LOCUS       NM_001244269            5818 bp    mRNA    linear   ROD 24-DEC-2022
DEFINITION  Cricetulus griseus dicer 1, ribonuclease III (Dicer1), mRNA.
ACCESSION   NM_001244269
VERSION     NM_001244269.1
KEYWORDS    RefSeq.
SOURCE      Cricetulus griseus (Chinese hamster)
  ORGANISM  Cricetulus griseus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Cricetidae; Cricetinae; Cricetulus.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Becker J, Hackl M, Rupp O, Jakobi T, Schneider J, Szczepanowski R,
            Bekel T, Borth N, Goesmann A, Grillari J, Kaltschmidt C, Noll T,
            Puhler A, Tauch A and Brinkrolf K.
  TITLE     Unraveling the Chinese hamster ovary cell line transcriptome by
            next-generation sequencing
  JOURNAL   J Biotechnol 156 (3), 227-235 (2011)
   PUBMED   21945585
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from EF031271.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EF031271.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMN04275174, SAMN04275175
                                           [ECO:0000350]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Cricetulus griseus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10029"
                     /chromosome="5"
                     /map="5"
     gene            1..5818
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="dicer 1, ribonuclease III"
                     /db_xref="GeneID:100689239"
     exon            1..185
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    39..41
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="upstream in-frame stop codon"
     CDS             42..5795
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /EC_number="3.1.26.3"
                     /note="Dicer1, Dcr-1 homolog; dicer 1, ribonuclease type
                     III"
                     /codon_start=1
                     /product="endoribonuclease Dicer"
                     /protein_id="NP_001231198.1"
                     /db_xref="GeneID:100689239"
                     /translation="

MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPRAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWSQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYASQPCEIVVDCGPFTDRSGLYERLLMELEEAINFINDCNVSVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEHFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIQSFEEDLKTYKAIEKILRNKCSKSVDGAEADVDAVVDDDDVFPPYVLRPDDGGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTQELPDGTFYSTLYLPINSPLRASIVGPPMGCVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSSTLDIDFTFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISTCNSSLAENDNYCKHSTIIVPENAAHQGATRTPLENHDQMSVNCRRLPAESSGKLQIEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYHYENQPKPSNECTLLSNKYLDGNANTSTSDGSPAVSTMPAVMSAGRALKDRMDSEQSPSAGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNAEKWEKDEMTKDCLLANGKLGKDCEEEEALTWRAPKEEAEYEDDFLEYDQEHIQFIDSMLMGSGAFVKKISLSPFSTSDSAYEWKMPKKSSLGSMPFSSDLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALYPAQENFSSQQKSLSGSCAATAASPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS"

     misc_feature    165..758
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="DEXH-box helicase domain of endoribonuclease Dicer;
                     Region: DEXHc_dicer; cd18034"
                     /db_xref="CDD:350792"
     misc_feature    order(165..176,183..185,234..257,378..380,567..569,
                     660..662)
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:350792"
     misc_feature    order(342..347,414..419,492..494,498..503,510..512,
                     585..593)
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="nucleic acid binding site [nucleotide binding];
                     other site"
                     /db_xref="CDD:350792"
     misc_feature    564..575
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="propagated from UniProtKB/Swiss-Prot (A0MQH0.3);
                     Region: DECH box"
     misc_feature    807..1826
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="propagated from UniProtKB/Swiss-Prot (A0MQH0.3);
                     Region: Required for interaction with PRKRA and TARBP2.
