgcggctaggaaggagcctcatcacgcagaacaccagctcgccggccgcaggacggaggcaggtgcaggcctcaggaaggggaactgagcagcgttcgtttgtgcgacctcgctgcggccaccgagcactggttatcgcccccgctcgctactgggctccaggactcccccgccgcgggccccgcctggccgcaccccggactgcgcggcgtcacagagcgcatgcgtgccgcgggggatgccgggagcgcgcattggcggcagtggcagcgacggcagtaacagcggccgctcgagcggggacaccagcggggcggtgaccgtgtgggaggtggtctcactcttggggaaactgctagggaccgtcgccgcgctgaaggtggttctgtacctgctccgggtgtgcttcgcgatggcctggaaatccggcggcgccagccactcggagctaatccacaacctccgcaagaatggaatcatcaagacagataaagtatttgaagtgatgctggctacagaccgttcccactatgcaaaatctaacccttacatggattctccacaatcaataggtttccaggcaacaatcagtgcccctcatatgcacgcatatgcactagaacttctgtttgaccagctgcatgaaggtgctaaagccctggacgtgggctctggaagtggaatcctcaccgcctgttttgcacggatggttggaaatagtggaaaagtcattggaattgatcacattaaagaactagtagatgactcaataactaatgtcaaaaaggatgacccaatgctcctgtcctcggggcgagtgcggctggtggtgggtgatggaagaatgggatacgctgaagaagccccttacgacgctattcatgtgggagctgcagccccagttgtgccccaggcattaatagaccagttaaagcctggtggaagattgatattgccagtcggtcctgcaggaggaaaccaaatgttggagcagtatgacaagctacaagatggcagtgtcaaaatgaaacctctcatgggggtgatatacgtgcctttaacagataaagaaaagcagtggtccaggtggaaggatgaactgtgaaagcaacgtgagcttgaccagtctataaaccgcagtgggccgctcctctcggtccttacagcatttgagaaccaacagcatcacagcttgttttggactttgttacactcttactttcagcatgaaaacgtgtggttttgtaggatttcctaattccaaacggcgcggagccaaactgatagaggaaggctgcagagcgttaactgcgtgtgttgttttaacatgcccacatttcactttaaaaaaatattaaaagaaagagttctccaactggaaaaattagttttgaagagtggctttgcttttcttgggcacttaattctgttccaatattaattcatcagatttattttgtgcatcagataacaccacattcacaagtttagcttctttggtagccaatgtctgttagatgctactaagaaaacagagatgagctttcttcttaaaagcctttgatgtggcgtcataggatagcatgtcgtagatacaatcagctgctgttaccttaaaactaggtgtttgtaaatactgaaacttgagacgacgatgatggcaggtggtgtcccaaaaacactcagctgttccgattggaaattttgatgtaaatttcctctctctaaaaatgctaggaggcttgtagctcaatatcatttatttgtttctagtttgctgctggtaatgtatctgcatctaacattctgtgtaagcggtcgtgtctttgcacagtttatgcagcgctgctttgccaaataaaattaaaagcaaaagaagcattggtggcctttgaatctagaaggacacaggtctgctaggtccagatgctgcccacagtcctggggaagcgtcgtagtcttatgatattctaggaggtagatatgcatagcattcgtccactctatgtggctgcatttcttcaggatggagactctgtgttcattgtttctctctgccttcctagcccaaggcttgctttttaatagctctgtagtaaatgctcaatgagtgactgggtgaatgtagtatgaggaagaatgtggttgagagtaaacatactgaaatccatgtctcatctataaatagattcattaatttaataactgtaaaatgtctgtgaaggcaatggcactttgaagatcttatttaaatatggttttatattttctaataagactttagttttttgtgaagatcctagaacttgagagaagattagaagctcccggtaaatggggctaagcaaagcgaagccttccctctatgtgtaagggcacatgattctgacaagaagactcttagtgtctgagaagcccatccccactcttcacatcttaaattactgatttaaaagtcatgcagacatcacctgacaagtgtctcttcttggcttaggtctcagtcccaggaaactagcattactgtggtcccgcagacttgaaaaccttaaccagctttggtttgctgctggttgttgctttggaatcctgtcagtgaccccctggagtccaggatgcatggtcatttgaaatatgtgtaaagtcaaatgttcactcaggctttaaagcaaataaattcttaaacttacccaccagtctggatccttggtagtcattgcacattacagcacgcaaatctctgtgctgggcttcaggacggaagaggtgcttccaggtcagtttgccgtccagtggcggggacagtttataaataggcaccatacacttcacccagtttgtacagaaactctgtgcccaagccttgctcttagacttgacacttttaggaggtgaaactttaggatgttgaatgaacagccagtttgaacagcagcactaagtgggtagagatagaagaatggtgttaatgaggtctaagccctttaaaagagttgaatttatatatgtgtttaagacaggtaagaaatgcctgattttcagtgtgcataatttccagagcattttattttcctcatagaaagtacatctgaatacacataatgtaaagggcaatgaagatacttcgagtcattataggtagtgtgatcttgtgaataaactcttcctataaatttgctcttcctagaagctaaatctagttgtcagacttgggttgcagcaataagcttatgtcagggttgagtttctcttcagggctgcttgctctttcccagccaggaacaggacaaatcggcacccagggaaataactactactggccacaccaatacttaggacttcatcccccttgcagctgccatgttttattgtttgtttgtttatttgtggcaggtgggggtggatagcaagatggctcggggtaaagaggcttgctgccaaacctgaagacctgagtttcacccctcccccctcccagacccacagagtagaaggacagctgacacctgcagattgttctctgaccttcaggcatggtagcacttgcctgaaatcccagcacttggaagcaggaagggtcagaagtcatcctaggttacacaagttcaaggccagcccgaatttcatgagagcctgccttaaaggaaagaaaaaaccaaaaagtttctacagtgcatctcttcactaaagcatcgtaagcattgagtggattctaaaggagccatttttaaagtaataactttgtaaactcttcaagctgttcttgaaggaattcctcagagtgtttaaggccttttatctctctagagttttctaatttctggtagtttagtgtggcatcaaagaaagtcatgacagtgttgtcacccagcactgatagacaaatagtaagcatgagtcatcttataaattgttgtagaactcaaccggctgataacttattttaggtaagcacttaaactcttgtagcatacagatcatttcttatcgaatgctgtgttcctgatgccctaatatcttaatgtattctgtagtggagatgggccctgactggactttggttgcatctctgagcttccaccatctttcagaaagatggtagtattttgcccttagcattgttaaaggttctcactcagtaaggggacccacttgaaaagggtactggcccaaggaagcctgggactgtgcactggaatataataaagtggaaatgaagtctaa

//