                     /evidence=ECO:0000250"
     misc_feature    852..1136
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Partner-binding domain of the endoribonuclease
                     Dicer; Region: Dicer_PBD; cd15903"
                     /db_xref="CDD:277191"
     misc_feature    order(867..872,879..881,885..890,1065..1070,1077..1082,
                     1089..1091,1098..1103,1110..1112)
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Trbp binding interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:277191"
     misc_feature    1155..1733
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="C-terminal helicase domain of the endoribonuclease
                     Dicer; Region: SF2_C_dicer; cd18802"
                     /db_xref="CDD:350189"
     misc_feature    1266..1340
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="propagated from UniProtKB/Swiss-Prot (A0MQH0.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1278..1280
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (A0MQH0.3); phosphorylation site"
     misc_feature    1284..1286
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (A0MQH0.3); phosphorylation site"
     misc_feature    order(1386..1394,1602..1604,1659..1661,1665..1667)
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350189"
     misc_feature    order(1614..1616,1620..1622,1695..1697,1701..1703)
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:350189"
     misc_feature    1929..2195
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Dicer dimerization domain; Region: Dicer_dimer;
                     pfam03368"
                     /db_xref="CDD:427264"
     misc_feature    2697..3065
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="PAZ domain, dicer_like subfamily. Dicer is an RNAse
                     involved in cleaving dsRNA in the RNA interference
                     pathway. It generates dsRNAs which are approximately 20 bp
                     long (siRNAs), which in turn target hydrolysis of
                     homologous RNAs. PAZ domains are named...; Region:
                     PAZ_dicer_like; cd02843"
                     /db_xref="CDD:239209"
     misc_feature    order(2886..2888,2925..2927,2943..2945,2955..2957,
                     3009..3011,3033..3035,3039..3041)
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239209"
     misc_feature    3087..3089
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (A0MQH0.3); phosphorylation site"
     misc_feature    3519..3521
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (A0MQH0.3); phosphorylation site"
     misc_feature    3927..>4202
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Ribonuclease III family; Region: RIBOc; smart00535"
                     /db_xref="CDD:197778"
     misc_feature    order(3978..3983,3990..3995,4002..4004,4011..4016,
                     4023..4028,4032..4040,4068..4070)
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="dimerization interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:238333"
     misc_feature    4407..4409
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (A0MQH0.3); phosphorylation site"
     misc_feature    4431..4433
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8R418; propagated from
                     UniProtKB/Swiss-Prot (A0MQH0.3); phosphorylation site"
     misc_feature    4437..4439
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (A0MQH0.3); phosphorylation site"
     misc_feature    5022..5780
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="dsRNA-specific ribonuclease [Transcription];
                     Region: Rnc; COG0571"
                     /db_xref="CDD:223644"
     misc_feature    5442..5444
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Important for activity. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (A0MQH0.3); other
                     site"
     misc_feature    5628..5630
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UPY3; propagated from
                     UniProtKB/Swiss-Prot (A0MQH0.3); phosphorylation site"
     exon            186..348
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            349..479
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            480..614
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            615..775
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            776..944
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1417
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1550
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1793
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1948
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2081
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2157
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2297
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            2298..2477
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            2478..2691
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            2692..2845
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..3028
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            3029..3134
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            3135..3310
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            3311..4091
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            4092..4247
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            4248..5121
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            5122..5390
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            5391..5553
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            5554..5629
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
     exon            5630..5818
                     /gene="Dicer1"
                     /gene_synonym="DICER"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gctgtgctagaacaaaatgcaatgaaagaaacactggatgaatgaaaagccctgctttgcagcccctcagcatggcaggcctgcagctcatgacccctgcttcctcaccaatgggtcctttctttggactgccatggcaacaagaagcaattcatgataacatttatacgccaagaaaataccaggttgagctgctggaagcggctctggaccataacaccattgtctgcttgaacaccggctcagggaagacgttcatcgcggtcctgctcaccaaagagctggcccatcagatcaggggcgacctcaacccacgtgcaaaaaggaccgtgttcctcgtcaactctgcaaaccaggttgctcaacaagtttcagctgtcagaactcactcagacctcaaggttggggagtactcaaacctagaagtaaatgcatcttggacaaaagagagatggagccaagagtttactaagcaccaggttctcattatgacttgctatgtcgccttgaatgttttgaaaaatggttacttatcactgtcagacattaaccttttggtgtttgatgagtgtcatcttgcaattctagaccacccttaccgagaaattatgaagctctgtgaaagttgtccatcatgccctcgaattttgggactaactgcttccattttaaatgggaaatgtgatccagaggaattggaagaaaagattcagaaactggagaagattcttaagagtaatgctgagactgccactgacctggtggtcttagacagatacgcttcccagccatgtgagattgtggtagattgtggaccatttactgacagaagtgggctttatgaaaggctgctgatggaattagaagaagcaattaattttatcaatgactgcaatgtatctgtacattcaaaggaaagggattctactctgatttccaaacaaatcctctcagactgccgtgctgtgttggttgttctgggaccgtggtgtgcggataaagtagctgggatgatggtaagagaactgcagaagtacatcaaacatgagcaagaggagctgcacaggaaattcttattgtttacagacactttgctgaggaaaatacatgcactgtgtgaagagcacttctcacctgcctcacttgacctgaagtatgtaactcctaaagtaatgaaactgctcgagatcctgcgcaaatacaagccctatgagcgacagcagtttgagagtgttgagtggtacaataataggaatcaagataattatgtgtcctggagtgattctgaggatgatgatgacgatgaagaaattgaagaaaaagaaaagccagagacaaattttccttctccatttaccaatatcttatgtggaattatttttgtggaaagaagatacacagcagttgtcctaaacagattgataaaggaagctggcaagcaagatccagagctggcttatatcagtagcaactttataactggacatggcattgggaagaaccagcctcgcagcaaacagatggaagcagagttcagaaaacaggaggaggtacttaggaaattccgagcacacgagactaacctgctcattgcaacaagtgttgtagaagagggtgtggatattcccaagtgcaacttggtggttcgttttgatttgcccacggagtatcgatcatatgttcagtccaaaggacgagcccgagccccgatctctaattatgtgatgttagccgatacagacaagatacaaagctttgaagaagaccttaaaacctacaaagctatagaaaagatcttaagaaacaagtgttccaagtcagtggatggtgctgaggctgacgtcgatgccgttgtagacgatgatgatgttttcccaccatatgtattgaggcctgacgatgggggtccacgggtcaccatcaacactgccattggacatgttaacagatactgtgctcgattaccaagtgatccgtttactcatctagctcccaaatgtagaacccaagagttgcctgatggtacattttattcaactctttatcttccgattaactcacctctgcgagcctccattgttggtccaccaatgggctgtgtccggttggctgaaagagtggtagctctcatttgctgtgaaaaactgcacaaaattggtgaactggatgaacatttgatgccagttggaaaagagactgttaaatatgaagaagagcttgacttacatgatgaagaggaaaccagtgttccaggaagaccagggtccacaaagcgaaggcagtgctaccccaaagcaatcccagagtgcttgagggagagttaccctaaacccgatcagccttgttacctgtatgtgatagggatggtcttaactacccccctccctgatgagctcaacttcaggcgacggaaactctaccctcctgaggacaccacaagatgctttgggatcctgacagccaaacccatacctcagattcctcactttcctgtgtacacacgctctggagaagtcaccatatccattgagctgaagaagtctggctttacactgtctcaacaaatgcttgagctgattacaagactccaccagtatatattctcacacattcttcggcttgagaagcccgccctagagtttaaaccaacaggcgctgagtcagcatactgtgttctacctcttaatgtcgttaatgactctagcactctggacattgactttacattcatggaggatattgagaagtctgaagctcgaataggcattcccagcactaagtattcaaaagaaacaccgtttgtttttaagttagaagattaccaagatgcagtgatcattcccagataccgaaactttgatcagcctcatcgattttatgtagctgatgtgtatactgatcttaccccactcagtaaatttccttcccccgagtatgaaacttttgcagaatattataaaacaaagtataaccttgacctgaccaatctcaaccagccactgctggatgttgaccacacctcttccagacttaaccttttgacacctcggcatttgaatcagaaggggaaagcacttcctctaagcagtgctgaaaagaggaaagccaaatgggagagtctgcagaacaaacagatactggttccggaactctgtgctatacatccaatcccagcatcactgtggagaaaagcagtttgtctccccagcatactttatcgccttcactgccttttgactgcagaggagctaagagctcagacagccagcgatgctggtgtgggagtcagatcacttcctgcggattttagataccctaacttagacttcgggtggaaaaaatctattgatagcaagtctttcatctcaacttgtaactcctcgttggctgaaaatgataattactgtaagcacagcacaattatagtccctgaaaatgctgcacatcaaggtgctactagaacccccttagaaaaccatgaccaaatgtctgtgaactgcagaaggctacccgctgagtcatctggtaagctccaaattgaagtttcaacggaccttacagcaattaatggtctttcttacaacaaaaatcttgccaatggcagttatgatttagttaacagagacttttgccaaggaaatcagctgaattacttcaagcaggaaatacctgtacaaccaactacctcatatcccattcagaatttataccattatgagaaccagcccaagcccagcaatgaatgtactctactgagtaataaataccttgatggaaacgctaacacatctacctcagatggaagccccgcggtgtccacgatgcctgccgtgatgagtgctgggagagcactcaaggacaggatggattctgagcagagcccttctgctgggtactcctcaaggactcttggccccaatcctggactgattcttcaggctttgacactgtcaaatgctagtgatgggtttaacctggagcggcttgagatgcttggcgactcctttttaaaacatgctatcaccacatatctcttttgcacttaccctgatgctcacgagggccgcctttcatatatgagaagcaaaaaggtcagcaactgtaacctgtatcgccttggcaagaagaagggactgcccagccgcatggtagtgtcaatatttgatcctcctgtgaattggcttcctcctggttatgtggtcaatcaagacaaaagtaatgcagagaaatgggaaaaggatgaaatgacaaaagactgcttgctggctaatggcaaactaggtaaggactgtgaggaggaggaggccctgacgtggagggctcccaaagaggaggctgagtatgaagacgacttcctggaatatgatcaggagcacatccagtttatagacagcatgttaatggggtcaggagcctttgtgaagaaaatttctctttctcctttttcaacctccgattctgcttatgaatggaaaatgcccaaaaagtcctccttgggcagtatgccgttttcctcagacctcgaggattttgactacagctcttgggatgcaatgtgttatctggatcctagcaaagccgttgaagaggatgactttgtggtgggtttctggaatccatcagaagagaactgtggcgttgacacagggaagcagtccatctcttacgacttgcacacggagcagtgcattgctgacaaaagcatagcggactgtgtggaggccctgctgggctgctatttaaccagctgcggcgagagggccgctcagcttttcctctgctcgctggggctcaaggtgctcccagtcatcaaaaggaccagccgggaaaaggccctgtaccctgctcaggagaacttcagcagccaacaaaagagcctttcagggagctgtgctgccactgcagccagcccacgctcctctgcagggaaagacttggagtatggctgcctgaagatcccaccaaggtgtatgtttgaccatccagatgcagagaaaaccctgaaccacctgatatctgggtttgaaaattttgagaagaaaatcaactacagattcaagaataaggcttaccttctgcaggcttttacacatgcctcctaccactacaacactatcactgattgttaccagcgcttagaattcctgggagatgcgattttggactacctcataaccaagcacctttatgaagacccgcggcagcattccccaggggtcctgactgacctgcgctctgcactggtcaacaacaccatctttgcatcgttggctgtgaagtatgactaccacaagtactttaaagctgtctctcccgagctcttccatgtcattgacgactttgtgcagtttcagcttgagaagaacgagatgcaagggatggactctgagcttaggagatccgaggaggatgaggagaaagaagaggatatcgaagtccccaaggccatgggggacattttcgagtctctcgctggcgccatttatatggacagtgggatgtctctggaggtggtctggcaggtgtactatccaatgatgcggccgctaatagaaaagttttctgccaacgtgccccgttctccagtgagagaattgcttgagatggaaccagaaaccgccaagtttagcccggcggagagaacttacgatgggaaggtcagagtcaccgtggaagtggtgggaaaggggaaatttaaaggtgtcggtcggagctacaggattgcaaagtctgctgcagcaagaagagccctccgcagcctcaaagctaaccarcctcaggttcctaacagctgaaacctccagacgcgcaggcagca

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